Antimicrobial Potential of Rhynchophorus Beetle Larval Haemolymph Against Methicillin-Resistant Staphylococcus aureus and Neisseria gonorrhoeae
<b>Background and Objective:</b> The emergence of methicillin-resistant community-acquired <i>Staphylococcus aureus </i>and antibiotic-resistant <i>Neisseria gonorrhoeae</i> has raised significant concerns. Efforts to combat resistance involve the exploration of novel alternative therapies, particularly those derived from insect components. <i>Rhynchophorus</i> sp., a coconut pest commonly found in Southeast Asia, has haemolymph that exhibits bactericidal properties<i>.</i> The objective of this study was to assess the potential of the haemolymph of <i>Rhynchophorus</i> sp., larvae as an antimicrobial agent against Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Neisseria gonorrhoeae</i>. <b>Materials and Methods:</b> In this study, <i>Rhynchophorus</i> sp., larvae were gathered for the purpose of haemolymph extraction. These larvae were then divided into distinct groups, with one group subjected to immunization using <i>Escherichia coli</i>, while another group was left unimmunized. The study utilized the well diffusion method to evaluate antibacterial effectiveness. <b>Results:</b> Haemolymph fluid extracts from <i>Escherichia</i> coli-immunized <i>Rhynchophorus</i> sp., larvae, exhibited strong antibacterial activity, with an average value of 19.3±0.47 mm, against MRSA, more enhanced compared to unimmunized larvae. In contrast, haemolymph fluid extracts from <i>Escherichia coli</i>-immunized <i>Rhynchophorus</i> sp., larvae demonstrated a more moderate antibacterial activity, with a mean of 14.17±0.27 mm, against <i>Neisseria gonorrhoeae</i>, a level similar to unimmunized larvae. <b>Conclusion:</b> The haemolymph extracted from <i>Rhynchophorus </i>sp., beetles larvae exhibited antimicrobial effects against MRSA and <i>Neisseria gonorrhoeae</i>, particularly when it is enhanced through <i>Escherichia coli</i> immunization.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
Pakistan journal of biological sciences : PJBS - 26(2023), 10 vom: 18. Sept., Seite 543-548 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Niode, Nurdjannah Jane [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-Bacterial Agents |
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| Anmerkungen: |
Date Completed 20.12.2023 Date Revised 20.12.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.3923/pjbs.2023.543.548 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM366012592 |
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| 520 | |a <b>Background and Objective:</b> The emergence of methicillin-resistant community-acquired <i>Staphylococcus aureus </i>and antibiotic-resistant <i>Neisseria gonorrhoeae</i> has raised significant concerns. Efforts to combat resistance involve the exploration of novel alternative therapies, particularly those derived from insect components. <i>Rhynchophorus</i> sp., a coconut pest commonly found in Southeast Asia, has haemolymph that exhibits bactericidal properties<i>.</i> The objective of this study was to assess the potential of the haemolymph of <i>Rhynchophorus</i> sp., larvae as an antimicrobial agent against Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Neisseria gonorrhoeae</i>. <b>Materials and Methods:</b> In this study, <i>Rhynchophorus</i> sp., larvae were gathered for the purpose of haemolymph extraction. These larvae were then divided into distinct groups, with one group subjected to immunization using <i>Escherichia coli</i>, while another group was left unimmunized. The study utilized the well diffusion method to evaluate antibacterial effectiveness. <b>Results:</b> Haemolymph fluid extracts from <i>Escherichia</i> coli-immunized <i>Rhynchophorus</i> sp., larvae, exhibited strong antibacterial activity, with an average value of 19.3±0.47 mm, against MRSA, more enhanced compared to unimmunized larvae. In contrast, haemolymph fluid extracts from <i>Escherichia coli</i>-immunized <i>Rhynchophorus</i> sp., larvae demonstrated a more moderate antibacterial activity, with a mean of 14.17±0.27 mm, against <i>Neisseria gonorrhoeae</i>, a level similar to unimmunized larvae. <b>Conclusion:</b> The haemolymph extracted from <i>Rhynchophorus </i>sp., beetles larvae exhibited antimicrobial effects against MRSA and <i>Neisseria gonorrhoeae</i>, particularly when it is enhanced through <i>Escherichia coli</i> immunization | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Phoanda, Tilesky Caprizio |e verfasserin |4 aut | |
| 700 | 1 | |a Lukita, Stephanie |e verfasserin |4 aut | |
| 700 | 1 | |a Tallei, Trina Ekawati |e verfasserin |4 aut | |
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