Liver histology and hepatic progenitor cell activity in pediatric acute liver failure : Implications for clinical outcome
© 2023 Wiley Periodicals LLC..
BACKGROUND: Hepatic progenitor cell (HPC) activity and regenerative process that follows pediatric acute liver failure (PALF) is still not well understood. This clinicopathological study was thus conducted with an aim to study the correlation of liver histology and HPC activity with outcomes in PALF.
METHODS: All PALF patients with available hepatic histological specimens were included and specimens were analyzed for hepatocyte loss, HPC activity [using cytokeratin (CK) 7, CK19, sex-determining region Y-related high mobility group box(SOX)9 and epithelial cell adhesion molecule (EpCAM)], hepatocyte proliferation (using Ki67), and hepatocyte senescence (using p53 and p21).
RESULTS: Ninety-four children were included: 22 (23.4%) survived with native liver (SNL) (i.e., the good outcome group) while rest (i.e., the poor outcome group) either died [33%, 35.1%] or received liver transplant (LT) [39%, 41.5%]. When compared to subjects with poor outcomes, those in the SNL group exhibited significantly less severe hepatocyte loss, fewer HPC/hpf, more proliferating hepatocytes, and less senescent hepatocytes (p < .05). Increasing severity of hepatocyte loss (adjusted OR: 9.95, 95% CI: 4.22-23.45, p < .001) was identified as an independent predictor of poor outcome. Eighty percent children with >50% native hepatocyte loss had poor outcome within 10 days of hospitalization.
CONCLUSION: In PALF, more severe hepatocyte loss, higher number of HPC activation, lesser number of proliferating hepatocytes, and greater number of senescent hepatocytes are associated with a poor outcome. Loss of >50% hepatocytes is an independent predictor of poor outcome in PALF.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Pediatric transplantation - 28(2024), 1 vom: 26. Feb., Seite e14662 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Panda, Kalpana [VerfasserIn] |
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| Links: |
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| Themen: |
Hepatic progenitor cells |
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| Anmerkungen: |
Date Completed 07.02.2024 Date Revised 28.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/petr.14662 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365279889 |
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| 245 | 1 | 0 | |a Liver histology and hepatic progenitor cell activity in pediatric acute liver failure |b Implications for clinical outcome |
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| 520 | |a © 2023 Wiley Periodicals LLC. | ||
| 520 | |a BACKGROUND: Hepatic progenitor cell (HPC) activity and regenerative process that follows pediatric acute liver failure (PALF) is still not well understood. This clinicopathological study was thus conducted with an aim to study the correlation of liver histology and HPC activity with outcomes in PALF | ||
| 520 | |a METHODS: All PALF patients with available hepatic histological specimens were included and specimens were analyzed for hepatocyte loss, HPC activity [using cytokeratin (CK) 7, CK19, sex-determining region Y-related high mobility group box(SOX)9 and epithelial cell adhesion molecule (EpCAM)], hepatocyte proliferation (using Ki67), and hepatocyte senescence (using p53 and p21) | ||
| 520 | |a RESULTS: Ninety-four children were included: 22 (23.4%) survived with native liver (SNL) (i.e., the good outcome group) while rest (i.e., the poor outcome group) either died [33%, 35.1%] or received liver transplant (LT) [39%, 41.5%]. When compared to subjects with poor outcomes, those in the SNL group exhibited significantly less severe hepatocyte loss, fewer HPC/hpf, more proliferating hepatocytes, and less senescent hepatocytes (p < .05). Increasing severity of hepatocyte loss (adjusted OR: 9.95, 95% CI: 4.22-23.45, p < .001) was identified as an independent predictor of poor outcome. Eighty percent children with >50% native hepatocyte loss had poor outcome within 10 days of hospitalization | ||
| 520 | |a CONCLUSION: In PALF, more severe hepatocyte loss, higher number of HPC activation, lesser number of proliferating hepatocytes, and greater number of senescent hepatocytes are associated with a poor outcome. Loss of >50% hepatocytes is an independent predictor of poor outcome in PALF | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a hepatic progenitor cells | |
| 650 | 4 | |a hepatocyte loss | |
| 650 | 4 | |a hepatocyte senescence | |
| 650 | 4 | |a pediatric acute liver failure | |
| 650 | 4 | |a regeneration in acute liver failure | |
| 700 | 1 | |a Sood, Vikrant |e verfasserin |4 aut | |
| 700 | 1 | |a Lal, Bikrant Bihari |e verfasserin |4 aut | |
| 700 | 1 | |a Khanna, Rajeev |e verfasserin |4 aut | |
| 700 | 1 | |a Rastogi, Archana |e verfasserin |4 aut | |
| 700 | 1 | |a Ramakrishna, Gayatri |e verfasserin |4 aut | |
| 700 | 1 | |a Alam, Seema |e verfasserin |4 aut | |
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