Cxcl1 monomer-dimer equilibrium controls neutrophil extravasation
© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Leukocyte Biology. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
The chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps including rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, which exists as both a monomer and a dimer, the trapped dimer caused faster rolling, less adhesion, and less extravasation. Whole-mount immunofluorescence of the cremaster and arrest assays confirmed these data. Moreover, the Cxcl1 dimer showed impaired LFA-1-mediated neutrophil arrest that could be attributed to impaired Cxcr2-mediated ERK signaling. We conclude that Cxcl1 monomer-dimer equilibrium and potent Cxcr2 activity of the monomer together coordinate the early events in neutrophil recruitment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:115 |
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| Enthalten in: |
Journal of leukocyte biology - 115(2024), 3 vom: 23. Feb., Seite 565-572 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
León-Vega, Iliana I [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.02.2024 Date Revised 05.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jleuko/qiad159 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM366187732 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of Society for Leukocyte Biology. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a The chemokine Cxcl1 plays a crucial role in recruiting neutrophils in response to infection. The early events in chemokine-mediated neutrophil extravasation involve a sequence of highly orchestrated steps including rolling, adhesion, arrest, and diapedesis. Cxcl1 function is determined by its properties of reversible monomer-dimer equilibrium and binding to Cxcr2 and glycosaminoglycans. Here, we characterized how these properties orchestrate extravasation using intravital microscopy of the cremaster. Compared to WT Cxcl1, which exists as both a monomer and a dimer, the trapped dimer caused faster rolling, less adhesion, and less extravasation. Whole-mount immunofluorescence of the cremaster and arrest assays confirmed these data. Moreover, the Cxcl1 dimer showed impaired LFA-1-mediated neutrophil arrest that could be attributed to impaired Cxcr2-mediated ERK signaling. We conclude that Cxcl1 monomer-dimer equilibrium and potent Cxcr2 activity of the monomer together coordinate the early events in neutrophil recruitment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Cxcl1 chemokine | |
| 650 | 4 | |a Cxcr2 receptor | |
| 650 | 4 | |a ERK signaling | |
| 650 | 4 | |a arrest | |
| 650 | 4 | |a diapedesis | |
| 650 | 4 | |a extravasation | |
| 650 | 4 | |a glycosaminoglycan | |
| 650 | 4 | |a keratinocyte-derived chemokine | |
| 650 | 4 | |a monomer–dimer equilibrium | |
| 650 | 7 | |a Chemokine CXCL1 |2 NLM | |
| 650 | 7 | |a Glycosaminoglycans |2 NLM | |
| 650 | 7 | |a Chemokines |2 NLM | |
| 650 | 7 | |a Receptors, Interleukin-8B |2 NLM | |
| 700 | 1 | |a Vadillo, Eduardo |e verfasserin |4 aut | |
| 700 | 1 | |a Vargas-Robles, Hilda |e verfasserin |4 aut | |
| 700 | 1 | |a Rajarathnam, Krishna |e verfasserin |4 aut | |
| 700 | 1 | |a Schnoor, Michael |e verfasserin |4 aut | |
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