Interfacial Assembly of Bacterial Microcompartment Shell Proteins in Aqueous Multiphase Systems
© 2023 The Authors. Small published by Wiley‐VCH GmbH..
Compartments are a fundamental feature of life, based variously on lipid membranes, protein shells, or biopolymer phase separation. Here, this combines self-assembling bacterial microcompartment (BMC) shell proteins and liquid-liquid phase separation (LLPS) to develop new forms of compartmentalization. It is found that BMC shell proteins assemble at the liquid-liquid interfaces between either 1) the dextran-rich droplets and PEG-rich continuous phase of a poly(ethyleneglycol)(PEG)/dextran aqueous two-phase system, or 2) the polypeptide-rich coacervate droplets and continuous dilute phase of a polylysine/polyaspartate complex coacervate system. Interfacial protein assemblies in the coacervate system are sensitive to the ratio of cationic to anionic polypeptides, consistent with electrostatically-driven assembly. In both systems, interfacial protein assembly competes with aggregation, with protein concentration and polycation availability impacting coating. These two LLPS systems are then combined to form a three-phase system wherein coacervate droplets are contained within dextran-rich phase droplets. Interfacial localization of BMC hexameric shell proteins is tunable in a three-phase system by changing the polyelectrolyte charge ratio. The tens-of-micron scale BMC shell protein-coated droplets introduced here can accommodate bioactive cargo such as enzymes or RNA and represent a new synthetic cell strategy for organizing biomimetic functionality.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Small (Weinheim an der Bergstrasse, Germany) - 20(2024), 15 vom: 12. Apr., Seite e2308390 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Abeysinghe, A A Dharani T [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial Proteins |
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| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/smll.202308390 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Compartments are a fundamental feature of life, based variously on lipid membranes, protein shells, or biopolymer phase separation. Here, this combines self-assembling bacterial microcompartment (BMC) shell proteins and liquid-liquid phase separation (LLPS) to develop new forms of compartmentalization. It is found that BMC shell proteins assemble at the liquid-liquid interfaces between either 1) the dextran-rich droplets and PEG-rich continuous phase of a poly(ethyleneglycol)(PEG)/dextran aqueous two-phase system, or 2) the polypeptide-rich coacervate droplets and continuous dilute phase of a polylysine/polyaspartate complex coacervate system. Interfacial protein assemblies in the coacervate system are sensitive to the ratio of cationic to anionic polypeptides, consistent with electrostatically-driven assembly. In both systems, interfacial protein assembly competes with aggregation, with protein concentration and polycation availability impacting coating. These two LLPS systems are then combined to form a three-phase system wherein coacervate droplets are contained within dextran-rich phase droplets. Interfacial localization of BMC hexameric shell proteins is tunable in a three-phase system by changing the polyelectrolyte charge ratio. The tens-of-micron scale BMC shell protein-coated droplets introduced here can accommodate bioactive cargo such as enzymes or RNA and represent a new synthetic cell strategy for organizing biomimetic functionality | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a bioreactor | |
| 650 | 4 | |a bottom‐up synthetic biology | |
| 650 | 4 | |a compartmentalization | |
| 650 | 4 | |a protocell | |
| 650 | 4 | |a self‐assembly | |
| 650 | 4 | |a synthetic cell | |
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| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 700 | 1 | |a Young, Eric J |e verfasserin |4 aut | |
| 700 | 1 | |a Rowland, Andrew T |e verfasserin |4 aut | |
| 700 | 1 | |a Dunshee, Lucas C |e verfasserin |4 aut | |
| 700 | 1 | |a Urandur, Sandeep |e verfasserin |4 aut | |
| 700 | 1 | |a Sullivan, Millicent O |e verfasserin |4 aut | |
| 700 | 1 | |a Kerfeld, Cheryl A |e verfasserin |4 aut | |
| 700 | 1 | |a Keating, Christine D |e verfasserin |4 aut | |
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