Rutin attenuates inflammation by downregulating AGE-RAGE signaling pathway in psoriasis : Network pharmacology analysis and experimental evidence
Copyright © 2023 Elsevier B.V. All rights reserved..
BACKGROUND: Jueyin granules (JYG) is effective against psoriasis, but its utility components are not clear. Rutin is the main monomer of JYG, its therapeutic effect and mechanism on psoriasis need to be further clarified.
PURPOSE: To explore the potential mechanisms of rutin on psoriasis through network pharmacology and experiments.
METHODS: In vitro, cell viability was determined using the CCK8 assay, and inflammatory factors were identified using RT-qPCR. The hub genes and kernel pathways of action were identified by modular pharmacology analysis. In vivo, a BALB/c mice model of psoriasis was induced by Imiquimod (IMQ). The therapeutic effect and action pathway were detected through Western Blotting, RT-qPCR, histopathologic and immunohistochemical analysis.
RESULTS: Rutin inhibited cell proliferation and expression of TNF-α and IL-6 in HaCaT cells. The hub genes include APP, INS, and TNF, while the kernel pathways contain the AGE-RAGE signaling pathway. In IMQ-induced psoriasis-like mice, rutin ameliorated skin lesions and inhibited cell proliferation. Rutin could attenuate inflammation by downregulating the AGE-RAGE signaling pathway.
CONCLUSION: This study suggests that rutin can reduce IMQ-induced psoriasis like skin inflammation in mice, and regulation of AGE-RAGE signaling pathway may be one of its potential anti-inflammatory mechanisms. Rutin has a promising therapeutic use for the treatment of psoriasis.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:125 |
|---|---|
| Enthalten in: |
International immunopharmacology - 125(2023), Pt A vom: 27. Dez., Seite 111033 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Mingxia [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
5G06TVY3R7 |
|---|
| Anmerkungen: |
Date Completed 28.12.2023 Date Revised 28.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.intimp.2023.111033 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366402234 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366402234 | ||
| 003 | DE-627 | ||
| 005 | 20231229124301.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231229s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.intimp.2023.111033 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1241.xml |
| 035 | |a (DE-627)NLM366402234 | ||
| 035 | |a (NLM)38149569 | ||
| 035 | |a (PII)S1567-5769(23)01358-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Mingxia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Rutin attenuates inflammation by downregulating AGE-RAGE signaling pathway in psoriasis |b Network pharmacology analysis and experimental evidence |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 28.12.2023 | ||
| 500 | |a Date Revised 28.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: Jueyin granules (JYG) is effective against psoriasis, but its utility components are not clear. Rutin is the main monomer of JYG, its therapeutic effect and mechanism on psoriasis need to be further clarified | ||
| 520 | |a PURPOSE: To explore the potential mechanisms of rutin on psoriasis through network pharmacology and experiments | ||
| 520 | |a METHODS: In vitro, cell viability was determined using the CCK8 assay, and inflammatory factors were identified using RT-qPCR. The hub genes and kernel pathways of action were identified by modular pharmacology analysis. In vivo, a BALB/c mice model of psoriasis was induced by Imiquimod (IMQ). The therapeutic effect and action pathway were detected through Western Blotting, RT-qPCR, histopathologic and immunohistochemical analysis | ||
| 520 | |a RESULTS: Rutin inhibited cell proliferation and expression of TNF-α and IL-6 in HaCaT cells. The hub genes include APP, INS, and TNF, while the kernel pathways contain the AGE-RAGE signaling pathway. In IMQ-induced psoriasis-like mice, rutin ameliorated skin lesions and inhibited cell proliferation. Rutin could attenuate inflammation by downregulating the AGE-RAGE signaling pathway | ||
| 520 | |a CONCLUSION: This study suggests that rutin can reduce IMQ-induced psoriasis like skin inflammation in mice, and regulation of AGE-RAGE signaling pathway may be one of its potential anti-inflammatory mechanisms. Rutin has a promising therapeutic use for the treatment of psoriasis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AGE-RAGE signaling pathway | |
| 650 | 4 | |a Network pharmacology | |
| 650 | 4 | |a Psoriasis | |
| 650 | 4 | |a Rutin | |
| 650 | 7 | |a Rutin |2 NLM | |
| 650 | 7 | |a 5G06TVY3R7 |2 NLM | |
| 650 | 7 | |a Imiquimod |2 NLM | |
| 650 | 7 | |a P1QW714R7M |2 NLM | |
| 700 | 1 | |a Ma, Xiaoxuan |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Chunjie |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Fei, Xiaoya |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Kuai, Le |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ruiping |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jiankun |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International immunopharmacology |d 2001 |g 125(2023), Pt A vom: 27. Dez., Seite 111033 |w (DE-627)NLM112639542 |x 1878-1705 |7 nnns |
| 773 | 1 | 8 | |g volume:125 |g year:2023 |g number:Pt A |g day:27 |g month:12 |g pages:111033 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.intimp.2023.111033 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 125 |j 2023 |e Pt A |b 27 |c 12 |h 111033 | ||