Full-length sequence analysis of hepatitis C virus genotype 3b strains and development of an in vivo infectious 3b cDNA clone
IMPORTANCE: HCV genotype 3b is a difficult-to-treat subtype, associated with accelerated progression of liver disease and resistance to antivirals. Moreover, its prevalence has significantly increased among persons who inject drugs posing a serious risk of transmission in the general population. Thus, more genetic information and antiviral testing systems are required to develop novel therapeutic options for this genotype 3 subtype. We determined the complete genomic sequence and complexity of three genotype 3b isolates, which will be beneficial to study its biology and evolution. Furthermore, we developed a full-length in vivo infectious cDNA clone of genotype 3b and showed its robustness and genetic stability in human-liver chimeric mice. This is, to our knowledge the first reported infectious cDNA clone of HCV genotype 3b and will provide a valuable tool to evaluate antivirals and neutralizing antibodies in vivo, as well as in the development of infectious cell culture systems required for further research.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
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| Enthalten in: |
Journal of virology - (2023) vom: 21. Nov., Seite e0092523 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bajpai, Priyanka Shukla [VerfasserIn] |
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| Links: |
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| Themen: |
Genotype 3b |
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| Anmerkungen: |
Date Revised 13.12.2023 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1128/jvi.00925-23 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365832669 |
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| 520 | |a IMPORTANCE: HCV genotype 3b is a difficult-to-treat subtype, associated with accelerated progression of liver disease and resistance to antivirals. Moreover, its prevalence has significantly increased among persons who inject drugs posing a serious risk of transmission in the general population. Thus, more genetic information and antiviral testing systems are required to develop novel therapeutic options for this genotype 3 subtype. We determined the complete genomic sequence and complexity of three genotype 3b isolates, which will be beneficial to study its biology and evolution. Furthermore, we developed a full-length in vivo infectious cDNA clone of genotype 3b and showed its robustness and genetic stability in human-liver chimeric mice. This is, to our knowledge the first reported infectious cDNA clone of HCV genotype 3b and will provide a valuable tool to evaluate antivirals and neutralizing antibodies in vivo, as well as in the development of infectious cell culture systems required for further research | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HCV | |
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| 650 | 4 | |a human-liver chimeric mice | |
| 650 | 4 | |a in vivo infectious cDNA clone | |
| 650 | 4 | |a resistance-associated substitutions | |
| 700 | 1 | |a Collignon, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Sølund, Christina |e verfasserin |4 aut | |
| 700 | 1 | |a Madsen, Lone Wulff |e verfasserin |4 aut | |
| 700 | 1 | |a Christensen, Peer Brehm |e verfasserin |4 aut | |
| 700 | 1 | |a Øvrehus, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Weis, Nina |e verfasserin |4 aut | |
| 700 | 1 | |a Holmbeck, Kenn |e verfasserin |4 aut | |
| 700 | 1 | |a Fahnøe, Ulrik |e verfasserin |4 aut | |
| 700 | 1 | |a Bukh, Jens |e verfasserin |4 aut | |
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