Effects of Cross-linked Hyaluronic Acid in a Rat Model of Vestibular and Cochlear Toxicity
©Copyright 2023 by Turkish Otorhinolaryngology-Head and Neck Surgery Society / Turkish Archives of Otorhinolaryngology is published by Galenos Publishing House..
Objective: To investigate the effects of cross-linked hyaluronic acid (CLHA) in an experimental model of vestibulotoxicity and cochleotoxicity.
Methods: Twenty-eight female Wistar albino rats (200-250 g) were divided into four groups. Group A received 0.06 mL of 13.33 mg/mL gentamicin, Group B received 0.06 mL of CLHA, Group C received 0.03 mL of 26.66 mg/mL gentamicin and 0.03 mL CLHA, and Group D received 0.06 mL of 0.09% saline. All groups underwent auditory brainstem response testing at 4-32 kHz, signal-to-noise ratio of distortion product otoacoustic emission measurements at 1.5-8 kHz and vestibular tests on days 0,1,7,10. The rats were sacrificed, and their labyrinths were histologically assessed and scored.
Results: The hearing thresholds of Groups A and C were similar and significantly higher than those of the other groups at all frequencies, beginning from day 1. The vestibular and histological scores of Groups A and C were similar and significantly higher than those of the other groups from day 1. The audiological results, vestibular scores, and histological scores of Groups B and D were similar, except for a temporary middle ear effusion and hearing threshold shift in Group B. No significant deterioration was observed in the audiological, vestibular, and histological analyses of Groups B and D.
Conclusion: That both Group A and Group C similarly showed worsening audiological, vestibular, and histological tests suggests that CLHA did not alter the pharmacokinetics and histologic results of gentamicin.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Turkish archives of otorhinolaryngology - 61(2023), 3 vom: 15. Sept., Seite 124-133 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Erkoç, Erdal [VerfasserIn] |
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| Links: |
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| Themen: |
Animal research |
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| Anmerkungen: |
Date Revised 01.12.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.4274/tao.2023.2023-5-14 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365115541 |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a ©Copyright 2023 by Turkish Otorhinolaryngology-Head and Neck Surgery Society / Turkish Archives of Otorhinolaryngology is published by Galenos Publishing House. | ||
| 520 | |a Objective: To investigate the effects of cross-linked hyaluronic acid (CLHA) in an experimental model of vestibulotoxicity and cochleotoxicity | ||
| 520 | |a Methods: Twenty-eight female Wistar albino rats (200-250 g) were divided into four groups. Group A received 0.06 mL of 13.33 mg/mL gentamicin, Group B received 0.06 mL of CLHA, Group C received 0.03 mL of 26.66 mg/mL gentamicin and 0.03 mL CLHA, and Group D received 0.06 mL of 0.09% saline. All groups underwent auditory brainstem response testing at 4-32 kHz, signal-to-noise ratio of distortion product otoacoustic emission measurements at 1.5-8 kHz and vestibular tests on days 0,1,7,10. The rats were sacrificed, and their labyrinths were histologically assessed and scored | ||
| 520 | |a Results: The hearing thresholds of Groups A and C were similar and significantly higher than those of the other groups at all frequencies, beginning from day 1. The vestibular and histological scores of Groups A and C were similar and significantly higher than those of the other groups from day 1. The audiological results, vestibular scores, and histological scores of Groups B and D were similar, except for a temporary middle ear effusion and hearing threshold shift in Group B. No significant deterioration was observed in the audiological, vestibular, and histological analyses of Groups B and D | ||
| 520 | |a Conclusion: That both Group A and Group C similarly showed worsening audiological, vestibular, and histological tests suggests that CLHA did not alter the pharmacokinetics and histologic results of gentamicin | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gentamicin | |
| 650 | 4 | |a animal research | |
| 650 | 4 | |a audiology | |
| 650 | 4 | |a drug-related ototoxicity | |
| 650 | 4 | |a hearing loss | |
| 650 | 4 | |a hyaluronan | |
| 650 | 4 | |a vestibular function tests | |
| 700 | 1 | |a Çakır Çetin, Aslı |e verfasserin |4 aut | |
| 700 | 1 | |a Mungan Durankaya, Serpil |e verfasserin |4 aut | |
| 700 | 1 | |a Çilaker Mıçılı, Serap |e verfasserin |4 aut | |
| 700 | 1 | |a Keskinoğlu, Pembe |e verfasserin |4 aut | |
| 700 | 1 | |a Yılmaz, Osman |e verfasserin |4 aut | |
| 700 | 1 | |a Kırkım, Günay |e verfasserin |4 aut | |
| 700 | 1 | |a Güneri, Enis Alpin |e verfasserin |4 aut | |
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