Comparison on anti-inflammatory activity of Gynostemma pentaphyllum processed with different methods
The aim of this study is to investigate the effects of Gynostemma pentaphyllum dried with two different methods(air drying and heating) on inflammation in acute lung injury(ALI) mice in vivo and in vitro. Lipopolysaccharide(LPS) was sprayed into the airway of wild type C57BL/6J male mice to establish the model, and the drug was injected into the tail vein 24 h after modeling. Lung function, lung tissue wet/dry weight(W/D) ratio, the total protein concentration, interleukin 6(IL-6), IL-1β, and tumor necrosis factor-α(TNF-α) in the bronchoalveolar lavage fluid(BALF), and pathological changes of the lung tissue were used to evaluate the effects of different gypenosides on ALI mice. The results showed that total gypenosides(YGGPs) and the gypenosides substituted with one or two glycosyl(GPs_(1-2)) in the air-dried sample improved the lung function, significantly lowered the levels of IL-1β and TNF-α in BALF, and alleviated the lung inflammation of ALI mice. Moreover, GPs_(1-2) had a more significant effect on inhibiting NO release in RAW264.7 cells. This study showed that different drying methods affected the anti-inflammatory activity of G. pentaphyllum, and the rare saponins in the air-dried sample without heating had better anti-inflammatory activity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 48(2023), 19 vom: 19. Okt., Seite 5235-5243 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Xu, Shu-Yang [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.12.2023 Date Revised 21.12.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.19540/j.cnki.cjcmm.20230711.303 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366047787 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The aim of this study is to investigate the effects of Gynostemma pentaphyllum dried with two different methods(air drying and heating) on inflammation in acute lung injury(ALI) mice in vivo and in vitro. Lipopolysaccharide(LPS) was sprayed into the airway of wild type C57BL/6J male mice to establish the model, and the drug was injected into the tail vein 24 h after modeling. Lung function, lung tissue wet/dry weight(W/D) ratio, the total protein concentration, interleukin 6(IL-6), IL-1β, and tumor necrosis factor-α(TNF-α) in the bronchoalveolar lavage fluid(BALF), and pathological changes of the lung tissue were used to evaluate the effects of different gypenosides on ALI mice. The results showed that total gypenosides(YGGPs) and the gypenosides substituted with one or two glycosyl(GPs_(1-2)) in the air-dried sample improved the lung function, significantly lowered the levels of IL-1β and TNF-α in BALF, and alleviated the lung inflammation of ALI mice. Moreover, GPs_(1-2) had a more significant effect on inhibiting NO release in RAW264.7 cells. This study showed that different drying methods affected the anti-inflammatory activity of G. pentaphyllum, and the rare saponins in the air-dried sample without heating had better anti-inflammatory activity | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a acute lung injury | |
| 650 | 4 | |a anti-inflammatory activity | |
| 650 | 4 | |a gypenosides | |
| 650 | 4 | |a processing method | |
| 650 | 4 | |a rare saponins | |
| 650 | 4 | |a sugar chain length | |
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| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 700 | 1 | |a Yang, Zi-Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Teng, Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xun-Jiang |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, De-Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Shou-Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zheng-Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Li-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Jing-Jing |e verfasserin |4 aut | |
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