Chrysin restores the cardioprotective effect of ischemic preconditioning in diabetes-challenged rat heart
© 2023 Published by Elsevier Ltd..
Background: Ischemic preconditioning (IPC) is the utmost capable design to achieve protection over ischemia-reperfusion injury (I/R), but this phenomenon gets attenuated during various pathological conditions like diabetes. Chrysin exhibits cardioprotection in various experiments however, its therapeutic potential on IPC-mediated cardioprotection via PI3K-Akt-eNOS pathway in streptozotocin (STZ) triggered diabetes-challenged rat heart is yet to be assessed. For that reason, the experiment has been planned to investigate chrysin's effect on the cardioprotective action of IPC involving the PI3K-Akt-eNOS cascade in rat hearts challenged to diabetes.
Methods: The project was accomplished through means of absorbance studies for biochemical parameters, infarct size measurement (TTC stain) and coronary flow.
Results: The findings of the present study revealed that STZ drastically augmented the serum glucose level and the chrysin significantly reversed the IPC-stimulated increased coronary flow, nitrite release, and reduced LDH (lactate dehydrogenase), CK-MB (creatine kinase) activities as well as infarct size in diabetes-induced rat heart. Furthermore, chrysin also reversed the IPC-induced reduction in oxidative stress in an isolated Langendorff's perfused diabetic rat heart. Moreover, four episodes of preconditioning by either PI3K or eNOS inhibitor in chrysin-pretreated diabetic rat hearts significantly abolished the protective effect of chrysin.
Conclusion: Consequently, these observations suggested that chrysin increases the therapeutic efficiency of IPC in mitigating I/R injury via PI3K-Akt-eNOS signalling in diabetes-challenged rat hearts. Hence, chrysin could be a potential alternative option to IPC in diabetic rat hearts.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Heliyon - 9(2023), 11 vom: 14. Nov., Seite e22052 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Singh, Geetanjali [VerfasserIn] |
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| Links: |
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| Themen: |
Chrysin |
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| Anmerkungen: |
Date Revised 01.12.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.heliyon.2023.e22052 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM365188786 |
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| 520 | |a © 2023 Published by Elsevier Ltd. | ||
| 520 | |a Background: Ischemic preconditioning (IPC) is the utmost capable design to achieve protection over ischemia-reperfusion injury (I/R), but this phenomenon gets attenuated during various pathological conditions like diabetes. Chrysin exhibits cardioprotection in various experiments however, its therapeutic potential on IPC-mediated cardioprotection via PI3K-Akt-eNOS pathway in streptozotocin (STZ) triggered diabetes-challenged rat heart is yet to be assessed. For that reason, the experiment has been planned to investigate chrysin's effect on the cardioprotective action of IPC involving the PI3K-Akt-eNOS cascade in rat hearts challenged to diabetes | ||
| 520 | |a Methods: The project was accomplished through means of absorbance studies for biochemical parameters, infarct size measurement (TTC stain) and coronary flow | ||
| 520 | |a Results: The findings of the present study revealed that STZ drastically augmented the serum glucose level and the chrysin significantly reversed the IPC-stimulated increased coronary flow, nitrite release, and reduced LDH (lactate dehydrogenase), CK-MB (creatine kinase) activities as well as infarct size in diabetes-induced rat heart. Furthermore, chrysin also reversed the IPC-induced reduction in oxidative stress in an isolated Langendorff's perfused diabetic rat heart. Moreover, four episodes of preconditioning by either PI3K or eNOS inhibitor in chrysin-pretreated diabetic rat hearts significantly abolished the protective effect of chrysin | ||
| 520 | |a Conclusion: Consequently, these observations suggested that chrysin increases the therapeutic efficiency of IPC in mitigating I/R injury via PI3K-Akt-eNOS signalling in diabetes-challenged rat hearts. Hence, chrysin could be a potential alternative option to IPC in diabetic rat hearts | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chrysin | |
| 650 | 4 | |a Ischemia reperfusion injury | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a PI3K | |
| 650 | 4 | |a eNOS | |
| 700 | 1 | |a Varshney, Vibhav |e verfasserin |4 aut | |
| 700 | 1 | |a Goyal, Ahsas |e verfasserin |4 aut | |
| 700 | 1 | |a Ali, Nemat |e verfasserin |4 aut | |
| 700 | 1 | |a Iqbal, Muzaffar |e verfasserin |4 aut | |
| 700 | 1 | |a Kaur, Ishnoor |e verfasserin |4 aut | |
| 700 | 1 | |a Vargas-De-La-Cruz, Celia |e verfasserin |4 aut | |
| 700 | 1 | |a Behl, Tapan |e verfasserin |4 aut | |
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