Evaluation of Bis(2-ethylhexyl) phthalate toxicity on the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9
Copyright © 2024 Elsevier Ltd. All rights reserved..
Bis(2-ethylhexyl) phthalate, generally known as DEHP is a synthetic compound mainly used as a plasticizer to make polyvinyl chloride products flexible and soft. The present work aimed to study the toxicity of Bis(2-ethylhexyl) phthalate on the third instar larvae of transgenic Drosophila melanogaster(hsp70-lacZ) Bg9. The hsp70 gene is associated with the β-galactosidase in our present transgenic strain therefore, the more activity of β-galactosidase will indirectly correspond to hsp70 expression. The third instar larvae were allowed to feed on the diet for 24 h having 0.001, 0.005, 0.01, and 0.02 M of Bis(2-ethylhexyl) phthalate at the final concentration. After the exposure of 24hrs, the larvae were subjected to ONPG assay, X-gal staining, trypan blue exclusion test, oxidative stress markers assays, and comet assay. A dose-dependent increase in hsp70 expression, tissue damage, Glutathione-S-transferase (GST) activity, lipid peroxidation, monoamine oxidase, caspase-9 & 3, protein carbonyl content (PCC), DNA damage and decrease in the glutathione (GSH) content, delta-aminolevulinic acid dehydrogenase (ẟ-ALD-D) and acetylcholinesterase activity were observed in the larvae exposed to 0.005, 0.01, 0.02 M of Bis-(2-ethylhexyl) phthalate. The dose of 0.001 M of Bis(2-ethylhexyl) phthalate did not showed any toxic effects and hence can be considered as No Observed Adverse Effect Level (NOAEL) for Bis(2-ethylhexyl) phthalate. The study supports the use of Drosophila for the evaluation of possible toxic effects associated with synthetic compounds.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:184 |
---|---|
Enthalten in: |
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association - 184(2024) vom: 30. Jan., Seite 114425 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Gaur, Kajal [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 29.01.2024 Date Revised 29.01.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.fct.2023.114425 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM366514148 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM366514148 | ||
003 | DE-627 | ||
005 | 20240129232044.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240108s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.fct.2023.114425 |2 doi | |
028 | 5 | 2 | |a pubmed24n1274.xml |
035 | |a (DE-627)NLM366514148 | ||
035 | |a (NLM)38160779 | ||
035 | |a (PII)S0278-6915(23)00828-1 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Gaur, Kajal |e verfasserin |4 aut | |
245 | 1 | 0 | |a Evaluation of Bis(2-ethylhexyl) phthalate toxicity on the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9 |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 29.01.2024 | ||
500 | |a Date Revised 29.01.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2024 Elsevier Ltd. All rights reserved. | ||
520 | |a Bis(2-ethylhexyl) phthalate, generally known as DEHP is a synthetic compound mainly used as a plasticizer to make polyvinyl chloride products flexible and soft. The present work aimed to study the toxicity of Bis(2-ethylhexyl) phthalate on the third instar larvae of transgenic Drosophila melanogaster(hsp70-lacZ) Bg9. The hsp70 gene is associated with the β-galactosidase in our present transgenic strain therefore, the more activity of β-galactosidase will indirectly correspond to hsp70 expression. The third instar larvae were allowed to feed on the diet for 24 h having 0.001, 0.005, 0.01, and 0.02 M of Bis(2-ethylhexyl) phthalate at the final concentration. After the exposure of 24hrs, the larvae were subjected to ONPG assay, X-gal staining, trypan blue exclusion test, oxidative stress markers assays, and comet assay. A dose-dependent increase in hsp70 expression, tissue damage, Glutathione-S-transferase (GST) activity, lipid peroxidation, monoamine oxidase, caspase-9 & 3, protein carbonyl content (PCC), DNA damage and decrease in the glutathione (GSH) content, delta-aminolevulinic acid dehydrogenase (ẟ-ALD-D) and acetylcholinesterase activity were observed in the larvae exposed to 0.005, 0.01, 0.02 M of Bis-(2-ethylhexyl) phthalate. The dose of 0.001 M of Bis(2-ethylhexyl) phthalate did not showed any toxic effects and hence can be considered as No Observed Adverse Effect Level (NOAEL) for Bis(2-ethylhexyl) phthalate. The study supports the use of Drosophila for the evaluation of possible toxic effects associated with synthetic compounds | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a DNA damage | |
650 | 4 | |a HSP 70 | |
650 | 4 | |a Oxidative stress markers | |
650 | 4 | |a Phthalates | |
650 | 4 | |a Toxicity | |
650 | 7 | |a phthalic acid |2 NLM | |
650 | 7 | |a 6O7F7IX66E |2 NLM | |
650 | 7 | |a Acetylcholinesterase |2 NLM | |
650 | 7 | |a EC 3.1.1.7 |2 NLM | |
650 | 7 | |a Glutathione |2 NLM | |
650 | 7 | |a GAN16C9B8O |2 NLM | |
650 | 7 | |a beta-Galactosidase |2 NLM | |
650 | 7 | |a EC 3.2.1.23 |2 NLM | |
650 | 7 | |a Diethylhexyl Phthalate |2 NLM | |
650 | 7 | |a C42K0PH13C |2 NLM | |
650 | 7 | |a Phthalic Acids |2 NLM | |
700 | 1 | |a Varshney, Himanshi |e verfasserin |4 aut | |
700 | 1 | |a Subhan, Iqra |e verfasserin |4 aut | |
700 | 1 | |a Fatima, Javeria |e verfasserin |4 aut | |
700 | 1 | |a Jyoti, Smita |e verfasserin |4 aut | |
700 | 1 | |a Siddique, Yasir Hasan |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association |d 1982 |g 184(2024) vom: 30. Jan., Seite 114425 |w (DE-627)NLM012624691 |x 1873-6351 |7 nnns |
773 | 1 | 8 | |g volume:184 |g year:2024 |g day:30 |g month:01 |g pages:114425 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.fct.2023.114425 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 184 |j 2024 |b 30 |c 01 |h 114425 |