Identification of a spike-specific CD8+ T cell epitope following vaccination against the Middle East respiratory syndrome coronavirus in humans
© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America..
Licensed vaccines against the Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging pathogen of concern, are lacking. The Modified Vaccinia virus Ankara vector-based vaccine MVA-MERS-S, expressing the MERS-CoV-spike glycoprotein (MERS-S), is one of three candidate vaccines in clinical development and elicits robust humoral and cellular immunity. Here, we identified for the first time a MERS-S-specific CD8+ T-cell epitope in an HLA-A*03:01/HLA-B*35:01-positive vaccinee using a screening assay, intracellular cytokine staining, and in silico epitope prediction. As evidence from MERS-CoV infection suggests a protective role of long-lasting CD8+ T-cell responses, the identification of epitopes will facilitate longitudinal analyses of vaccine-induced T-cell immunity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
The Journal of infectious diseases - (2024) vom: 09. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Harrer, Caroline E [VerfasserIn] |
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| Links: |
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| Themen: |
CD8+ T cells |
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| Anmerkungen: |
Date Revised 09.01.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1093/infdis/jiad612 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366858076 |
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| 245 | 1 | 0 | |a Identification of a spike-specific CD8+ T cell epitope following vaccination against the Middle East respiratory syndrome coronavirus in humans |
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| 520 | |a © The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. | ||
| 520 | |a Licensed vaccines against the Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging pathogen of concern, are lacking. The Modified Vaccinia virus Ankara vector-based vaccine MVA-MERS-S, expressing the MERS-CoV-spike glycoprotein (MERS-S), is one of three candidate vaccines in clinical development and elicits robust humoral and cellular immunity. Here, we identified for the first time a MERS-S-specific CD8+ T-cell epitope in an HLA-A*03:01/HLA-B*35:01-positive vaccinee using a screening assay, intracellular cytokine staining, and in silico epitope prediction. As evidence from MERS-CoV infection suggests a protective role of long-lasting CD8+ T-cell responses, the identification of epitopes will facilitate longitudinal analyses of vaccine-induced T-cell immunity | ||
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| 700 | 1 | |a Mayer, Leonie |e verfasserin |4 aut | |
| 700 | 1 | |a Fathi, Anahita |e verfasserin |4 aut | |
| 700 | 1 | |a Lassen, Susan |e verfasserin |4 aut | |
| 700 | 1 | |a Ly, My L |e verfasserin |4 aut | |
| 700 | 1 | |a Zinser, Madeleine E |e verfasserin |4 aut | |
| 700 | 1 | |a Wolf, Timo |e verfasserin |4 aut | |
| 700 | 1 | |a Becker, Stephan |e verfasserin |4 aut | |
| 700 | 1 | |a Sutter, Gerd |e verfasserin |4 aut | |
| 700 | 1 | |a Dahlke, Christine |e verfasserin |4 aut | |
| 700 | 1 | |a Addo, Marylyn M |e verfasserin |4 aut | |
| 700 | 0 | |a MVA-MERS-S Study Group |e verfasserin |4 aut | |
| 700 | 1 | |a Bartels, Etienne |e investigator |4 oth | |
| 700 | 1 | |a Gundlach, Swantje |e investigator |4 oth | |
| 700 | 1 | |a Haagmanns, Bart |e investigator |4 oth | |
| 700 | 1 | |a Hesterkamp, Thomas |e investigator |4 oth | |
| 700 | 1 | |a Koch, Till |e investigator |4 oth | |
| 700 | 1 | |a Krähling, Verena |e investigator |4 oth | |
| 700 | 1 | |a Poetsch, Joseph |e investigator |4 oth | |
| 700 | 1 | |a Schmiedel, Stefan |e investigator |4 oth | |
| 700 | 1 | |a Volz, Asisa |e investigator |4 oth | |
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