Amphotericin B loaded nanoemulsion : Optimization, characterization and in-vitro activity against L. donovani promastigotes
Copyright © 2023. Published by Elsevier B.V..
The present work aimed to develop and evaluate AmB-loaded nano-emulsion (AmB-NE) which will augment the solubility of AmB and lead to enhanced anti-leishmanial activity. The composition of AmB-NE was optimized by systematic screening followed by DoE-extreme vertices mixture design. The optimized NE revealed mean droplet size and PDI of 44.19 ± 5.5 nm, 0.265 ± 0.0723, respectively. The NE could efficiently encapsulate AmB with drug content and efficiency 83.509 ± 0.369% and 81.659 ± 0.013%, respectively. The presence of cholesterol and stearyl amine retarded the release (P < 0.0001) of AmB significantly compared to AmB suspension. The AmB-NE and pure AmB suspension demonstrated the IC50 of 0.06309 μg/mL and 0.3309 μg/mL against L.donovani promastigotes after 48 h incubation. The formulation was robust at all exaggerated stability conditions such as freeze-thaw and centrifugation. These findings indicate that AmB-NE is an attractive approach to treat visceral leishmaniasis with improved activity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:100 |
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| Enthalten in: |
Parasitology international - 100(2024) vom: 01. März, Seite 102848 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Prajapat, Vikram Mohanlal [VerfasserIn] |
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| Links: |
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| Themen: |
1,8-cineol oil |
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| Anmerkungen: |
Date Completed 18.03.2024 Date Revised 18.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.parint.2023.102848 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023. Published by Elsevier B.V. | ||
| 520 | |a The present work aimed to develop and evaluate AmB-loaded nano-emulsion (AmB-NE) which will augment the solubility of AmB and lead to enhanced anti-leishmanial activity. The composition of AmB-NE was optimized by systematic screening followed by DoE-extreme vertices mixture design. The optimized NE revealed mean droplet size and PDI of 44.19 ± 5.5 nm, 0.265 ± 0.0723, respectively. The NE could efficiently encapsulate AmB with drug content and efficiency 83.509 ± 0.369% and 81.659 ± 0.013%, respectively. The presence of cholesterol and stearyl amine retarded the release (P < 0.0001) of AmB significantly compared to AmB suspension. The AmB-NE and pure AmB suspension demonstrated the IC50 of 0.06309 μg/mL and 0.3309 μg/mL against L.donovani promastigotes after 48 h incubation. The formulation was robust at all exaggerated stability conditions such as freeze-thaw and centrifugation. These findings indicate that AmB-NE is an attractive approach to treat visceral leishmaniasis with improved activity | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Amphotericin B | |
| 650 | 4 | |a Antifungal activity | |
| 650 | 4 | |a Antileishmanial activity | |
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| 700 | 1 | |a Sriram, Anitha |e verfasserin |4 aut | |
| 700 | 1 | |a Mahajan, Srushti |e verfasserin |4 aut | |
| 700 | 1 | |a Maji, Indrani |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Ujala |e verfasserin |4 aut | |
| 700 | 1 | |a Kumari, Diksha |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Kuljit |e verfasserin |4 aut | |
| 700 | 1 | |a Kalia, Nitin Pal |e verfasserin |4 aut | |
| 700 | 1 | |a Dua, Kamal |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Sachin Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Pankaj Kumar |e verfasserin |4 aut | |
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