Surveillance of SARS-CoV-2 immunogenicity : loss of immunodominant HLA-A*02-restricted epitopes that activate CD8+ T cells
Copyright © 2023 Lopes-Ribeiro, Oliveira, Retes, Barbosa-Stancioli, da Fonseca, Tsuji and Coelho-dos-Reis..
Introduction and methods: In this present work, coronavirus subfamilies and SARS-CoV-2 Variants of Concern (VOCs) were investigated for the presence of MHC-I immunodominant viral peptides using in silico and in vitro tools.
Results: In our results, HLA-A*02 haplotype showed the highest number of immunodominant epitopes but with the lowest combined prediction score. Furthermore, a decrease in combined prediction score was observed for HLA-A*02-restricted epitopes when the original strain was compared to the VOCs, indicating that the mutations on the VOCs are promoting escape from HLA-A2-mediated antigen presentation, which characterizes a immune evasion process. Additionally, epitope signature analysis revealed major immunogenic peptide loss for structural (S) and non-structural (ORF8) proteins of VOCs in comparison to the Wuhan sequence.
Discussion: These results may indicate that the antiviral CD8+ T-cell responses generated by original strains could not be sufficient for clearance of variants in either newly or reinfection with SARS-CoV-2. In contrast, N epitopes remain the most conserved and reactive peptides across SARS-CoV-2 VOCs. Overall, our data could contribute to the rational design and development of new vaccinal platforms to induce a broad cellular CD8+ T cell antiviral response, aiming at controlling viral transmission of future SARS-CoV-2 variants.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Frontiers in immunology - 14(2023) vom: 20., Seite 1229712 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lopes-Ribeiro, Ágata [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.12.2023 Date Revised 20.03.2024 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.3389/fimmu.2023.1229712 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM365136506 |
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| 245 | 1 | 0 | |a Surveillance of SARS-CoV-2 immunogenicity |b loss of immunodominant HLA-A*02-restricted epitopes that activate CD8+ T cells |
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| 500 | |a Date Revised 20.03.2024 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Lopes-Ribeiro, Oliveira, Retes, Barbosa-Stancioli, da Fonseca, Tsuji and Coelho-dos-Reis. | ||
| 520 | |a Introduction and methods: In this present work, coronavirus subfamilies and SARS-CoV-2 Variants of Concern (VOCs) were investigated for the presence of MHC-I immunodominant viral peptides using in silico and in vitro tools | ||
| 520 | |a Results: In our results, HLA-A*02 haplotype showed the highest number of immunodominant epitopes but with the lowest combined prediction score. Furthermore, a decrease in combined prediction score was observed for HLA-A*02-restricted epitopes when the original strain was compared to the VOCs, indicating that the mutations on the VOCs are promoting escape from HLA-A2-mediated antigen presentation, which characterizes a immune evasion process. Additionally, epitope signature analysis revealed major immunogenic peptide loss for structural (S) and non-structural (ORF8) proteins of VOCs in comparison to the Wuhan sequence | ||
| 520 | |a Discussion: These results may indicate that the antiviral CD8+ T-cell responses generated by original strains could not be sufficient for clearance of variants in either newly or reinfection with SARS-CoV-2. In contrast, N epitopes remain the most conserved and reactive peptides across SARS-CoV-2 VOCs. Overall, our data could contribute to the rational design and development of new vaccinal platforms to induce a broad cellular CD8+ T cell antiviral response, aiming at controlling viral transmission of future SARS-CoV-2 variants | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CD8+ T cells | |
| 650 | 4 | |a HLA-A2 | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a immunoinformatics | |
| 650 | 4 | |a peptide microarray | |
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| 700 | 1 | |a Oliveira, Patrícia de Melo |e verfasserin |4 aut | |
| 700 | 1 | |a Retes, Henrique Morais |e verfasserin |4 aut | |
| 700 | 1 | |a Barbosa-Stancioli, Edel Figueiredo |e verfasserin |4 aut | |
| 700 | 1 | |a da Fonseca, Flávio Guimarães |e verfasserin |4 aut | |
| 700 | 1 | |a Tsuji, Moriya |e verfasserin |4 aut | |
| 700 | 1 | |a Coelho-Dos-Reis, Jordana Grazziela Alves |e verfasserin |4 aut | |
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