Ionic Liquid-Mediated Dynamic Polymerization for Facile Aqueous-Phase Synthesis of Enzyme-Covalent Organic Framework Biocatalysts
© 2024 Wiley-VCH GmbH..
Utilizing covalent organic framework (COF) as a hypotoxic and porous scaffold to encapsulate enzyme (enzymeCOF) has inspired numerous interests at the intersection of chemistry, materials, and biological science. In this study, we report a convenient scheme for one-step, aqueous-phase synthesis of highly crystalline enzyme@COF biocatalysts. This facile approach relies on an ionic liquid (2 μL of imidazolium ionic liquid)-mediated dynamic polymerization mechanism, which can facilitate the in situ assembly of enzyme@COF under mild conditions. This green strategy is adaptive to synthesize different biocatalysts with highly crystalline COF "exoskeleton", as well evidenced by the low-dose cryo-EM and other characterizations. Attributing to the rigorous sieving effect of crystalline COF pore, the hosted lipase shows non-native selectivity for aliphatic acid hydrolysis. In addition, the highly crystalline linkage affords COF "exoskeleton" with higher photocatalytic activity for in situ production of H2 O2 , enabling us to construct a self-cascading photo-enzyme coupled reactor for pollutants degradation, with a 2.63-fold degradation rate as the poorly crystalline photo-enzyme reactor. This work showcases the great potentials of employing green and trace amounts of ionic liquid for one-step synthesis of crystalline enzyme@COF biocatalysts, and emphasizes the feasibility of diversifying enzyme functions by integrating the reticular chemistry of a COF.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
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| Enthalten in: |
Angewandte Chemie (International ed. in English) - 63(2024), 8 vom: 19. Feb., Seite e202319876 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gao, Rui [VerfasserIn] |
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| Links: |
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| Themen: |
Covalent Organic Frameworks |
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| Anmerkungen: |
Date Completed 14.02.2024 Date Revised 14.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/anie.202319876 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM366739832 |
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| 520 | |a Utilizing covalent organic framework (COF) as a hypotoxic and porous scaffold to encapsulate enzyme (enzymeCOF) has inspired numerous interests at the intersection of chemistry, materials, and biological science. In this study, we report a convenient scheme for one-step, aqueous-phase synthesis of highly crystalline enzyme@COF biocatalysts. This facile approach relies on an ionic liquid (2 μL of imidazolium ionic liquid)-mediated dynamic polymerization mechanism, which can facilitate the in situ assembly of enzyme@COF under mild conditions. This green strategy is adaptive to synthesize different biocatalysts with highly crystalline COF "exoskeleton", as well evidenced by the low-dose cryo-EM and other characterizations. Attributing to the rigorous sieving effect of crystalline COF pore, the hosted lipase shows non-native selectivity for aliphatic acid hydrolysis. In addition, the highly crystalline linkage affords COF "exoskeleton" with higher photocatalytic activity for in situ production of H2 O2 , enabling us to construct a self-cascading photo-enzyme coupled reactor for pollutants degradation, with a 2.63-fold degradation rate as the poorly crystalline photo-enzyme reactor. This work showcases the great potentials of employing green and trace amounts of ionic liquid for one-step synthesis of crystalline enzyme@COF biocatalysts, and emphasizes the feasibility of diversifying enzyme functions by integrating the reticular chemistry of a COF | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Covalent Organic Frameworks | |
| 650 | 4 | |a Enzyme Immobilization | |
| 650 | 4 | |a Ionic Liquid | |
| 650 | 4 | |a Pollutants Degradation | |
| 650 | 7 | |a Ionic Liquids |2 NLM | |
| 650 | 7 | |a Metal-Organic Frameworks |2 NLM | |
| 650 | 7 | |a Lipase |2 NLM | |
| 650 | 7 | |a EC 3.1.1.3 |2 NLM | |
| 700 | 1 | |a Kou, Xiaoxue |e verfasserin |4 aut | |
| 700 | 1 | |a Tong, Linjing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zhi-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Yujian |e verfasserin |4 aut | |
| 700 | 1 | |a He, Rongwei |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Lihong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Xiaomin |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Siming |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Guosheng |e verfasserin |4 aut | |
| 700 | 1 | |a Ouyang, Gangfeng |e verfasserin |4 aut | |
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