Prevalence and molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from dairy cattle with endometritis in Gansu Province, China
© 2024. The Author(s)..
BACKGROUND: The present study aimed to investigate the prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) isolated from dairy cattle with endometritis in China. The prevalence of ESBL-producing E. coli in sample was detected using ChromID ESBL agar, and genotyping of the ESBL producers was performed by PCR and DNA sequencing.
RESULTS: The results revealed that the proportion of positive pathogens tested was 69.76% (180/258) in samples obtained from cows diagnosed with clinical endometritis, with E. coli accounting for 170 out of the 180 positive samples. The infection rate of isolated E. coli was 39.14% (101/258), and co-infections with other pathogens were prevalent. Furthermore, among the 158 E. coli isolates, 50 strains were identified as ESBL producers, with TEM and CTX-M prevalence rates at 78.00% and 32.00%, respectively. Drug sensitivity experiments indicated that 50 isolates of ESBL- producing E. coli were multidrug resistance (MDR), with 48.0% of them exhibiting positive results for both the class 1 integron gene and five gene cassettes associated with resistance to trimethoprim (dfr1 and dfrA17) and aminoglycosides (aadA1, aadA5, and dfrA1), respectively.
CONCLUSION: This investigation demonstrated a substantial prevalence and heightened level of antimicrobial resistance among ESBL-producing E. coli isolates derived from dairy cattle infected with endometritis in China.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
BMC veterinary research - 20(2024), 1 vom: 09. Jan., Seite 19 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Kang [VerfasserIn] |
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| Links: |
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| Themen: |
Beta-Lactamases |
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| Anmerkungen: |
Date Completed 11.01.2024 Date Revised 12.01.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s12917-023-03868-x |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366861263 |
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| 245 | 1 | 0 | |a Prevalence and molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from dairy cattle with endometritis in Gansu Province, China |
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| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024. The Author(s). | ||
| 520 | |a BACKGROUND: The present study aimed to investigate the prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) isolated from dairy cattle with endometritis in China. The prevalence of ESBL-producing E. coli in sample was detected using ChromID ESBL agar, and genotyping of the ESBL producers was performed by PCR and DNA sequencing | ||
| 520 | |a RESULTS: The results revealed that the proportion of positive pathogens tested was 69.76% (180/258) in samples obtained from cows diagnosed with clinical endometritis, with E. coli accounting for 170 out of the 180 positive samples. The infection rate of isolated E. coli was 39.14% (101/258), and co-infections with other pathogens were prevalent. Furthermore, among the 158 E. coli isolates, 50 strains were identified as ESBL producers, with TEM and CTX-M prevalence rates at 78.00% and 32.00%, respectively. Drug sensitivity experiments indicated that 50 isolates of ESBL- producing E. coli were multidrug resistance (MDR), with 48.0% of them exhibiting positive results for both the class 1 integron gene and five gene cassettes associated with resistance to trimethoprim (dfr1 and dfrA17) and aminoglycosides (aadA1, aadA5, and dfrA1), respectively | ||
| 520 | |a CONCLUSION: This investigation demonstrated a substantial prevalence and heightened level of antimicrobial resistance among ESBL-producing E. coli isolates derived from dairy cattle infected with endometritis in China | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Dairy cattle | |
| 650 | 4 | |a Endometritis | |
| 650 | 4 | |a Escherichia coli | |
| 650 | 4 | |a Extended-spectrum β-lactamase | |
| 650 | 4 | |a Molecular characterization | |
| 650 | 4 | |a Prevalence | |
| 650 | 7 | |a beta-Lactamases |2 NLM | |
| 650 | 7 | |a EC 3.5.2.6 |2 NLM | |
| 700 | 1 | |a Feng, Haipeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jingyan |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Zhiting |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Zunxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Guibo |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xuezhi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jianxi |e verfasserin |4 aut | |
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