Transcription factor PbNAC71 regulates xylem and vessel development to control plant height
© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
Dwarfism is an important agronomic trait in fruit breeding programs. However, the germplasm resources required to generate dwarf pear (Pyrus spp.) varieties are limited. Moreover, the mechanisms underlying dwarfism remain unclear. In this study, 'Yunnan' quince (Cydonia oblonga Mill.) had a dwarfing effect on 'Zaosu' pear. Additionally, the dwarfism-related NAC transcription factor gene PbNAC71 was isolated from pear trees comprising 'Zaosu' (scion) grafted onto 'Yunnan' quince (rootstock). Transgenic Nicotiana benthamiana and pear OHF-333 (Pyrus communis) plants overexpressing PbNAC71 exhibited dwarfism, with a substantially smaller xylem and vessel area relative to the wild-type controls. Yeast one-hybrid, dual-luciferase, chromatin immunoprecipitation-qPCR, and electrophoretic mobility shift assays indicated that PbNAC71 down-regulates PbWalls are thin 1 expression by binding to NAC-binding elements in its promoter. Yeast two-hybrid assays showed that PbNAC71 interacts with the E3 ubiquitin ligase PbRING finger protein 217 (PbRNF217). Furthermore, PbRNF217 promotes the ubiquitin-mediated degradation of PbNAC71 by the 26S proteasome, thereby regulating plant height as well as xylem and vessel development. Our findings reveal a mechanism underlying pear dwarfism and expand our understanding of the molecular basis of dwarfism in woody plants.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Plant physiology - (2024) vom: 10. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cong, Liu [VerfasserIn] |
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Date Revised 10.01.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1093/plphys/kiae011 |
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| PPN (Katalog-ID): |
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| 520 | |a © The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a Dwarfism is an important agronomic trait in fruit breeding programs. However, the germplasm resources required to generate dwarf pear (Pyrus spp.) varieties are limited. Moreover, the mechanisms underlying dwarfism remain unclear. In this study, 'Yunnan' quince (Cydonia oblonga Mill.) had a dwarfing effect on 'Zaosu' pear. Additionally, the dwarfism-related NAC transcription factor gene PbNAC71 was isolated from pear trees comprising 'Zaosu' (scion) grafted onto 'Yunnan' quince (rootstock). Transgenic Nicotiana benthamiana and pear OHF-333 (Pyrus communis) plants overexpressing PbNAC71 exhibited dwarfism, with a substantially smaller xylem and vessel area relative to the wild-type controls. Yeast one-hybrid, dual-luciferase, chromatin immunoprecipitation-qPCR, and electrophoretic mobility shift assays indicated that PbNAC71 down-regulates PbWalls are thin 1 expression by binding to NAC-binding elements in its promoter. Yeast two-hybrid assays showed that PbNAC71 interacts with the E3 ubiquitin ligase PbRING finger protein 217 (PbRNF217). Furthermore, PbRNF217 promotes the ubiquitin-mediated degradation of PbNAC71 by the 26S proteasome, thereby regulating plant height as well as xylem and vessel development. Our findings reveal a mechanism underlying pear dwarfism and expand our understanding of the molecular basis of dwarfism in woody plants | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a dwarf | |
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| 700 | 1 | |a Zhao, Xiao-Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hai-Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Feng-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hong-Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Bai-Quan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhai, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Cheng-Quan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhi-Gang |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Feng-Wang |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Ling-Fei |e verfasserin |4 aut | |
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