Reverse metabolomics for the discovery of chemical structures from humans
© 2023. The Author(s)..
Determining the structure and phenotypic context of molecules detected in untargeted metabolomics experiments remains challenging. Here we present reverse metabolomics as a discovery strategy, whereby tandem mass spectrometry spectra acquired from newly synthesized compounds are searched for in public metabolomics datasets to uncover phenotypic associations. To demonstrate the concept, we broadly synthesized and explored multiple classes of metabolites in humans, including N-acyl amides, fatty acid esters of hydroxy fatty acids, bile acid esters and conjugated bile acids. Using repository-scale analysis1,2, we discovered that some conjugated bile acids are associated with inflammatory bowel disease (IBD). Validation using four distinct human IBD cohorts showed that cholic acids conjugated to Glu, Ile/Leu, Phe, Thr, Trp or Tyr are increased in Crohn's disease. Several of these compounds and related structures affected pathways associated with IBD, such as interferon-γ production in CD4+ T cells3 and agonism of the pregnane X receptor4. Culture of bacteria belonging to the Bifidobacterium, Clostridium and Enterococcus genera produced these bile amidates. Because searching repositories with tandem mass spectrometry spectra has only recently become possible, this reverse metabolomics approach can now be used as a general strategy to discover other molecules from human and animal ecosystems.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:626 |
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| Enthalten in: |
Nature - 626(2024), 7998 vom: 01. Feb., Seite 419-426 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gentry, Emily C [VerfasserIn] |
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| Links: |
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| Themen: |
Amides |
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| Anmerkungen: |
Date Completed 13.02.2024 Date Revised 09.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41586-023-06906-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Reverse metabolomics for the discovery of chemical structures from humans |
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| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Determining the structure and phenotypic context of molecules detected in untargeted metabolomics experiments remains challenging. Here we present reverse metabolomics as a discovery strategy, whereby tandem mass spectrometry spectra acquired from newly synthesized compounds are searched for in public metabolomics datasets to uncover phenotypic associations. To demonstrate the concept, we broadly synthesized and explored multiple classes of metabolites in humans, including N-acyl amides, fatty acid esters of hydroxy fatty acids, bile acid esters and conjugated bile acids. Using repository-scale analysis1,2, we discovered that some conjugated bile acids are associated with inflammatory bowel disease (IBD). Validation using four distinct human IBD cohorts showed that cholic acids conjugated to Glu, Ile/Leu, Phe, Thr, Trp or Tyr are increased in Crohn's disease. Several of these compounds and related structures affected pathways associated with IBD, such as interferon-γ production in CD4+ T cells3 and agonism of the pregnane X receptor4. Culture of bacteria belonging to the Bifidobacterium, Clostridium and Enterococcus genera produced these bile amidates. Because searching repositories with tandem mass spectrometry spectra has only recently become possible, this reverse metabolomics approach can now be used as a general strategy to discover other molecules from human and animal ecosystems | ||
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| 700 | 1 | |a Collins, Stephanie L |e verfasserin |4 aut | |
| 700 | 1 | |a Panitchpakdi, Morgan |e verfasserin |4 aut | |
| 700 | 1 | |a Belda-Ferre, Pedro |e verfasserin |4 aut | |
| 700 | 1 | |a Stewart, Allison K |e verfasserin |4 aut | |
| 700 | 1 | |a Carrillo Terrazas, Marvic |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Hsueh-Han |e verfasserin |4 aut | |
| 700 | 1 | |a Zuffa, Simone |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Avila-Pacheco, Julian |e verfasserin |4 aut | |
| 700 | 1 | |a Plichta, Damian R |e verfasserin |4 aut | |
| 700 | 1 | |a Aron, Allegra T |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mingxun |e verfasserin |4 aut | |
| 700 | 1 | |a Jarmusch, Alan K |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Fuhua |e verfasserin |4 aut | |
| 700 | 1 | |a Syrkin-Nikolau, Mashette |e verfasserin |4 aut | |
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| 700 | 1 | |a Dorrestein, Pieter C |e verfasserin |4 aut | |
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