Proximal and Distal Bronchioles Contribute to the Pathogenesis of Non-Cystic Fibrosis Bronchiectasis
Rationale: Non-cystic fibrosis bronchiectasis (NCFB) may originate in bronchiolar regions of the lung. Accordingly, there is a need to characterize the morphology and molecular characteristics of NCFB bronchioles. Objectives: Test the hypothesis that NCFB exhibits a major component of bronchiolar disease manifest by mucus plugging and ectasia. Methods: Morphologic criteria and region-specific epithelial gene expression, measured histologically and by RNA in situ hybridization and immunohistochemistry, identified proximal and distal bronchioles in excised NCFB lungs. RNA in situ hybridization and immunohistochemistry assessed bronchiolar mucus accumulation and mucin gene expression. CRISPR-Cas9-mediated IL-1R1 knockout in human bronchial epithelial cultures tested IL-1α and IL-1β contributions to mucin production. Spatial transcriptional profiling characterized NCFB distal bronchiolar gene expression. Measurements and Main Results: Bronchiolar perimeters and lumen areas per section area were increased in proximal, but not distal, bronchioles in NCFB versus control lungs, suggesting proximal bronchiolectasis. In NCFB, mucus plugging was observed in ectatic proximal bronchioles and associated nonectatic distal bronchioles in sections with disease. MUC5AC and MUC5B mucins were upregulated in NCFB proximal bronchioles, whereas MUC5B was selectively upregulated in distal bronchioles. Bronchiolar mucus plugs were populated by IL-1β-expressing macrophages. NCFB sterile sputum supernatants induced human bronchial epithelial MUC5B and MUC5AC expression that was >80% blocked by IL-1R1 ablation. Spatial transcriptional profiling identified upregulation of genes associated with secretory cells, hypoxia, interleukin pathways, and IL-1β-producing macrophages in mucus plugs and downregulation of epithelial ciliogenesis genes. Conclusions: NCFB exhibits distinctive proximal and distal bronchiolar disease. Both bronchiolar regions exhibit bronchiolar secretory cell features and mucus plugging but differ in mucin gene regulation and ectasia.
| Errataetall: |
CommentIn: Am J Respir Crit Care Med. 2024 Feb 15;209(4):347-349. - PMID 38190706 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:209 |
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| Enthalten in: |
American journal of respiratory and critical care medicine - 209(2024), 4 vom: 15. Feb., Seite 374-389 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Asakura, Takanori [VerfasserIn] |
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| Links: |
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| Themen: |
63231-63-0 |
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| Anmerkungen: |
Date Completed 16.02.2024 Date Revised 22.02.2024 published: Print CommentIn: Am J Respir Crit Care Med. 2024 Feb 15;209(4):347-349. - PMID 38190706 Citation Status MEDLINE |
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| doi: |
10.1164/rccm.202306-1093OC |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM365072060 |
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| 100 | 1 | |a Asakura, Takanori |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Proximal and Distal Bronchioles Contribute to the Pathogenesis of Non-Cystic Fibrosis Bronchiectasis |
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| 500 | |a Date Completed 16.02.2024 | ||
| 500 | |a Date Revised 22.02.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a CommentIn: Am J Respir Crit Care Med. 2024 Feb 15;209(4):347-349. - PMID 38190706 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Rationale: Non-cystic fibrosis bronchiectasis (NCFB) may originate in bronchiolar regions of the lung. Accordingly, there is a need to characterize the morphology and molecular characteristics of NCFB bronchioles. Objectives: Test the hypothesis that NCFB exhibits a major component of bronchiolar disease manifest by mucus plugging and ectasia. Methods: Morphologic criteria and region-specific epithelial gene expression, measured histologically and by RNA in situ hybridization and immunohistochemistry, identified proximal and distal bronchioles in excised NCFB lungs. RNA in situ hybridization and immunohistochemistry assessed bronchiolar mucus accumulation and mucin gene expression. CRISPR-Cas9-mediated IL-1R1 knockout in human bronchial epithelial cultures tested IL-1α and IL-1β contributions to mucin production. Spatial transcriptional profiling characterized NCFB distal bronchiolar gene expression. Measurements and Main Results: Bronchiolar perimeters and lumen areas per section area were increased in proximal, but not distal, bronchioles in NCFB versus control lungs, suggesting proximal bronchiolectasis. In NCFB, mucus plugging was observed in ectatic proximal bronchioles and associated nonectatic distal bronchioles in sections with disease. MUC5AC and MUC5B mucins were upregulated in NCFB proximal bronchioles, whereas MUC5B was selectively upregulated in distal bronchioles. Bronchiolar mucus plugs were populated by IL-1β-expressing macrophages. NCFB sterile sputum supernatants induced human bronchial epithelial MUC5B and MUC5AC expression that was >80% blocked by IL-1R1 ablation. Spatial transcriptional profiling identified upregulation of genes associated with secretory cells, hypoxia, interleukin pathways, and IL-1β-producing macrophages in mucus plugs and downregulation of epithelial ciliogenesis genes. Conclusions: NCFB exhibits distinctive proximal and distal bronchiolar disease. Both bronchiolar regions exhibit bronchiolar secretory cell features and mucus plugging but differ in mucin gene regulation and ectasia | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Okuda, Kenichi |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Gang |e verfasserin |4 aut | |
| 700 | 1 | |a Dang, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Kato, Takafumi |e verfasserin |4 aut | |
| 700 | 1 | |a Mikami, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Schworer, Stephen A |e verfasserin |4 aut | |
| 700 | 1 | |a Gilmore, Rodney C |e verfasserin |4 aut | |
| 700 | 1 | |a Radicioni, Giorgia |e verfasserin |4 aut | |
| 700 | 1 | |a Hawkins, Padraig |e verfasserin |4 aut | |
| 700 | 1 | |a Barbosa Cardenas, Selene Margarita |e verfasserin |4 aut | |
| 700 | 1 | |a Saito, Minako |e verfasserin |4 aut | |
| 700 | 1 | |a Cawley, Anne Marie |e verfasserin |4 aut | |
| 700 | 1 | |a De la Cruz, Gabriela |e verfasserin |4 aut | |
| 700 | 1 | |a Chua, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Alexis, Neil E |e verfasserin |4 aut | |
| 700 | 1 | |a Masugi, Yohei |e verfasserin |4 aut | |
| 700 | 1 | |a Noone, Peadar G |e verfasserin |4 aut | |
| 700 | 1 | |a Ribeiro, Carla M P |e verfasserin |4 aut | |
| 700 | 1 | |a Kesimer, Mehmet |e verfasserin |4 aut | |
| 700 | 1 | |a Olivier, Kenneth N |e verfasserin |4 aut | |
| 700 | 1 | |a Hasegawa, Naoki |e verfasserin |4 aut | |
| 700 | 1 | |a Randell, Scott H |e verfasserin |4 aut | |
| 700 | 1 | |a O'Neal, Wanda K |e verfasserin |4 aut | |
| 700 | 1 | |a Boucher, Richard C |e verfasserin |4 aut | |
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