Exosome-derived miR-5p-72106_14 in vascular endothelial cells regulates fate determination of BMSCs
Copyright © 2023 Elsevier Inc. All rights reserved..
Vascular endothelial cells have recently been shown to be associated with osteogenic activity. However, the mechanism of vascular endothelial cells promoting osteogenesis is unclear. Here, we found that exosomes secreted from human microvascular endothelial cells (HMEC-1) promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and inhibited adipogenic differentiation. Aged and ovariectomy mice treated with exosomes showed increased bone formation and decreased lipid accumulation in the bone marrow cavity. Additionally, we screened out novel exosomal miR-5p-72106_14 by miRNA-seq and confirmed that miR-5p-72106_14 promoted osteogenic differentiation and inhibited adipogenic differentiation of BMSCs by inhibiting STAT1. Our results suggest that vascular endothelial cell-derived exosomes are involved in BMSC differentiation and exosomal miR-5p-72106_14 is a major factor in regulating fate determination of BMSCs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:482 |
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| Enthalten in: |
Toxicology and applied pharmacology - 482(2024) vom: 01. Jan., Seite 116793 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Hang [VerfasserIn] |
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| Links: |
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| Themen: |
BMSCs |
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| Anmerkungen: |
Date Completed 17.01.2024 Date Revised 23.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.taap.2023.116793 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Vascular endothelial cells have recently been shown to be associated with osteogenic activity. However, the mechanism of vascular endothelial cells promoting osteogenesis is unclear. Here, we found that exosomes secreted from human microvascular endothelial cells (HMEC-1) promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and inhibited adipogenic differentiation. Aged and ovariectomy mice treated with exosomes showed increased bone formation and decreased lipid accumulation in the bone marrow cavity. Additionally, we screened out novel exosomal miR-5p-72106_14 by miRNA-seq and confirmed that miR-5p-72106_14 promoted osteogenic differentiation and inhibited adipogenic differentiation of BMSCs by inhibiting STAT1. Our results suggest that vascular endothelial cell-derived exosomes are involved in BMSC differentiation and exosomal miR-5p-72106_14 is a major factor in regulating fate determination of BMSCs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a BMSCs | |
| 650 | 4 | |a Exosomes | |
| 650 | 4 | |a STAT1 | |
| 650 | 4 | |a Vascular endothelial cells | |
| 650 | 4 | |a miR-5p-72106_14 | |
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| 700 | 1 | |a Wang, Jiaojiao |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Xinyan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Qiyue |e verfasserin |4 aut | |
| 700 | 1 | |a He, Jieyu |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Qiong |e verfasserin |4 aut | |
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