Multipotent progenitors instruct ontogeny of the superior colliculus
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved..
The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and is composed of a rich diversity of excitatory and inhibitory neurons and glia. However, the developmental principles directing the generation of SC cell-type diversity are not understood. Here, we pursued systematic cell lineage tracing in silico and in vivo, preserving full spatial information, using genetic mosaic analysis with double markers (MADM)-based clonal analysis with single-cell sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron types, even at the stage of terminal division. While individual clonal units show no pre-defined cellular composition, the establishment of appropriate relative proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively, our findings provide an inaugural framework at the single-RGP/-cell level of the mammalian SC ontogeny.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:112 |
|---|---|
| Enthalten in: |
Neuron - 112(2024), 2 vom: 17. Jan., Seite 230-246.e11 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Cheung, Giselle [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Cell-type diversity |
|---|
| Anmerkungen: |
Date Completed 22.01.2024 Date Revised 22.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.neuron.2023.11.009 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM365875090 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM365875090 | ||
| 003 | DE-627 | ||
| 005 | 20240122232003.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231227s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.neuron.2023.11.009 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1267.xml |
| 035 | |a (DE-627)NLM365875090 | ||
| 035 | |a (NLM)38096816 | ||
| 035 | |a (PII)S0896-6273(23)00883-8 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Cheung, Giselle |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Multipotent progenitors instruct ontogeny of the superior colliculus |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.01.2024 | ||
| 500 | |a Date Revised 22.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and is composed of a rich diversity of excitatory and inhibitory neurons and glia. However, the developmental principles directing the generation of SC cell-type diversity are not understood. Here, we pursued systematic cell lineage tracing in silico and in vivo, preserving full spatial information, using genetic mosaic analysis with double markers (MADM)-based clonal analysis with single-cell sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron types, even at the stage of terminal division. While individual clonal units show no pre-defined cellular composition, the establishment of appropriate relative proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively, our findings provide an inaugural framework at the single-RGP/-cell level of the mammalian SC ontogeny | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a MADM | |
| 650 | 4 | |a MADM-CloneSeq | |
| 650 | 4 | |a RGP | |
| 650 | 4 | |a cell-type diversity | |
| 650 | 4 | |a clonal analysis | |
| 650 | 4 | |a lineage | |
| 650 | 4 | |a mosaic analysis with double markers | |
| 650 | 4 | |a radial glial progenitor | |
| 650 | 4 | |a stem cell | |
| 650 | 4 | |a superior colliculus | |
| 700 | 1 | |a Pauler, Florian M |e verfasserin |4 aut | |
| 700 | 1 | |a Koppensteiner, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Krausgruber, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Streicher, Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Schrammel, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Gutmann-Özgen, Natalie |e verfasserin |4 aut | |
| 700 | 1 | |a Ivec, Alexis E |e verfasserin |4 aut | |
| 700 | 1 | |a Bock, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Shigemoto, Ryuichi |e verfasserin |4 aut | |
| 700 | 1 | |a Hippenmeyer, Simon |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Neuron |d 1994 |g 112(2024), 2 vom: 17. Jan., Seite 230-246.e11 |w (DE-627)NLM012616826 |x 1097-4199 |7 nnns |
| 773 | 1 | 8 | |g volume:112 |g year:2024 |g number:2 |g day:17 |g month:01 |g pages:230-246.e11 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.neuron.2023.11.009 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 112 |j 2024 |e 2 |b 17 |c 01 |h 230-246.e11 | ||