Rapamycin Exacerbates Staphylococcus aureus Pneumonia by Inhibiting mTOR-RPS6 in Macrophages
© 2023 Yu et al..
Purpose: This study aimed to explore the effect of Rapamycin (Rapa) in Staphylococcus aureus (S. aureus) pneumonia and clarify its possible mechanism.
Methods: We investigated the effects of Rapa on S. aureus pneumonia in mouse models and in macrophages cultured in vitro. Two possible mechanisms were investigated: the mTOR-RPS6 pathway phosphorylation and phagocytosis. Furthermore, for the mechanism verification in vivo, mice with specific Mtor knockout in myeloid cells were constructed for pneumonia models.
Results: Rapa exacerbated S. aureus pneumonia in mouse models, promoting chemokines secretion and inflammatory cells infiltration in lung. In vitro, Rapa upregulated the secretion of chemokines and cytokines in macrophages induced by S. aureus. Mechanistically, the mTOR-ribosomal protein S6 (RPS6) pathway in macrophages was phosphorylated in response to S. aureus infection, and the inhibition of RPS6 phosphorylation upregulated the inflammation level. However, Rapa did not increase the phagocytic activity. Accordingly, mice with specific Mtor knockout in myeloid cells experienced more severe S. aureus pneumonia.
Conclusion: Rapa exacerbates S. aureus pneumonia by increasing the inflammatory levels of macrophages. Inhibition of mTOR-RPS6 pathway upregulates the expression of cytokines and chemokines in macrophages, thus increases inflammatory cells infiltration and exacerbates tissue damage.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Journal of inflammation research - 16(2023) vom: 30., Seite 5715-5728 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Fang-Yi [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Revised 07.12.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.2147/JIR.S434483 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365445282 |
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| 245 | 1 | 0 | |a Rapamycin Exacerbates Staphylococcus aureus Pneumonia by Inhibiting mTOR-RPS6 in Macrophages |
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| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2023 Yu et al. | ||
| 520 | |a Purpose: This study aimed to explore the effect of Rapamycin (Rapa) in Staphylococcus aureus (S. aureus) pneumonia and clarify its possible mechanism | ||
| 520 | |a Methods: We investigated the effects of Rapa on S. aureus pneumonia in mouse models and in macrophages cultured in vitro. Two possible mechanisms were investigated: the mTOR-RPS6 pathway phosphorylation and phagocytosis. Furthermore, for the mechanism verification in vivo, mice with specific Mtor knockout in myeloid cells were constructed for pneumonia models | ||
| 520 | |a Results: Rapa exacerbated S. aureus pneumonia in mouse models, promoting chemokines secretion and inflammatory cells infiltration in lung. In vitro, Rapa upregulated the secretion of chemokines and cytokines in macrophages induced by S. aureus. Mechanistically, the mTOR-ribosomal protein S6 (RPS6) pathway in macrophages was phosphorylated in response to S. aureus infection, and the inhibition of RPS6 phosphorylation upregulated the inflammation level. However, Rapa did not increase the phagocytic activity. Accordingly, mice with specific Mtor knockout in myeloid cells experienced more severe S. aureus pneumonia | ||
| 520 | |a Conclusion: Rapa exacerbates S. aureus pneumonia by increasing the inflammatory levels of macrophages. Inhibition of mTOR-RPS6 pathway upregulates the expression of cytokines and chemokines in macrophages, thus increases inflammatory cells infiltration and exacerbates tissue damage | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Staphylococcus aureus | |
| 650 | 4 | |a mTOR- ribosomal protein S6 signaling pathway | |
| 650 | 4 | |a macrophage | |
| 650 | 4 | |a pneumonia | |
| 650 | 4 | |a rapamycin | |
| 700 | 1 | |a Zheng, Kua |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yin-Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Shen-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Weng, Qing-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yan-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Miao |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Zhong-Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Lou, Jia-Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zhi-Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Ying, Song-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Hua-Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wen |e verfasserin |4 aut | |
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