Mycobacterium leprae is able to infect adipocytes, inducing lipolysis and modulating the immune response
Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved..
Leprosy is a chronic infectious disease caused by the intracellular bacillus Mycobacterium leprae (M. leprae), which is known to infect skin macrophages and Schwann cells. Although adipose tissue is a recognized site of Mycobacterium tuberculosis infection, its role in the histopathology of leprosy was, until now, unknown. We analyzed the M. leprae capacity to infect and persist inside adipocytes, characterizing the induction of a lipolytic phenotype in adipocytes, as well as the effect of these infected cells on macrophage recruitment. We evaluated 3T3-L1-derived adipocytes, inguinal adipose tissue of SWR/J mice, and subcutaneous adipose tissue biopsies of leprosy patients. M. leprae was able to infect 3T3-L1-derived adipocytes in vitro, presenting a strong lipolytic profile after infection, followed by significant cholesterol efflux. This lipolytic phenotype was replicated in vivo by M. leprae injection into mice inguinal adipose tissue. Furthermore, M. leprae was detected inside crown-like structures in the subcutaneous adipose tissue of multibacillary patients. These data indicate that subcutaneous adipose tissue could be an important site of infection, and probably persistence, for M. leprae, being involved in the modulation of the innate immune control in leprosy via the release of cholesterol, MCP-1, and adiponectin.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
Microbes and infection - 26(2024), 3 vom: 18. März, Seite 105283 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dos Reis, Sabrina Alves [VerfasserIn] |
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| Links: |
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| Themen: |
97C5T2UQ7J |
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| Anmerkungen: |
Date Completed 20.03.2024 Date Revised 20.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.micinf.2023.105283 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM366325140 |
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| 520 | |a Leprosy is a chronic infectious disease caused by the intracellular bacillus Mycobacterium leprae (M. leprae), which is known to infect skin macrophages and Schwann cells. Although adipose tissue is a recognized site of Mycobacterium tuberculosis infection, its role in the histopathology of leprosy was, until now, unknown. We analyzed the M. leprae capacity to infect and persist inside adipocytes, characterizing the induction of a lipolytic phenotype in adipocytes, as well as the effect of these infected cells on macrophage recruitment. We evaluated 3T3-L1-derived adipocytes, inguinal adipose tissue of SWR/J mice, and subcutaneous adipose tissue biopsies of leprosy patients. M. leprae was able to infect 3T3-L1-derived adipocytes in vitro, presenting a strong lipolytic profile after infection, followed by significant cholesterol efflux. This lipolytic phenotype was replicated in vivo by M. leprae injection into mice inguinal adipose tissue. Furthermore, M. leprae was detected inside crown-like structures in the subcutaneous adipose tissue of multibacillary patients. These data indicate that subcutaneous adipose tissue could be an important site of infection, and probably persistence, for M. leprae, being involved in the modulation of the innate immune control in leprosy via the release of cholesterol, MCP-1, and adiponectin | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Adiponectin | |
| 650 | 4 | |a CCL2 | |
| 650 | 4 | |a Leprosy | |
| 650 | 4 | |a MCP-1 | |
| 650 | 4 | |a Subcutaneous adipose tissue | |
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| 700 | 1 | |a Gonçalves, Jessica Dias |e verfasserin |4 aut | |
| 700 | 1 | |a Lima, Karoline Dos Anjos |e verfasserin |4 aut | |
| 700 | 1 | |a Demaria, Thaina Magalhaes |e verfasserin |4 aut | |
| 700 | 1 | |a Costa-Bartuli, Emylle |e verfasserin |4 aut | |
| 700 | 1 | |a Gomes, Tiago Araujo |e verfasserin |4 aut | |
| 700 | 1 | |a Corrêa, Meydson Benjamim Carvalho |e verfasserin |4 aut | |
| 700 | 1 | |a Atella, Georgia Correa |e verfasserin |4 aut | |
| 700 | 1 | |a Sola-Penna, Mauro |e verfasserin |4 aut | |
| 700 | 1 | |a Rosa, Patricia Sammarco |e verfasserin |4 aut | |
| 700 | 1 | |a Pessolani, Maria Cristina Vidal |e verfasserin |4 aut | |
| 700 | 1 | |a Nagajyothi, Jyothi |e verfasserin |4 aut | |
| 700 | 1 | |a Lara, Flavio Alves |e verfasserin |4 aut | |
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