The male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved..
Dynamic chromosome remodeling and nuclear compartmentalization take place during mammalian meiotic prophase I. We report here that the crucial roles of male pachynema-specific protein (MAPS) in pachynema progression might be mediated by its liquid-liquid phase separation in vitro and in cellulo. MAPS forms distinguishable liquid phases, and deletion or mutations of its N-terminal amino acids (aa) 2-9 disrupt its secondary structure and charge properties, impeding phase separation. Maps-/- pachytene spermatocytes exhibit defects in nucleus compartmentalization, including defects in forming sex bodies, altered nucleosome composition, and disordered chromatin accessibility. MapsΔ2-9/Δ2-9 male mice expressing MAPS protein lacking aa 2-9 phenocopy Maps-/- mice. Moreover, a frameshift mutation in C3orf62, the human counterpart of Maps, is correlated with nonobstructive azoospermia in a patient exhibiting pachynema arrest in spermatocyte development. Hence, the phase separation property of MAPS seems essential for pachynema progression in mouse and human spermatocytes.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
|---|---|
| Enthalten in: |
Cell reports - 43(2024), 1 vom: 23. Jan., Seite 113651 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lin, Zexiong [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
CP: Cell biology |
|---|
| Anmerkungen: |
Date Completed 26.01.2024 Date Revised 12.02.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.celrep.2023.113651 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366663763 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366663763 | ||
| 003 | DE-627 | ||
| 005 | 20240213232729.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240108s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.celrep.2023.113651 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1290.xml |
| 035 | |a (DE-627)NLM366663763 | ||
| 035 | |a (NLM)38175751 | ||
| 035 | |a (PII)S2211-1247(23)01662-5 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lin, Zexiong |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.01.2024 | ||
| 500 | |a Date Revised 12.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Dynamic chromosome remodeling and nuclear compartmentalization take place during mammalian meiotic prophase I. We report here that the crucial roles of male pachynema-specific protein (MAPS) in pachynema progression might be mediated by its liquid-liquid phase separation in vitro and in cellulo. MAPS forms distinguishable liquid phases, and deletion or mutations of its N-terminal amino acids (aa) 2-9 disrupt its secondary structure and charge properties, impeding phase separation. Maps-/- pachytene spermatocytes exhibit defects in nucleus compartmentalization, including defects in forming sex bodies, altered nucleosome composition, and disordered chromatin accessibility. MapsΔ2-9/Δ2-9 male mice expressing MAPS protein lacking aa 2-9 phenocopy Maps-/- mice. Moreover, a frameshift mutation in C3orf62, the human counterpart of Maps, is correlated with nonobstructive azoospermia in a patient exhibiting pachynema arrest in spermatocyte development. Hence, the phase separation property of MAPS seems essential for pachynema progression in mouse and human spermatocytes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CP: Cell biology | |
| 650 | 4 | |a CP: Molecular biology | |
| 650 | 4 | |a MAPS | |
| 650 | 4 | |a male infertility | |
| 650 | 4 | |a meiotic prophase I | |
| 650 | 4 | |a phase separation | |
| 650 | 4 | |a sex body formation | |
| 650 | 7 | |a Chromatin |2 NLM | |
| 700 | 1 | |a Li, Dongliang |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Jiahuan |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Chencheng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Dongteng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Haiwei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Chunxu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yuxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Binjie |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Fu |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Dandan |e verfasserin |4 aut | |
| 700 | 1 | |a Rodriguez-Wallberg, Kenny A |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Yeung, William S B |e verfasserin |4 aut | |
| 700 | 1 | |a Chow, Louise T |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hengbin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Kui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell reports |d 2012 |g 43(2024), 1 vom: 23. Jan., Seite 113651 |w (DE-627)NLM217067492 |x 2211-1247 |7 nnns |
| 773 | 1 | 8 | |g volume:43 |g year:2024 |g number:1 |g day:23 |g month:01 |g pages:113651 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.celrep.2023.113651 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 43 |j 2024 |e 1 |b 23 |c 01 |h 113651 | ||