Identification of recG genetic interactions in Escherichia coli by transposon sequencing
IMPORTANCE: DNA damage and subsequent DNA repair processes are mutagenic in nature and an important driver of evolution in prokaryotes, including antibiotic resistance development. Genetic screening approaches, such as transposon sequencing (Tn-seq), have provided important new insights into gene function and genetic relationships. Here, we employed Tn-seq to gain insight into the function of the recG gene, which renders Escherichia coli cells moderately sensitive to a variety of DNA-damaging agents when they are absent. The reported recG genetic interactions can be used in combination with future screens to aid in a more complete reconstruction of DNA repair pathways in bacteria.
| Errataetall: |
CommentIn: J Bacteriol. 2023 Dec 19;205(12):e0027223. - PMID 38018999 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:205 |
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| Enthalten in: |
Journal of bacteriology - 205(2023), 12 vom: 19. Dez., Seite e0018423 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bonde, Nina J [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.12.2023 Date Revised 08.03.2024 published: Print-Electronic Dryad: 10.5061/dryad.dz08kps2t CommentIn: J Bacteriol. 2023 Dec 19;205(12):e0027223. - PMID 38018999 Citation Status MEDLINE |
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| doi: |
10.1128/jb.00184-23 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365101591 |
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| 245 | 1 | 0 | |a Identification of recG genetic interactions in Escherichia coli by transposon sequencing |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Dryad: 10.5061/dryad.dz08kps2t | ||
| 500 | |a CommentIn: J Bacteriol. 2023 Dec 19;205(12):e0027223. - PMID 38018999 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a IMPORTANCE: DNA damage and subsequent DNA repair processes are mutagenic in nature and an important driver of evolution in prokaryotes, including antibiotic resistance development. Genetic screening approaches, such as transposon sequencing (Tn-seq), have provided important new insights into gene function and genetic relationships. Here, we employed Tn-seq to gain insight into the function of the recG gene, which renders Escherichia coli cells moderately sensitive to a variety of DNA-damaging agents when they are absent. The reported recG genetic interactions can be used in combination with future screens to aid in a more complete reconstruction of DNA repair pathways in bacteria | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a DNA repair | |
| 650 | 4 | |a Escherichia coli | |
| 650 | 4 | |a RecG | |
| 650 | 4 | |a transposon sequencing | |
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| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 700 | 1 | |a Wood, Elizabeth A |e verfasserin |4 aut | |
| 700 | 1 | |a Myers, Kevin S |e verfasserin |4 aut | |
| 700 | 1 | |a Place, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Keck, James L |e verfasserin |4 aut | |
| 700 | 1 | |a Cox, Michael M |e verfasserin |4 aut | |
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