Lysine-rich rice partially enhanced the growth and development of skeletal system with better skeletal microarchitecture in young rats
Copyright © 2023 Elsevier Inc. All rights reserved..
Rice is the primary staple food for half of the world's population but is low in lysine content. Previously, we developed transgenic rice with enhanced free lysine content in rice seeds (lysine-rich rice), which was shown safe for consumption and improved the growth in rats. However, the effects of lysine-rich rice on skeletal growth and development remained unknown. In this study, we hypothesized that lysine-rich rice improved skeletal growth and development in weaning rats. Male weaning Sprague-Dawley rats received lysine-rich rice (HFL) diet, wild-type rice (WT) diet, or wild-type rice with various contents of lysine supplementation diet for 70 days. Bone microarchitectures were examined by microcomputed tomography, bone strength was investigated by mechanical test, and dynamics of bone growth were examined by histomorphometric analysis. In addition, we explored the molecular mechanism of lysine and skeletal growth through biochemical testing of growth hormone, bone turnover marker, and amino acid content of rat serum analysis, as well as in a cell culture system. Results indicated that the HFL diet improved rats' bone growth, strength, and microarchitecture compared with the WT diet group. In addition, the HFL diet increased the serum essential amino acids, growth hormone (insulin-like growth factor-1), and bone formation marker concentrations. The cell culture model showed that lysine deficiency reduced insulin-like growth factor-1 and Osterix expression, Akt/mammalian target of rapamycin signaling, and matrix mineralization, and inhibited osteoblast differentiation associated with bone growth. Our findings showed that lysine-rich rice improved skeletal growth and development in weaning rats. A further increase of rice lysine content is highly desirable to fully optimize bone growth and development.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:121 |
|---|---|
| Enthalten in: |
Nutrition research (New York, N.Y.) - 121(2024) vom: 06. Jan., Seite 67-81 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Suen, Pui Kit [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
9002-72-6 |
|---|
| Anmerkungen: |
Date Completed 08.01.2024 Date Revised 08.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.nutres.2023.11.005 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM365344222 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM365344222 | ||
| 003 | DE-627 | ||
| 005 | 20240114232043.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.nutres.2023.11.005 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1252.xml |
| 035 | |a (DE-627)NLM365344222 | ||
| 035 | |a (NLM)38043437 | ||
| 035 | |a (PII)S0271-5317(23)00111-2 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Suen, Pui Kit |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lysine-rich rice partially enhanced the growth and development of skeletal system with better skeletal microarchitecture in young rats |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.01.2024 | ||
| 500 | |a Date Revised 08.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a Rice is the primary staple food for half of the world's population but is low in lysine content. Previously, we developed transgenic rice with enhanced free lysine content in rice seeds (lysine-rich rice), which was shown safe for consumption and improved the growth in rats. However, the effects of lysine-rich rice on skeletal growth and development remained unknown. In this study, we hypothesized that lysine-rich rice improved skeletal growth and development in weaning rats. Male weaning Sprague-Dawley rats received lysine-rich rice (HFL) diet, wild-type rice (WT) diet, or wild-type rice with various contents of lysine supplementation diet for 70 days. Bone microarchitectures were examined by microcomputed tomography, bone strength was investigated by mechanical test, and dynamics of bone growth were examined by histomorphometric analysis. In addition, we explored the molecular mechanism of lysine and skeletal growth through biochemical testing of growth hormone, bone turnover marker, and amino acid content of rat serum analysis, as well as in a cell culture system. Results indicated that the HFL diet improved rats' bone growth, strength, and microarchitecture compared with the WT diet group. In addition, the HFL diet increased the serum essential amino acids, growth hormone (insulin-like growth factor-1), and bone formation marker concentrations. The cell culture model showed that lysine deficiency reduced insulin-like growth factor-1 and Osterix expression, Akt/mammalian target of rapamycin signaling, and matrix mineralization, and inhibited osteoblast differentiation associated with bone growth. Our findings showed that lysine-rich rice improved skeletal growth and development in weaning rats. A further increase of rice lysine content is highly desirable to fully optimize bone growth and development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Lysine | |
| 650 | 4 | |a Lysine biofortification | |
| 650 | 4 | |a Lysine-rich rice | |
| 650 | 4 | |a Skeletal development | |
| 650 | 4 | |a Skeletal growth | |
| 650 | 7 | |a Lysine |2 NLM | |
| 650 | 7 | |a K3Z4F929H6 |2 NLM | |
| 650 | 7 | |a Growth Hormone |2 NLM | |
| 650 | 7 | |a 9002-72-6 |2 NLM | |
| 700 | 1 | |a Zheng, Lizhen |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Qing-Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Mak, Wan Sheung |e verfasserin |4 aut | |
| 700 | 1 | |a Pak, Wan Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Mo, Kit Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Man-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qiao-Quan |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Samuel Sai-Ming |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nutrition research (New York, N.Y.) |d 1993 |g 121(2024) vom: 06. Jan., Seite 67-81 |w (DE-627)NLM082781540 |x 1879-0739 |7 nnns |
| 773 | 1 | 8 | |g volume:121 |g year:2024 |g day:06 |g month:01 |g pages:67-81 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.nutres.2023.11.005 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 121 |j 2024 |b 06 |c 01 |h 67-81 | ||