Colorimetric detection for raloxifene based on Cu-PTs nanozyme with peroxidase-like activity
Copyright © 2023 Elsevier B.V. All rights reserved..
The amorphous Cu-containing phosphomolybdate (Cu-PTs) composite with high peroxidase (POD)-like activity at neutral conditions was explored as biosensors for raloxifene (RAF) detection. The strong attraction between negatively charged Cu-PTs and positively charged substrates 3,3',5,5'-tetramethylbenzidine (TMB), as well as the acceleration of the conversion of active Cu+/Cu2+ by the Cu/W bimetallic redox couples were demonstrated to play significant roles in POD-like activity in physiological environment. When RAF is presence, it can bind to the surface of Cu-PTs and changes the chemical signal on the material surface, leading to the decreased POD-like activity. Based on this, a colorimetric method was established for the sensitive assay of RAF with a lower limit of detection (LOD) of 0.025 mg/L and good recovery from 90.13% to 108.9%. This work paves a new way to design a POD-like colorimetric protocol for tracing RAF in pharmaceutical products and environmental samples.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:239 |
|---|---|
| Enthalten in: |
Journal of pharmaceutical and biomedical analysis - 239(2024) vom: 15. Jan., Seite 115922 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yang, Xiaolan [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 05.01.2024 Date Revised 05.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jpba.2023.115922 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366253646 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366253646 | ||
| 003 | DE-627 | ||
| 005 | 20240108142218.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231227s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jpba.2023.115922 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1249.xml |
| 035 | |a (DE-627)NLM366253646 | ||
| 035 | |a (NLM)38134706 | ||
| 035 | |a (PII)S0731-7085(23)00691-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yang, Xiaolan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Colorimetric detection for raloxifene based on Cu-PTs nanozyme with peroxidase-like activity |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 05.01.2024 | ||
| 500 | |a Date Revised 05.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a The amorphous Cu-containing phosphomolybdate (Cu-PTs) composite with high peroxidase (POD)-like activity at neutral conditions was explored as biosensors for raloxifene (RAF) detection. The strong attraction between negatively charged Cu-PTs and positively charged substrates 3,3',5,5'-tetramethylbenzidine (TMB), as well as the acceleration of the conversion of active Cu+/Cu2+ by the Cu/W bimetallic redox couples were demonstrated to play significant roles in POD-like activity in physiological environment. When RAF is presence, it can bind to the surface of Cu-PTs and changes the chemical signal on the material surface, leading to the decreased POD-like activity. Based on this, a colorimetric method was established for the sensitive assay of RAF with a lower limit of detection (LOD) of 0.025 mg/L and good recovery from 90.13% to 108.9%. This work paves a new way to design a POD-like colorimetric protocol for tracing RAF in pharmaceutical products and environmental samples | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Colorimetric assay | |
| 650 | 4 | |a Cu-containing phosphomolybdate | |
| 650 | 4 | |a Peroxidase-like activity | |
| 650 | 4 | |a Raloxifene | |
| 650 | 7 | |a Raloxifene Hydrochloride |2 NLM | |
| 650 | 7 | |a 4F86W47BR6 |2 NLM | |
| 650 | 7 | |a Peroxidase |2 NLM | |
| 650 | 7 | |a EC 1.11.1.7 |2 NLM | |
| 650 | 7 | |a Peroxidases |2 NLM | |
| 650 | 7 | |a EC 1.11.1.- |2 NLM | |
| 650 | 7 | |a Hydrogen Peroxide |2 NLM | |
| 650 | 7 | |a BBX060AN9V |2 NLM | |
| 700 | 1 | |a Li, Jitao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Qiulan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Dezhi |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Yaling |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of pharmaceutical and biomedical analysis |d 1983 |g 239(2024) vom: 15. Jan., Seite 115922 |w (DE-627)NLM012641928 |x 1873-264X |7 nnns |
| 773 | 1 | 8 | |g volume:239 |g year:2024 |g day:15 |g month:01 |g pages:115922 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jpba.2023.115922 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 239 |j 2024 |b 15 |c 01 |h 115922 | ||