Evaluation of the accuracy of a multi-infection screening test based on a multiplex immunoassay targeting imported diseases common in migrant populations
Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved..
BACKGROUND: We aimed to evaluate the performance of a novel multiplex serological assay, able to simultaneously detect IgG of six infections, as a screening tool for imported diseases in migrants.
METHODS: Six panels of 40 (n = 240) anonymized serum samples with confirmed infections were used as positive controls to assess the multiplex assay's sensitivity. One panel of 40 sera from non-infected subjects was used to estimate the seropositivity cutoffs, and 32 non-infected sera were used as negative controls to estimate each serology's sensitivity and specificity. The multi-infection screening test was validated in a prospective cohort of 48 migrants from endemic areas. The sensitivity of the Luminex assay was calculated as the proportion of positive results over all positive samples identified by reference tests. The specificity was calculated using 32 negative samples. Uncertainty was quantified with 95 % confidence intervals using receiver operating characteristic analyses.
RESULTS: The sensitivity/specificity were 100 %/100 % for HIV (gp41 antigen), 97.5 %/100 % for Hepatitis B virus (HBV-core antigen), 100 %/100 % for Hepatitis C virus (HCV-core antigen), 92.5 %/90.6 % for strongyloidiasis [31-kDa recombinant antigen (NIE)], 97.5 %/100 % for schistosomiasis (combined serpin Schistosoma mansoni and S.haematobium antigens) and 95 %/90.6 % for Chagas disease [combined Trypanosoma cruzi kinetoplastid membrane protein-11 (KMP11) and paraflagellar rod proteins 2 (PFR2) antigens]. In the migrant cohort, antibody response to the combination of the T.cruzi antigens correctly identified 100 % individuals, whereas HBV-core antigen correctly identified 91.7 % and Strongyloides-NIE antigen 86.4 %.
CONCLUSIONS: We developed a new, robust and accurate 8-plex Luminex assay that could facilitate the implementation of screening programmes targeting migrant populations.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:57 |
|---|---|
| Enthalten in: |
Travel medicine and infectious disease - 57(2024) vom: 08. Jan., Seite 102681 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Aguilar, Ruth [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Chagas disease |
|---|
| Anmerkungen: |
Date Completed 22.01.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.tmaid.2023.102681 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366325612 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366325612 | ||
| 003 | DE-627 | ||
| 005 | 20240311232041.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231227s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.tmaid.2023.102681 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1323.xml |
| 035 | |a (DE-627)NLM366325612 | ||
| 035 | |a (NLM)38141899 | ||
| 035 | |a (PII)S1477-8939(23)00141-2 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Aguilar, Ruth |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluation of the accuracy of a multi-infection screening test based on a multiplex immunoassay targeting imported diseases common in migrant populations |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.01.2024 | ||
| 500 | |a Date Revised 11.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a BACKGROUND: We aimed to evaluate the performance of a novel multiplex serological assay, able to simultaneously detect IgG of six infections, as a screening tool for imported diseases in migrants | ||
| 520 | |a METHODS: Six panels of 40 (n = 240) anonymized serum samples with confirmed infections were used as positive controls to assess the multiplex assay's sensitivity. One panel of 40 sera from non-infected subjects was used to estimate the seropositivity cutoffs, and 32 non-infected sera were used as negative controls to estimate each serology's sensitivity and specificity. The multi-infection screening test was validated in a prospective cohort of 48 migrants from endemic areas. The sensitivity of the Luminex assay was calculated as the proportion of positive results over all positive samples identified by reference tests. The specificity was calculated using 32 negative samples. Uncertainty was quantified with 95 % confidence intervals using receiver operating characteristic analyses | ||
| 520 | |a RESULTS: The sensitivity/specificity were 100 %/100 % for HIV (gp41 antigen), 97.5 %/100 % for Hepatitis B virus (HBV-core antigen), 100 %/100 % for Hepatitis C virus (HCV-core antigen), 92.5 %/90.6 % for strongyloidiasis [31-kDa recombinant antigen (NIE)], 97.5 %/100 % for schistosomiasis (combined serpin Schistosoma mansoni and S.haematobium antigens) and 95 %/90.6 % for Chagas disease [combined Trypanosoma cruzi kinetoplastid membrane protein-11 (KMP11) and paraflagellar rod proteins 2 (PFR2) antigens]. In the migrant cohort, antibody response to the combination of the T.cruzi antigens correctly identified 100 % individuals, whereas HBV-core antigen correctly identified 91.7 % and Strongyloides-NIE antigen 86.4 % | ||
| 520 | |a CONCLUSIONS: We developed a new, robust and accurate 8-plex Luminex assay that could facilitate the implementation of screening programmes targeting migrant populations | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chagas disease | |
| 650 | 4 | |a Hepatitis C virus | |
| 650 | 4 | |a IgG | |
| 650 | 4 | |a Imported diseases | |
| 650 | 4 | |a Luminex | |
| 650 | 4 | |a Migrants | |
| 650 | 4 | |a Schistosomiasis | |
| 650 | 4 | |a Screening | |
| 650 | 4 | |a Sensitivity | |
| 650 | 4 | |a Specificity | |
| 650 | 4 | |a Strongyloidiasis | |
| 650 | 4 | |a Travel-related illnesses | |
| 700 | 1 | |a Cruz, Angeline |e verfasserin |4 aut | |
| 700 | 1 | |a Jiménez, Alfons |e verfasserin |4 aut | |
| 700 | 1 | |a Almuedo, Alex |e verfasserin |4 aut | |
| 700 | 1 | |a Saumell, Carme Roca |e verfasserin |4 aut | |
| 700 | 1 | |a Lopez, Marina Gigante |e verfasserin |4 aut | |
| 700 | 1 | |a Gasch, Oriol |e verfasserin |4 aut | |
| 700 | 1 | |a Falcó, Gemma |e verfasserin |4 aut | |
| 700 | 1 | |a Jiménez-Lozano, Ana |e verfasserin |4 aut | |
| 700 | 1 | |a Martínez-Perez, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Sanchez-Collado, Consol |e verfasserin |4 aut | |
| 700 | 1 | |a Tedesco, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a López, Manuel Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Pinazo, María Jesús |e verfasserin |4 aut | |
| 700 | 1 | |a Leonel, Thais |e verfasserin |4 aut | |
| 700 | 1 | |a Bisoffi, Zeno |e verfasserin |4 aut | |
| 700 | 1 | |a Färnert, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Dobaño, Carlota |e verfasserin |4 aut | |
| 700 | 1 | |a Requena-Méndez, Ana |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Travel medicine and infectious disease |d 2003 |g 57(2024) vom: 08. Jan., Seite 102681 |w (DE-627)NLM16457655X |x 1873-0442 |7 nnns |
| 773 | 1 | 8 | |g volume:57 |g year:2024 |g day:08 |g month:01 |g pages:102681 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.tmaid.2023.102681 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 57 |j 2024 |b 08 |c 01 |h 102681 | ||