Filamentous-Actin-Mimicking Nanoplatform for Enhanced Cytosolic Protein Delivery
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH..
Despite the potential of protein therapeutics, the cytosolic delivery of proteins with high efficiency and bioactivity remains a significant challenge owing to exocytosis and lysosomal degradation after endocytosis. Therefore, it is important to develop a safe and efficient strategy to bypass endocytosis. Inspired by the extraordinary capability of filamentous-actin (F-actin) to promote cell membrane fusion, a cyanine dye assembly-containing nanoplatform mimicking the structure of natural F-actin is developed. The nanoplatform exhibits fast membrane fusion to cell membrane mimics and thus enters live cells through membrane fusion and bypasses endocytosis. Moreover, it is found to efficiently deliver protein cargos into live cells and quickly release them into the cytosol, leading to high protein cargo transfection efficiency and bioactivity. The nanoplatform also results in the superior inhibition of tumor cells when loaded with anti-tumor proteins. These results demonstrate that this fusogenic nanoplatform can be valuable for cytosolic protein delivery and tumor treatment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Advanced science (Weinheim, Baden-Wurttemberg, Germany) - 11(2024), 10 vom: 28. März, Seite e2305600 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xia, Yuqiong [VerfasserIn] |
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| Links: |
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| Themen: |
Actins |
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| Anmerkungen: |
Date Completed 14.03.2024 Date Revised 15.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/advs.202305600 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM366436147 |
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| 520 | |a Despite the potential of protein therapeutics, the cytosolic delivery of proteins with high efficiency and bioactivity remains a significant challenge owing to exocytosis and lysosomal degradation after endocytosis. Therefore, it is important to develop a safe and efficient strategy to bypass endocytosis. Inspired by the extraordinary capability of filamentous-actin (F-actin) to promote cell membrane fusion, a cyanine dye assembly-containing nanoplatform mimicking the structure of natural F-actin is developed. The nanoplatform exhibits fast membrane fusion to cell membrane mimics and thus enters live cells through membrane fusion and bypasses endocytosis. Moreover, it is found to efficiently deliver protein cargos into live cells and quickly release them into the cytosol, leading to high protein cargo transfection efficiency and bioactivity. The nanoplatform also results in the superior inhibition of tumor cells when loaded with anti-tumor proteins. These results demonstrate that this fusogenic nanoplatform can be valuable for cytosolic protein delivery and tumor treatment | ||
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| 700 | 1 | |a Liu, Chang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Xuejuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Jianxia |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zhaoxu |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Minchao |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Cheng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xianghan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhongliang |e verfasserin |4 aut | |
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