Application of PHA surface binding proteins of alkali-tolerant Bacillus as surfactants
© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia..
Amphiphilic protein has lipophilic and hydrophilic domains, displaying the potential for development as a biosurfactant. The polyhydroxyalkanoate (PHA) surface binding protein derived from Bacillus is a type of protein that has not been studied for its emulsifying properties. In this study, PHA granule-associated protein (PhaP), PHA regulatory protein (PhaQ), and PHA synthase subunit (PhaR) derived from an alkali-tolerant PHA-producing Bacillus cereus HBL-AI were found and heterologously expressed in E. coli and purified to investigate their application as biosurfactants. It showed that the emulsification ability and stability of three amphiphilic proteins were higher than those of widely used chemical surfactants in diesel oil, vegetable oil, and lubricating oil. In particular, the PhaQ protein studied for the first time can form a stable emulsion layer in vegetable oil at a lower concentration (50 µg/mL), which greatly reduced the amount of protein used in emulsification. This clearly demonstrated that the PHA-binding protein of HBL-AI can be well applied as an environmentally friendly biosurfactants.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
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| Enthalten in: |
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology - 55(2024), 1 vom: 30. März, Seite 169-177 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fan, Xueyu [VerfasserIn] |
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| Links: |
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| Themen: |
Alkali-tolerant Bacillus |
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| Anmerkungen: |
Date Completed 11.03.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s42770-023-01176-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365105554 |
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| 520 | |a © 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia. | ||
| 520 | |a Amphiphilic protein has lipophilic and hydrophilic domains, displaying the potential for development as a biosurfactant. The polyhydroxyalkanoate (PHA) surface binding protein derived from Bacillus is a type of protein that has not been studied for its emulsifying properties. In this study, PHA granule-associated protein (PhaP), PHA regulatory protein (PhaQ), and PHA synthase subunit (PhaR) derived from an alkali-tolerant PHA-producing Bacillus cereus HBL-AI were found and heterologously expressed in E. coli and purified to investigate their application as biosurfactants. It showed that the emulsification ability and stability of three amphiphilic proteins were higher than those of widely used chemical surfactants in diesel oil, vegetable oil, and lubricating oil. In particular, the PhaQ protein studied for the first time can form a stable emulsion layer in vegetable oil at a lower concentration (50 µg/mL), which greatly reduced the amount of protein used in emulsification. This clearly demonstrated that the PHA-binding protein of HBL-AI can be well applied as an environmentally friendly biosurfactants | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Alkali-tolerant Bacillus | |
| 650 | 4 | |a Biosurfactant | |
| 650 | 4 | |a Emulsifying properties | |
| 650 | 4 | |a PHA-binding protein | |
| 650 | 7 | |a Polyhydroxyalkanoates |2 NLM | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
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| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 700 | 1 | |a Fu, Shuangqing |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Junpo |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Dexu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xinyue |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Honglei |e verfasserin |4 aut | |
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