Neutrophil extracellular traps in rheumatoid arthritis and periodontitis : Contribution of PADI4 gene polymorphisms
© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd..
AIM: We sought to investigate the release of neutrophil extracellular traps (NETs) in neutrophils from individuals with rheumatoid arthritis (RA) and controls and compare the presence of NETs in gingival tissues according to periodontal status. Also, the association between single nucleotide polymorphisms (SNPs) of the peptidyl arginine deaminase type 4 (PADI4) gene and the GTG haplotype with RA, periodontitis and NETs was evaluated in vitro.
MATERIALS AND METHODS: Peripheral neutrophils were isolated by density gradient, and NET concentration was determined by the PicoGreen method. Immunofluorescence was studied to identify NETs by co-localization of myeloperoxidase (MPO)-citrullinated histone H3 (H3Cit). Genotyping for SNPs (PADI4_89; PADI4_90; PADI4_92; and PADI4_104) was performed in 87 individuals with RA and 111 controls.
RESULTS: The release of NETs in vitro was significantly higher in individuals with RA and periodontitis and when stimulated with Porphyromonas gingivalis. Gingival tissues from subjects with RA and periodontitis revealed increased numbers of MPO-H3Cit-positive cells. Individuals with the GTG haplotype showed a higher release of NETs in vitro and worse periodontal parameters.
CONCLUSIONS: The release of NETs by circulating neutrophils is associated with RA and periodontitis and is influenced by the presence of the GTG haplotype.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:51 |
|---|---|
| Enthalten in: |
Journal of clinical periodontology - 51(2024), 4 vom: 29. Apr., Seite 452-463 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Oliveira, Sicília Rezende [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 15.03.2024 Date Revised 03.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/jcpe.13921 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366064746 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366064746 | ||
| 003 | DE-627 | ||
| 005 | 20240403235131.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231227s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/jcpe.13921 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1363.xml |
| 035 | |a (DE-627)NLM366064746 | ||
| 035 | |a (NLM)38115803 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Oliveira, Sicília Rezende |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Neutrophil extracellular traps in rheumatoid arthritis and periodontitis |b Contribution of PADI4 gene polymorphisms |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 15.03.2024 | ||
| 500 | |a Date Revised 03.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. | ||
| 520 | |a AIM: We sought to investigate the release of neutrophil extracellular traps (NETs) in neutrophils from individuals with rheumatoid arthritis (RA) and controls and compare the presence of NETs in gingival tissues according to periodontal status. Also, the association between single nucleotide polymorphisms (SNPs) of the peptidyl arginine deaminase type 4 (PADI4) gene and the GTG haplotype with RA, periodontitis and NETs was evaluated in vitro | ||
| 520 | |a MATERIALS AND METHODS: Peripheral neutrophils were isolated by density gradient, and NET concentration was determined by the PicoGreen method. Immunofluorescence was studied to identify NETs by co-localization of myeloperoxidase (MPO)-citrullinated histone H3 (H3Cit). Genotyping for SNPs (PADI4_89; PADI4_90; PADI4_92; and PADI4_104) was performed in 87 individuals with RA and 111 controls | ||
| 520 | |a RESULTS: The release of NETs in vitro was significantly higher in individuals with RA and periodontitis and when stimulated with Porphyromonas gingivalis. Gingival tissues from subjects with RA and periodontitis revealed increased numbers of MPO-H3Cit-positive cells. Individuals with the GTG haplotype showed a higher release of NETs in vitro and worse periodontal parameters | ||
| 520 | |a CONCLUSIONS: The release of NETs by circulating neutrophils is associated with RA and periodontitis and is influenced by the presence of the GTG haplotype | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a neutrophil extracellular traps | |
| 650 | 4 | |a periodontitis | |
| 650 | 4 | |a protein-arginine deiminase type 4 | |
| 650 | 4 | |a rheumatoid arthritis | |
| 650 | 4 | |a single nucleotide polymorphism | |
| 650 | 7 | |a Protein-Arginine Deiminases |2 NLM | |
| 650 | 7 | |a EC 3.5.3.15 |2 NLM | |
| 700 | 1 | |a de Arruda, José Alcides Almeida |e verfasserin |4 aut | |
| 700 | 1 | |a Schneider, Ayda Henriques |e verfasserin |4 aut | |
| 700 | 1 | |a Bemquerer, Larissa Marques |e verfasserin |4 aut | |
| 700 | 1 | |a de Souza, Rayssa Maria Soalheiro |e verfasserin |4 aut | |
| 700 | 1 | |a Barbim, Paula |e verfasserin |4 aut | |
| 700 | 1 | |a de Mattos-Pereira, Gustavo Henrique |e verfasserin |4 aut | |
| 700 | 1 | |a Calderaro, Débora Cerqueira |e verfasserin |4 aut | |
| 700 | 1 | |a Machado, Caio Cavalcante |e verfasserin |4 aut | |
| 700 | 1 | |a Alves, Sandra Fukada |e verfasserin |4 aut | |
| 700 | 1 | |a Moreira, Paula Rocha |e verfasserin |4 aut | |
| 700 | 1 | |a de Oliveira, Renê Donizeti Ribeiro |e verfasserin |4 aut | |
| 700 | 1 | |a Louzada-Júnior, Paulo |e verfasserin |4 aut | |
| 700 | 1 | |a Abreu, Lucas Guimarães |e verfasserin |4 aut | |
| 700 | 1 | |a Cunha, Fernando Queiroz |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Tarcília Aparecida |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of clinical periodontology |d 1974 |g 51(2024), 4 vom: 29. Apr., Seite 452-463 |w (DE-627)NLM000276847 |x 1600-051X |7 nnns |
| 773 | 1 | 8 | |g volume:51 |g year:2024 |g number:4 |g day:29 |g month:04 |g pages:452-463 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/jcpe.13921 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 51 |j 2024 |e 4 |b 29 |c 04 |h 452-463 | ||