Evaluation of two automated real-time PCR-based quantification methods for whole blood Epstein-Barr viral load
Copyright © 2023 Elsevier Inc. All rights reserved..
Quantification of EBV DNA is important in transplantation settings for the diagnosis of post-transplantation. We evaluated the performance of the AltoStar® EBV PCR Kit 1.5 on whole blood specimens: limit of detection, linearity, accuracy, and precision were determined using the WHO NIBSC 09/260 international standard. Results of 69 clinical samples were compared between the AltoStar® EBV PCR Kit 1.5 (altona Diagnostics) and the RealTime EBV assay (Abbott). The LoD of the AltoStar® Kit was 148 IU/mL and linearity was between 375 and 500000. A high concordance was found between nominal value of the NIBSC dilutions and the AltoStar EBV result. The total variation ranged from 2.2% to 9.6%. Out of 69 clinical samples tested, there was a high concordance between the 22 paired results within the overlapping linear ranges of both tests. The AltoStar® EBV assay is reliable and accurate for EBV viral load determination on whole blood samples.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:108 |
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| Enthalten in: |
Diagnostic microbiology and infectious disease - 108(2024), 2 vom: 01. Jan., Seite 116101 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vancutsem, Ellen [VerfasserIn] |
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| Links: |
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| Themen: |
AltoStar® |
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| Anmerkungen: |
Date Completed 22.01.2024 Date Revised 22.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.diagmicrobio.2023.116101 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Quantification of EBV DNA is important in transplantation settings for the diagnosis of post-transplantation. We evaluated the performance of the AltoStar® EBV PCR Kit 1.5 on whole blood specimens: limit of detection, linearity, accuracy, and precision were determined using the WHO NIBSC 09/260 international standard. Results of 69 clinical samples were compared between the AltoStar® EBV PCR Kit 1.5 (altona Diagnostics) and the RealTime EBV assay (Abbott). The LoD of the AltoStar® Kit was 148 IU/mL and linearity was between 375 and 500000. A high concordance was found between nominal value of the NIBSC dilutions and the AltoStar EBV result. The total variation ranged from 2.2% to 9.6%. Out of 69 clinical samples tested, there was a high concordance between the 22 paired results within the overlapping linear ranges of both tests. The AltoStar® EBV assay is reliable and accurate for EBV viral load determination on whole blood samples | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Epstein-Barr Virus | |
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| 700 | 1 | |a Soetens, Oriane |e verfasserin |4 aut | |
| 700 | 1 | |a Wautier, Magali |e verfasserin |4 aut | |
| 700 | 1 | |a Dördelmann, Corinna |e verfasserin |4 aut | |
| 700 | 1 | |a Piérard, Denis |e verfasserin |4 aut | |
| 700 | 1 | |a Wybo, Ingrid |e verfasserin |4 aut | |
| 700 | 1 | |a Demuyser, Thomas |e verfasserin |4 aut | |
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