Breakdown of hardly degradable carbohydrates (lignocellulose) in a two-stage anaerobic digestion plant is favored in the main fermenter
Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved..
The yield and productivity of biogas plants depend on the degradation performance of their microbiomes. The spatial separation of the anaerobic digestion (AD) process into a separate hydrolysis and a main fermenter should improve cultivation conditions of the microorganisms involved in the degradation of complex substrates like lignocellulosic biomass (LCB) and, thus, the performance of anaerobic digesters. However, relatively little is known about such two-stage processes. Here, we investigated the process performance of a two-stage agricultural AD over one year, focusing on chemical and technical process parameters and metagenome-centric metaproteomics. Technical and chemical parameters indicated stable operation of the main fermenter but varying conditions for the open hydrolysis fermenter. Matching this, the microbiome in the hydrolysis fermenter has a higher dynamic than in the main fermenter. Metaproteomics-based microbiome analysis revealed a partial separation between early and common steps in carbohydrate degradation and primary fermentation in the hydrolysis fermenter but complex carbohydrate degradation, secondary fermentation, and methanogenesis in the main fermenter. Detailed metagenomics and metaproteomics characterization of the single metagenome-assembled genomes showed that the species focus on specific substrate niches and do not utilize their full genetic potential to degrade, for example, LCB. Overall, it seems that a separation of AD in a hydrolysis and a main fermenter does not improve the cleavage of complex substrates but significantly improves the overall process performance. In contrast, the remaining methanogenic activity in the hydrolysis fermenter may cause methane losses.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:250 |
|---|---|
| Enthalten in: |
Water research - 250(2024) vom: 15. Jan., Seite 121020 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Heyer, Robert [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
11132-73-3 |
|---|
| Anmerkungen: |
Date Completed 23.01.2024 Date Revised 23.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.watres.2023.121020 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366189611 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366189611 | ||
| 003 | DE-627 | ||
| 005 | 20240123231952.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231227s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.watres.2023.121020 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1268.xml |
| 035 | |a (DE-627)NLM366189611 | ||
| 035 | |a (NLM)38128305 | ||
| 035 | |a (PII)S0043-1354(23)01460-4 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Heyer, Robert |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Breakdown of hardly degradable carbohydrates (lignocellulose) in a two-stage anaerobic digestion plant is favored in the main fermenter |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.01.2024 | ||
| 500 | |a Date Revised 23.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a The yield and productivity of biogas plants depend on the degradation performance of their microbiomes. The spatial separation of the anaerobic digestion (AD) process into a separate hydrolysis and a main fermenter should improve cultivation conditions of the microorganisms involved in the degradation of complex substrates like lignocellulosic biomass (LCB) and, thus, the performance of anaerobic digesters. However, relatively little is known about such two-stage processes. Here, we investigated the process performance of a two-stage agricultural AD over one year, focusing on chemical and technical process parameters and metagenome-centric metaproteomics. Technical and chemical parameters indicated stable operation of the main fermenter but varying conditions for the open hydrolysis fermenter. Matching this, the microbiome in the hydrolysis fermenter has a higher dynamic than in the main fermenter. Metaproteomics-based microbiome analysis revealed a partial separation between early and common steps in carbohydrate degradation and primary fermentation in the hydrolysis fermenter but complex carbohydrate degradation, secondary fermentation, and methanogenesis in the main fermenter. Detailed metagenomics and metaproteomics characterization of the single metagenome-assembled genomes showed that the species focus on specific substrate niches and do not utilize their full genetic potential to degrade, for example, LCB. Overall, it seems that a separation of AD in a hydrolysis and a main fermenter does not improve the cleavage of complex substrates but significantly improves the overall process performance. In contrast, the remaining methanogenic activity in the hydrolysis fermenter may cause methane losses | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biogas plant | |
| 650 | 4 | |a Host-phage interactions | |
| 650 | 4 | |a Hydrolysis fermenter | |
| 650 | 4 | |a Metaproteomics | |
| 650 | 4 | |a Microbiome | |
| 650 | 4 | |a Multi-omics | |
| 650 | 7 | |a lignocellulose |2 NLM | |
| 650 | 7 | |a 11132-73-3 |2 NLM | |
| 650 | 7 | |a Lignin |2 NLM | |
| 650 | 7 | |a 9005-53-2 |2 NLM | |
| 650 | 7 | |a Carbohydrates |2 NLM | |
| 650 | 7 | |a Methane |2 NLM | |
| 650 | 7 | |a OP0UW79H66 |2 NLM | |
| 700 | 1 | |a Hellwig, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Maus, Irena |e verfasserin |4 aut | |
| 700 | 1 | |a Walke, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Schlüter, Andreas |e verfasserin |4 aut | |
| 700 | 1 | |a Hassa, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Sczyrba, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Tubbesing, Tom |e verfasserin |4 aut | |
| 700 | 1 | |a Klocke, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Mächtig, Torsten |e verfasserin |4 aut | |
| 700 | 1 | |a Schallert, Kay |e verfasserin |4 aut | |
| 700 | 1 | |a Seick, Ingolf |e verfasserin |4 aut | |
| 700 | 1 | |a Reichl, Udo |e verfasserin |4 aut | |
| 700 | 1 | |a Benndorf, Dirk |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Water research |d 1990 |g 250(2024) vom: 15. Jan., Seite 121020 |w (DE-627)NLM111404762 |x 1879-2448 |7 nnns |
| 773 | 1 | 8 | |g volume:250 |g year:2024 |g day:15 |g month:01 |g pages:121020 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.watres.2023.121020 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 250 |j 2024 |b 15 |c 01 |h 121020 | ||