The Actin Cytoskeleton as a Regulator of Proteoglycan 4
OBJECTIVE: The superficial zone (SZ) of articular cartilage is responsible for distributing shear forces for optimal cartilage loading and contributes to joint lubrication through the production of proteoglycan 4 (PRG4). PRG4 plays a critical role in joint homeostasis and is chondroprotective. Normal PRG4 production is affected by inflammation and irregular mechanical loading in post-traumatic osteoarthritis (PTOA). THe SZ chondrocyte (SZC) phenotype, including PRG4 expression, is regulated by the actin cytoskeleton in vitro. There remains a limited understanding of the regulation of PRG4 by the actin cytoskeleton in native articular chondrocytes. The filamentous (F)-actin cytoskeleton is a potential node in crosstalk between mechanical stimulation and cytokine activation and the regulation of PRG4 in SZCs, therefore developing insights in the regulation of PRG4 by actin may identify molecular targets for novel PTOA therapies.
MATERIALS AND METHODS: A comprehensive literature search on PRG4 and the regulation of the SZC phenotype by actin organization was performed.
RESULTS: PRG4 is strongly regulated by the actin cytoskeleton in isolated SZCs in vitro. Biochemical and mechanical stimuli have been characterized to regulate PRG4 and may converge upon actin cytoskeleton signaling.
CONCLUSION: Actin-based regulation of PRG4 in native SZCs is not fully understood and requires further elucidation. Understanding the regulation of PRG4 by actin in SZCs requires an in vivo context to further potential of leveraging actin arrangement to arthritic therapeutics.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
|---|---|
| Enthalten in: |
Cartilage - (2024) vom: 06. Jan., Seite 19476035231223455 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Gonzalez-Nolde, Sofia [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Actin cytoskeleton |
|---|
| Anmerkungen: |
Date Revised 06.01.2024 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1177/19476035231223455 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366738461 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366738461 | ||
| 003 | DE-627 | ||
| 005 | 20240108142743.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240108s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1177/19476035231223455 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1250.xml |
| 035 | |a (DE-627)NLM366738461 | ||
| 035 | |a (NLM)38183234 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Gonzalez-Nolde, Sofia |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Actin Cytoskeleton as a Regulator of Proteoglycan 4 |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 06.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a OBJECTIVE: The superficial zone (SZ) of articular cartilage is responsible for distributing shear forces for optimal cartilage loading and contributes to joint lubrication through the production of proteoglycan 4 (PRG4). PRG4 plays a critical role in joint homeostasis and is chondroprotective. Normal PRG4 production is affected by inflammation and irregular mechanical loading in post-traumatic osteoarthritis (PTOA). THe SZ chondrocyte (SZC) phenotype, including PRG4 expression, is regulated by the actin cytoskeleton in vitro. There remains a limited understanding of the regulation of PRG4 by the actin cytoskeleton in native articular chondrocytes. The filamentous (F)-actin cytoskeleton is a potential node in crosstalk between mechanical stimulation and cytokine activation and the regulation of PRG4 in SZCs, therefore developing insights in the regulation of PRG4 by actin may identify molecular targets for novel PTOA therapies | ||
| 520 | |a MATERIALS AND METHODS: A comprehensive literature search on PRG4 and the regulation of the SZC phenotype by actin organization was performed | ||
| 520 | |a RESULTS: PRG4 is strongly regulated by the actin cytoskeleton in isolated SZCs in vitro. Biochemical and mechanical stimuli have been characterized to regulate PRG4 and may converge upon actin cytoskeleton signaling | ||
| 520 | |a CONCLUSION: Actin-based regulation of PRG4 in native SZCs is not fully understood and requires further elucidation. Understanding the regulation of PRG4 by actin in SZCs requires an in vivo context to further potential of leveraging actin arrangement to arthritic therapeutics | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a actin cytoskeleton | |
| 650 | 4 | |a lubricin | |
| 650 | 4 | |a post-traumatic osteoarthritis | |
| 650 | 4 | |a proteoglycan 4 | |
| 650 | 4 | |a superficial zone chondrocytes | |
| 700 | 1 | |a Schweiger, Cameron J |e verfasserin |4 aut | |
| 700 | 1 | |a Davis, Elizabeth E R |e verfasserin |4 aut | |
| 700 | 1 | |a Manzoni, Thomas J |e verfasserin |4 aut | |
| 700 | 1 | |a Hussein, Samer M I |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidt, Tannin A |e verfasserin |4 aut | |
| 700 | 1 | |a Cone, Stephanie G |e verfasserin |4 aut | |
| 700 | 1 | |a Jay, Gregory D |e verfasserin |4 aut | |
| 700 | 1 | |a Parreno, Justin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cartilage |d 2010 |g (2024) vom: 06. Jan., Seite 19476035231223455 |w (DE-627)NLM199891206 |x 1947-6043 |7 nnns |
| 773 | 1 | 8 | |g year:2024 |g day:06 |g month:01 |g pages:19476035231223455 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1177/19476035231223455 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 06 |c 01 |h 19476035231223455 | ||