Signal Amplification and Near-Infrared Translation of Enzymatic Reactions by Nanosensors
© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH..
Enzymatic reactions are used to detect analytes in a range of biochemical methods. To measure the presence of an analyte, the enzyme is conjugated to a recognition unit and converts a substrate into a (colored) product that is detectable by visible (VIS) light. Thus, the lowest enzymatic turnover that can be detected sets a limit on sensitivity. Here, we report that substrates and products of horseradish peroxidase (HRP) and β-galactosidase change the near-infrared (NIR) fluorescence of (bio)polymer modified single-walled carbon nanotubes (SWCNTs). They translate a VIS signal into a beneficial NIR signal. Moreover, the affinity of the nanosensors leads to a higher effective local concentration of the reactants. This causes a non-linear sensor-based signal amplification and translation (SENSAT). We find signal enhancement up to ≈120x for the HRP substrate p-phenylenediamine (PPD), which means that reactions below the limit of detection in the VIS can be followed in the NIR (≈1000 nm). The approach is also applicable to other substrates such as 3,3'-5,5'-tetramethylbenzidine (TMB). An adsorption-based theoretical model fits the observed signals and corroborates the sensor-based enhancement mechanism. This approach can be used to amplify signals, translate them into the NIR and increase sensitivity of biochemical assays.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
|---|---|
| Enthalten in: |
Angewandte Chemie (International ed. in English) - 63(2024), 9 vom: 26. Feb., Seite e202316965 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Metternich, Justus T [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Bioanalytical Assays |
|---|
| Anmerkungen: |
Date Completed 20.02.2024 Date Revised 20.02.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/anie.202316965 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM36590824X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM36590824X | ||
| 003 | DE-627 | ||
| 005 | 20240220232217.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231227s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/anie.202316965 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1300.xml |
| 035 | |a (DE-627)NLM36590824X | ||
| 035 | |a (NLM)38100133 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Metternich, Justus T |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Signal Amplification and Near-Infrared Translation of Enzymatic Reactions by Nanosensors |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.02.2024 | ||
| 500 | |a Date Revised 20.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH. | ||
| 520 | |a Enzymatic reactions are used to detect analytes in a range of biochemical methods. To measure the presence of an analyte, the enzyme is conjugated to a recognition unit and converts a substrate into a (colored) product that is detectable by visible (VIS) light. Thus, the lowest enzymatic turnover that can be detected sets a limit on sensitivity. Here, we report that substrates and products of horseradish peroxidase (HRP) and β-galactosidase change the near-infrared (NIR) fluorescence of (bio)polymer modified single-walled carbon nanotubes (SWCNTs). They translate a VIS signal into a beneficial NIR signal. Moreover, the affinity of the nanosensors leads to a higher effective local concentration of the reactants. This causes a non-linear sensor-based signal amplification and translation (SENSAT). We find signal enhancement up to ≈120x for the HRP substrate p-phenylenediamine (PPD), which means that reactions below the limit of detection in the VIS can be followed in the NIR (≈1000 nm). The approach is also applicable to other substrates such as 3,3'-5,5'-tetramethylbenzidine (TMB). An adsorption-based theoretical model fits the observed signals and corroborates the sensor-based enhancement mechanism. This approach can be used to amplify signals, translate them into the NIR and increase sensitivity of biochemical assays | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bioanalytical Assays | |
| 650 | 4 | |a Biosensors | |
| 650 | 4 | |a Carbon Nanotubes | |
| 650 | 4 | |a Enzymes | |
| 650 | 4 | |a Near-Infrared Fluorescence | |
| 650 | 7 | |a Nanotubes, Carbon |2 NLM | |
| 650 | 7 | |a Horseradish Peroxidase |2 NLM | |
| 650 | 7 | |a EC 1.11.1.- |2 NLM | |
| 700 | 1 | |a Hill, Björn |e verfasserin |4 aut | |
| 700 | 1 | |a Wartmann, Janus A C |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Kruskop, Rebecca M |e verfasserin |4 aut | |
| 700 | 1 | |a Neutsch, Krisztian |e verfasserin |4 aut | |
| 700 | 1 | |a Herbertz, Svenja |e verfasserin |4 aut | |
| 700 | 1 | |a Kruss, Sebastian |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Angewandte Chemie (International ed. in English) |d 1964 |g 63(2024), 9 vom: 26. Feb., Seite e202316965 |w (DE-627)NLM000105422 |x 1521-3773 |7 nnns |
| 773 | 1 | 8 | |g volume:63 |g year:2024 |g number:9 |g day:26 |g month:02 |g pages:e202316965 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/anie.202316965 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 63 |j 2024 |e 9 |b 26 |c 02 |h e202316965 | ||