The importance of biofilm contamination control for dental unit waterlines : a multicenter assessment of the microbiota diversity of biofilm in dental unit waterlines
© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group..
Background: The biofilm formation in Dental Unit Waterlines (DUWLs) could become an important cause of infection during dental care, which could put immunocompromised individuals at risk of cross-infection. The aim of this study was to characterize the microbial communities of biofilms among DUWLs using high-throughput sequencing technology.
Methods: Twenty-nine biofilm samples were obtained from 24 dental chair units at 5 hospitals and 2 dental clinics. The genomic DNA of the samples was extracted, then 16S rDNA and ITS2 gene were amplified and sequenced. Alpha-diversity and Beta-diversity were calculated with QIIME2 and the Kruskal - Wallis H-test was adopted for statistical analysis.
Results: Microbial communities with a high diversity of bacteria (377 genera) and fungi (83 genera) were detected in the biofilm samples. The dominant phylum of bacteria was Proteobacteria (93.27%) and that of fungi was Basidiomycota (68.15%). Potential human pathogens were detected including 7 genera of bacteria (Pseudomonas, Stenotrophomonas, Hafnia-Obesumbacterium, Burkholderia-Caballeronia-Paraburkholderia, Ralstonia, Enterobacter, Klebsiella) and 6 genera of fungi (Malassezia, Candida, Alternaria, Cryptococcus, Rhodotorula, Rhinocladiella).
Conclusions: This multicenter assessment revealed the infectious risk during dental care. It emphasized the importance of biofilm control due to biofilm accumulation and multiple kinds of opportunistic pathogens in DUWLs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Journal of oral microbiology - 16(2024), 1 vom: 22., Seite 2299496 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Piaopiao [VerfasserIn] |
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| Links: |
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| Themen: |
Biofilm |
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| Anmerkungen: |
Date Revised 05.01.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1080/20002297.2023.2299496 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366647385 |
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| 520 | |a © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. | ||
| 520 | |a Background: The biofilm formation in Dental Unit Waterlines (DUWLs) could become an important cause of infection during dental care, which could put immunocompromised individuals at risk of cross-infection. The aim of this study was to characterize the microbial communities of biofilms among DUWLs using high-throughput sequencing technology | ||
| 520 | |a Methods: Twenty-nine biofilm samples were obtained from 24 dental chair units at 5 hospitals and 2 dental clinics. The genomic DNA of the samples was extracted, then 16S rDNA and ITS2 gene were amplified and sequenced. Alpha-diversity and Beta-diversity were calculated with QIIME2 and the Kruskal - Wallis H-test was adopted for statistical analysis | ||
| 520 | |a Results: Microbial communities with a high diversity of bacteria (377 genera) and fungi (83 genera) were detected in the biofilm samples. The dominant phylum of bacteria was Proteobacteria (93.27%) and that of fungi was Basidiomycota (68.15%). Potential human pathogens were detected including 7 genera of bacteria (Pseudomonas, Stenotrophomonas, Hafnia-Obesumbacterium, Burkholderia-Caballeronia-Paraburkholderia, Ralstonia, Enterobacter, Klebsiella) and 6 genera of fungi (Malassezia, Candida, Alternaria, Cryptococcus, Rhodotorula, Rhinocladiella) | ||
| 520 | |a Conclusions: This multicenter assessment revealed the infectious risk during dental care. It emphasized the importance of biofilm control due to biofilm accumulation and multiple kinds of opportunistic pathogens in DUWLs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Dental unit waterlines | |
| 650 | 4 | |a biofilm | |
| 650 | 4 | |a high-throughput sequencing | |
| 650 | 4 | |a infection control | |
| 650 | 4 | |a microbial contamination | |
| 650 | 4 | |a pathogens | |
| 700 | 1 | |a Zeng, Jiang |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Feiruo |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Cong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huimin |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xuefen |e verfasserin |4 aut | |
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