Thrombospondin 1 and Reelin act through Vldlr to regulate cardiac growth and repair
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany..
Adult mammalian cardiomyocytes have minimal cell cycle capacity, which leads to poor regeneration after cardiac injury such as myocardial infarction. Many positive regulators of cardiomyocyte cell cycle and cardioprotective signals have been identified, but extracellular signals that suppress cardiomyocyte proliferation are poorly understood. We profiled receptors enriched in postnatal cardiomyocytes, and found that very-low-density-lipoprotein receptor (Vldlr) inhibits neonatal cardiomyocyte cell cycle. Paradoxically, Reelin, the well-known Vldlr ligand, expressed in cardiac Schwann cells and lymphatic endothelial cells, promotes neonatal cardiomyocyte proliferation. Thrombospondin1 (TSP-1), another ligand of Vldlr highly expressed in adult heart, was then found to inhibit cardiomyocyte proliferation through Vldlr, and may contribute to Vldlr's overall repression on proliferation. Mechanistically, Rac1 and subsequent Yap phosphorylation and nucleus translocation mediate the regulation of the cardiomyocyte cell cycle by TSP-1/Reelin-Vldlr signaling. Importantly, Reln mutant neonatal mice displayed impaired cardiomyocyte proliferation and cardiac regeneration after apical resection, while cardiac-specific Thbs1 deletion and cardiomyocyte-specific Vldlr deletion promote cardiomyocyte proliferation and are cardioprotective after myocardial infarction. Our results identified a novel role of Vldlr in consolidating extracellular signals to regulate cardiomyocyte cell cycle activity and survival, and the overall suppressive TSP-1-Vldlr signal may contribute to the poor cardiac repair capacity of adult mammals.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:119 |
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| Enthalten in: |
Basic research in cardiology - 119(2024), 1 vom: 01. Feb., Seite 169-192 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pei, Lijuan [VerfasserIn] |
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| Links: |
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| Themen: |
Cardiac protection |
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| Anmerkungen: |
Date Completed 12.02.2024 Date Revised 12.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00395-023-01021-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366378007 |
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| 245 | 1 | 0 | |a Thrombospondin 1 and Reelin act through Vldlr to regulate cardiac growth and repair |
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| 520 | |a © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany. | ||
| 520 | |a Adult mammalian cardiomyocytes have minimal cell cycle capacity, which leads to poor regeneration after cardiac injury such as myocardial infarction. Many positive regulators of cardiomyocyte cell cycle and cardioprotective signals have been identified, but extracellular signals that suppress cardiomyocyte proliferation are poorly understood. We profiled receptors enriched in postnatal cardiomyocytes, and found that very-low-density-lipoprotein receptor (Vldlr) inhibits neonatal cardiomyocyte cell cycle. Paradoxically, Reelin, the well-known Vldlr ligand, expressed in cardiac Schwann cells and lymphatic endothelial cells, promotes neonatal cardiomyocyte proliferation. Thrombospondin1 (TSP-1), another ligand of Vldlr highly expressed in adult heart, was then found to inhibit cardiomyocyte proliferation through Vldlr, and may contribute to Vldlr's overall repression on proliferation. Mechanistically, Rac1 and subsequent Yap phosphorylation and nucleus translocation mediate the regulation of the cardiomyocyte cell cycle by TSP-1/Reelin-Vldlr signaling. Importantly, Reln mutant neonatal mice displayed impaired cardiomyocyte proliferation and cardiac regeneration after apical resection, while cardiac-specific Thbs1 deletion and cardiomyocyte-specific Vldlr deletion promote cardiomyocyte proliferation and are cardioprotective after myocardial infarction. Our results identified a novel role of Vldlr in consolidating extracellular signals to regulate cardiomyocyte cell cycle activity and survival, and the overall suppressive TSP-1-Vldlr signal may contribute to the poor cardiac repair capacity of adult mammals | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cardiac protection | |
| 650 | 4 | |a Cardiomyocyte proliferation | |
| 650 | 4 | |a Reelin | |
| 650 | 4 | |a Thbs1 | |
| 650 | 4 | |a Vldlr | |
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| 650 | 7 | |a VLDL receptor |2 NLM | |
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| 700 | 1 | |a Ouyang, Zhaohui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hongjie |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Shiqi |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bilin |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Yansong |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Mengying |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Haocun |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Su |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Shuyue |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Zhao |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Dachun |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Guohua |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Ke |e verfasserin |4 aut | |
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