Expired Platelet Concentrate Up-Cycling : Growth Factor-Rich Bioproduct Preparation for FBS Substitute
Due to the short storage period, large quantities of platelet concentrate (PC) are expiring. The expired PC cannot be injected into a blood vessel, but the activity of bioactive molecules, especially growth factors, is still preserved. In this paper, we organized a process to obtain a growth factor-rich bioproduct for use as a supplement in human cell culture by optimizing freezing, thawing, and sterilization conditions. Each unit of PC displayed visual differences, diverse biochemical values, and growth factor concentrations. To minimize lot-to-lot variation, we pooled a minimum of 10 PC units. The concentrations of growth factors were maximized through five freeze-thaw cycles for 12 h at -80 °C for freezing and for 5 min at 36 °C for thawing. We used a cell strainer with 40 µm pores, followed by a 0.45 μm filter and a 0.22 μm filter sequentially to sterilize the bioproduct with minimizing loss. The obtained growth factors remained stable for 4-6 h at room temperature (23 °C), 24 h at 4 °C, and 12 months at -80 °C. Cellular responses to the growth factor-rich bioproduct were tested with primary human renal proximal tubule epithelial cells. The cells exhibited a significantly increased growth rate, compared to the fetal bovine serum (FBS)-treated control group. The cells maintained their characteristic cuboidal shape, and stem cells and renal progenitor cells also preserved their genetic characteristics during culture. Therefore, the growth factor-rich bioproduct isolated from expired PC through our process can be used as a medium supplement to replace FBS in human cell culture for clinical application.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Journal of clinical medicine - 12(2023), 23 vom: 27. Nov. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lee, Eun Hye [VerfasserIn] |
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| Links: |
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| Themen: |
Expired platelet concentrate (PC) |
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| Anmerkungen: |
Date Revised 09.12.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/jcm12237345 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Due to the short storage period, large quantities of platelet concentrate (PC) are expiring. The expired PC cannot be injected into a blood vessel, but the activity of bioactive molecules, especially growth factors, is still preserved. In this paper, we organized a process to obtain a growth factor-rich bioproduct for use as a supplement in human cell culture by optimizing freezing, thawing, and sterilization conditions. Each unit of PC displayed visual differences, diverse biochemical values, and growth factor concentrations. To minimize lot-to-lot variation, we pooled a minimum of 10 PC units. The concentrations of growth factors were maximized through five freeze-thaw cycles for 12 h at -80 °C for freezing and for 5 min at 36 °C for thawing. We used a cell strainer with 40 µm pores, followed by a 0.45 μm filter and a 0.22 μm filter sequentially to sterilize the bioproduct with minimizing loss. The obtained growth factors remained stable for 4-6 h at room temperature (23 °C), 24 h at 4 °C, and 12 months at -80 °C. Cellular responses to the growth factor-rich bioproduct were tested with primary human renal proximal tubule epithelial cells. The cells exhibited a significantly increased growth rate, compared to the fetal bovine serum (FBS)-treated control group. The cells maintained their characteristic cuboidal shape, and stem cells and renal progenitor cells also preserved their genetic characteristics during culture. Therefore, the growth factor-rich bioproduct isolated from expired PC through our process can be used as a medium supplement to replace FBS in human cell culture for clinical application | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a expired platelet concentrate (PC) | |
| 650 | 4 | |a fetal bovine serum (FBS) | |
| 650 | 4 | |a growth factors | |
| 650 | 4 | |a human stem cell culture | |
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| 700 | 1 | |a Chun, So Young |e verfasserin |4 aut | |
| 700 | 1 | |a Yoon, Bo Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Jeon, Minji |e verfasserin |4 aut | |
| 700 | 1 | |a Ha, Yun-Sok |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Jae-Wook |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Joonbeom |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Jeongshik |e verfasserin |4 aut | |
| 700 | 1 | |a Hwan Kim, Dae |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Sang-Joon |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Tae Gyun |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Bum Soo |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Hyun Tae |e verfasserin |4 aut | |
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