Ang1/Tie2/VE-Cadherin Signaling Regulates DPSCs in Vascular Maturation
Adding dental pulp stem cells (DPSCs) to vascular endothelial cell-formed vessel-like structures can increase the longevity of these vessel networks. DPSCs display pericyte-like cell functions and closely assemble endothelial cells (ECs). However, the mechanisms of DPSC-derived pericyte-like cells in stabilizing the vessel networks are not fully understood. In this study, we investigated the functions of E-DPSCs, which were DPSCs isolated from the direct coculture of human umbilical vein endothelial cells (HUVECs) and DPSCs, and T-DPSCs, which were DPSCs treated by transforming growth factor beta 1 (TGF-β1), in stabilizing blood vessels in vitro and in vivo. A 3-dimensional coculture spheroid sprouting assay was conducted to compare the functions of E-DPSCs and T-DPSCs in vitro. Dental pulp angiogenesis in the severe combined immunodeficiency (SCID) mouse model was used to explore the roles of E-DPSCs and T-DPSCs in vascularization in vivo. The results demonstrated that both E-DPSCs and T-DPSCs possess smooth muscle cell-like cell properties, exhibiting higher expression of the mural cell-specific markers and the suppression of HUVEC sprouting. E-DPSCs and T-DPSCs inhibited HUVEC sprouting by activating TEK tyrosine kinase (Tie2) signaling, upregulating vascular endothelial (VE)-cadherin, and downregulating vascular endothelial growth factor receptor 2 (VEGFR2). In vivo study revealed more perfused and total blood vessels in the HUVEC + E-DPSC group, HUVEC + T-DPSC group, angiopoietin 1 (Ang1) pretreated group, and vascular endothelial protein tyrosine phosphatase (VE-PTP) inhibitor pretreated group, compared to HUVEC + DPSC group. In conclusion, these data indicated that E-DPSCs and T-DPSCs could stabilize the newly formed blood vessels and accelerate their perfusion. The critical regulating pathways are Ang1/Tie2/VE-cadherin and VEGF/VEGFR2 signaling.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:103 |
---|---|
Enthalten in: |
Journal of dental research - 103(2024), 1 vom: 05. Jan., Seite 101-110 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhang, Y [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 22.12.2023 Date Revised 21.03.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1177/00220345231210227 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM365490393 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM365490393 | ||
003 | DE-627 | ||
005 | 20240321235704.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1177/00220345231210227 |2 doi | |
028 | 5 | 2 | |a pubmed24n1338.xml |
035 | |a (DE-627)NLM365490393 | ||
035 | |a (NLM)38058134 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhang, Y |e verfasserin |4 aut | |
245 | 1 | 0 | |a Ang1/Tie2/VE-Cadherin Signaling Regulates DPSCs in Vascular Maturation |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 22.12.2023 | ||
500 | |a Date Revised 21.03.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Adding dental pulp stem cells (DPSCs) to vascular endothelial cell-formed vessel-like structures can increase the longevity of these vessel networks. DPSCs display pericyte-like cell functions and closely assemble endothelial cells (ECs). However, the mechanisms of DPSC-derived pericyte-like cells in stabilizing the vessel networks are not fully understood. In this study, we investigated the functions of E-DPSCs, which were DPSCs isolated from the direct coculture of human umbilical vein endothelial cells (HUVECs) and DPSCs, and T-DPSCs, which were DPSCs treated by transforming growth factor beta 1 (TGF-β1), in stabilizing blood vessels in vitro and in vivo. A 3-dimensional coculture spheroid sprouting assay was conducted to compare the functions of E-DPSCs and T-DPSCs in vitro. Dental pulp angiogenesis in the severe combined immunodeficiency (SCID) mouse model was used to explore the roles of E-DPSCs and T-DPSCs in vascularization in vivo. The results demonstrated that both E-DPSCs and T-DPSCs possess smooth muscle cell-like cell properties, exhibiting higher expression of the mural cell-specific markers and the suppression of HUVEC sprouting. E-DPSCs and T-DPSCs inhibited HUVEC sprouting by activating TEK tyrosine kinase (Tie2) signaling, upregulating vascular endothelial (VE)-cadherin, and downregulating vascular endothelial growth factor receptor 2 (VEGFR2). In vivo study revealed more perfused and total blood vessels in the HUVEC + E-DPSC group, HUVEC + T-DPSC group, angiopoietin 1 (Ang1) pretreated group, and vascular endothelial protein tyrosine phosphatase (VE-PTP) inhibitor pretreated group, compared to HUVEC + DPSC group. In conclusion, these data indicated that E-DPSCs and T-DPSCs could stabilize the newly formed blood vessels and accelerate their perfusion. The critical regulating pathways are Ang1/Tie2/VE-cadherin and VEGF/VEGFR2 signaling | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a dental pulp | |
650 | 4 | |a human umbilical vein endothelial cells | |
650 | 4 | |a pericytes | |
650 | 4 | |a stem cells | |
650 | 4 | |a transforming growth factor beta 1 | |
650 | 4 | |a vascular endothelial growth factor receptor 2 | |
650 | 7 | |a cadherin 5 |2 NLM | |
650 | 7 | |a Vascular Endothelial Growth Factor A |2 NLM | |
650 | 7 | |a Angiopoietin-1 |2 NLM | |
650 | 7 | |a Cadherins |2 NLM | |
700 | 1 | |a Lin, S |e verfasserin |4 aut | |
700 | 1 | |a Liu, J |e verfasserin |4 aut | |
700 | 1 | |a Chen, Q |e verfasserin |4 aut | |
700 | 1 | |a Kang, J |e verfasserin |4 aut | |
700 | 1 | |a Zhong, J |e verfasserin |4 aut | |
700 | 1 | |a Hu, M |e verfasserin |4 aut | |
700 | 1 | |a Basabrain, M S |e verfasserin |4 aut | |
700 | 1 | |a Liang, Y |e verfasserin |4 aut | |
700 | 1 | |a Yuan, C |e verfasserin |4 aut | |
700 | 1 | |a Zhang, C |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of dental research |d 1945 |g 103(2024), 1 vom: 05. Jan., Seite 101-110 |w (DE-627)NLM000005630 |x 1544-0591 |7 nnns |
773 | 1 | 8 | |g volume:103 |g year:2024 |g number:1 |g day:05 |g month:01 |g pages:101-110 |
856 | 4 | 0 | |u http://dx.doi.org/10.1177/00220345231210227 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 103 |j 2024 |e 1 |b 05 |c 01 |h 101-110 |