Yacon root extract improves lipid metabolism in hyperlipidemic rats by inhibiting HMGCR expression and activating the PPARα/CYP7A1/CPT-1 pathway
OBJECTIVE: To investigate the effect of yacon root extract on lipid metabolism in rats with hyperlipidemia (HLP) and its underlying mechanisms.
METHODS: SD rat models of HLP induced by high- fat diet feeding for 8 weeks were randomized into the model group, fenofibrate treatment group (27 mg/kg), and yacon extract treatment groups at doses of 5, 2.5 and 1.25 g/kg (n=10). The rats were given corresponding drug treatments via gavage for 8 weeks. After the treatments, the rats were observed for body weight changes, liver coefficient, liver pathology, and serum levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). The mRNA and protein expressions of HMGCR, PPARα, CYP7A1, and CPT-1 in the liver were detected using RT-qPCR and Western blotting.
RESULTS: Compared with those in the model group, the rats treated with fenofibrate and 5 g/kg yacon root extract showed significantly slower body weight gain and lower liver coefficient (P < 0.05) with lower serum levels of TG, TC, and LDL- C (P < 0.05) but higher HDL- C level (P < 0.05). The HLP rat models showed obvious fatty degeneration and vacuolar changes in the liver, which were significantly alleviated by fenofibrate treatment and by treatment with yacon root extract in a dose-dependent manner. Both fenofibrate and 5 g/kg yacon root extract significantly lowered the mRNA and protein expression levels of HMGCR (P < 0.001) and increased the expressions of PPARα, CYP7A1, and CPT-1 in the liver of HLP rats (P < 0.001).
CONCLUSION: Yacon root extract can reduce serum TG and TC levels in HLP rats possibly by inhibiting HMGCR expression and activating the PPARα/CYP7A1/CPT-1 signaling pathway, thereby promoting fatty acid β oxidation and bile acid metabolism.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 43(2023), 11 vom: 20. Nov., Seite 1977-1983 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Gong, S [VerfasserIn] |
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| Links: |
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| Themen: |
English Abstract |
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| Anmerkungen: |
Date Revised 11.12.2023 published: Print Citation Status In-Process |
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| doi: |
10.12122/j.issn.1673-4254.2023.11.20 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365723843 |
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| 100 | 1 | |a Gong, S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Yacon root extract improves lipid metabolism in hyperlipidemic rats by inhibiting HMGCR expression and activating the PPARα/CYP7A1/CPT-1 pathway |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 11.12.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status In-Process | ||
| 520 | |a OBJECTIVE: To investigate the effect of yacon root extract on lipid metabolism in rats with hyperlipidemia (HLP) and its underlying mechanisms | ||
| 520 | |a METHODS: SD rat models of HLP induced by high- fat diet feeding for 8 weeks were randomized into the model group, fenofibrate treatment group (27 mg/kg), and yacon extract treatment groups at doses of 5, 2.5 and 1.25 g/kg (n=10). The rats were given corresponding drug treatments via gavage for 8 weeks. After the treatments, the rats were observed for body weight changes, liver coefficient, liver pathology, and serum levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). The mRNA and protein expressions of HMGCR, PPARα, CYP7A1, and CPT-1 in the liver were detected using RT-qPCR and Western blotting | ||
| 520 | |a RESULTS: Compared with those in the model group, the rats treated with fenofibrate and 5 g/kg yacon root extract showed significantly slower body weight gain and lower liver coefficient (P < 0.05) with lower serum levels of TG, TC, and LDL- C (P < 0.05) but higher HDL- C level (P < 0.05). The HLP rat models showed obvious fatty degeneration and vacuolar changes in the liver, which were significantly alleviated by fenofibrate treatment and by treatment with yacon root extract in a dose-dependent manner. Both fenofibrate and 5 g/kg yacon root extract significantly lowered the mRNA and protein expression levels of HMGCR (P < 0.001) and increased the expressions of PPARα, CYP7A1, and CPT-1 in the liver of HLP rats (P < 0.001) | ||
| 520 | |a CONCLUSION: Yacon root extract can reduce serum TG and TC levels in HLP rats possibly by inhibiting HMGCR expression and activating the PPARα/CYP7A1/CPT-1 signaling pathway, thereby promoting fatty acid β oxidation and bile acid metabolism | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HMGCR | |
| 650 | 4 | |a PPARα | |
| 650 | 4 | |a hyperlipidemia | |
| 650 | 4 | |a lipid metabolism | |
| 650 | 4 | |a yacon root extract | |
| 700 | 1 | |a Yang, J |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, J |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, X |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, S |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, G |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, N |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, J |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Z |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nan fang yi ke da xue xue bao = Journal of Southern Medical University |d 2006 |g 43(2023), 11 vom: 20. Nov., Seite 1977-1983 |w (DE-627)NLM16087646X |x 1673-4254 |7 nnns |
| 773 | 1 | 8 | |g volume:43 |g year:2023 |g number:11 |g day:20 |g month:11 |g pages:1977-1983 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.12122/j.issn.1673-4254.2023.11.20 |3 Volltext |
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