Decreased SMP30 Expression Is Related With EMT in the Kidneys of Two Siberian Tigers With CKD
Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved..
BACKGROUND/AIM: Chronic kidney disease (CKD) is one of the most common causes of mortality in wild non-domestic felidae. The molecular mechanism regulating renal fibrosis in nephropathy is not fully understood especially in the felidae. This study aimed to elucidate senescence marker protein 30 (SMP30) expression patterns and its relationship with epithelial-mesenchymal transition (EMT) by immunostaining in two necropsied Siberian tigers (Panthera tigris altaica) with CKD.
MATERIALS AND METHODS: Two kidney samples from male Siberian tigers were fixed and tissue sections were stained for histopathological assay.
RESULTS: In CKD, renal tubular epithelial cells lost their tubular structures surrounded by severe interstitial fibrosis and were detached from the basement membrane. These damaged cells resembled the morphology of mesenchymal cells and showed much lower SMP30 expression compared with intact tubular epithelial cells. These cells also expressed vimentin, which is specifically expressed by mesenchymal cells, and through double staining, it was observed that vimentin was expressed in the tubular epithelial cells where SMP30 was not expressed. In addition, double-positive expression of pan-cytokeratin (pan-CK) and vimentin was found in damaged epithelial cells with mesenchymal features.
CONCLUSION: We demonstrated possible evidence to understand the role of SMP30 as a new pivotal factor and the possibility of decreased SMP30 as a potential indicator of EMT at the end stage of CKD.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
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| Enthalten in: |
In vivo (Athens, Greece) - 38(2024), 1 vom: 24. Jan., Seite 226-234 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jung, Yi-Rang [VerfasserIn] |
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| Links: |
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| Themen: |
Chronic kidney disease |
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| Anmerkungen: |
Date Completed 28.12.2023 Date Revised 04.01.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.21873/invivo.13429 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366387197 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. | ||
| 520 | |a BACKGROUND/AIM: Chronic kidney disease (CKD) is one of the most common causes of mortality in wild non-domestic felidae. The molecular mechanism regulating renal fibrosis in nephropathy is not fully understood especially in the felidae. This study aimed to elucidate senescence marker protein 30 (SMP30) expression patterns and its relationship with epithelial-mesenchymal transition (EMT) by immunostaining in two necropsied Siberian tigers (Panthera tigris altaica) with CKD | ||
| 520 | |a MATERIALS AND METHODS: Two kidney samples from male Siberian tigers were fixed and tissue sections were stained for histopathological assay | ||
| 520 | |a RESULTS: In CKD, renal tubular epithelial cells lost their tubular structures surrounded by severe interstitial fibrosis and were detached from the basement membrane. These damaged cells resembled the morphology of mesenchymal cells and showed much lower SMP30 expression compared with intact tubular epithelial cells. These cells also expressed vimentin, which is specifically expressed by mesenchymal cells, and through double staining, it was observed that vimentin was expressed in the tubular epithelial cells where SMP30 was not expressed. In addition, double-positive expression of pan-cytokeratin (pan-CK) and vimentin was found in damaged epithelial cells with mesenchymal features | ||
| 520 | |a CONCLUSION: We demonstrated possible evidence to understand the role of SMP30 as a new pivotal factor and the possibility of decreased SMP30 as a potential indicator of EMT at the end stage of CKD | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chronic kidney disease | |
| 650 | 4 | |a Siberian tiger | |
| 650 | 4 | |a epithelial–mesenchymal transition | |
| 650 | 4 | |a senescence marker protein 30 | |
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| 700 | 1 | |a Yim, Jae-Hyuk |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Young-Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sae-Bom |e verfasserin |4 aut | |
| 700 | 1 | |a Heo, Sung-Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Bae, Seul-Gi |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Kyoo-Tae |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Young-Sam |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Sang-Joon |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Jin-Kyu |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Tae-Hwan |e verfasserin |4 aut | |
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