A YTHDF-PABP interaction is required for m6 A-mediated organogenesis in plants
© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license..
N6-methyladenosine (m6 A) in mRNA is key to eukaryotic gene regulation. Many m6 A functions involve RNA-binding proteins that recognize m6 A via a YT521-B Homology (YTH) domain. YTH domain proteins contain long intrinsically disordered regions (IDRs) that may mediate phase separation and interaction with protein partners, but whose precise biochemical functions remain largely unknown. The Arabidopsis thaliana YTH domain proteins ECT2, ECT3, and ECT4 accelerate organogenesis through stimulation of cell division in organ primordia. Here, we use ECT2 to reveal molecular underpinnings of this function. We show that stimulation of leaf formation requires the long N-terminal IDR, and we identify two short IDR elements required for ECT2-mediated organogenesis. Of these two, a 19-amino acid region containing a tyrosine-rich motif conserved in both plant and metazoan YTHDF proteins is necessary for binding to the major cytoplasmic poly(A)-binding proteins PAB2, PAB4, and PAB8. Remarkably, overexpression of PAB4 in leaf primordia partially rescues the delayed leaf formation in ect2 ect3 ect4 mutants, suggesting that the ECT2-PAB2/4/8 interaction on target mRNAs of organogenesis-related genes may overcome limiting PAB concentrations in primordial cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
EMBO reports - 24(2023), 12 vom: 06. Dez., Seite e57741 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Due Tankmar, Mathias [VerfasserIn] |
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| Links: |
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| Themen: |
Arabidopsis |
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| Anmerkungen: |
Date Completed 11.12.2023 Date Revised 11.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.15252/embr.202357741 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM365007773 |
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| 245 | 1 | 2 | |a A YTHDF-PABP interaction is required for m6 A-mediated organogenesis in plants |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license. | ||
| 520 | |a N6-methyladenosine (m6 A) in mRNA is key to eukaryotic gene regulation. Many m6 A functions involve RNA-binding proteins that recognize m6 A via a YT521-B Homology (YTH) domain. YTH domain proteins contain long intrinsically disordered regions (IDRs) that may mediate phase separation and interaction with protein partners, but whose precise biochemical functions remain largely unknown. The Arabidopsis thaliana YTH domain proteins ECT2, ECT3, and ECT4 accelerate organogenesis through stimulation of cell division in organ primordia. Here, we use ECT2 to reveal molecular underpinnings of this function. We show that stimulation of leaf formation requires the long N-terminal IDR, and we identify two short IDR elements required for ECT2-mediated organogenesis. Of these two, a 19-amino acid region containing a tyrosine-rich motif conserved in both plant and metazoan YTHDF proteins is necessary for binding to the major cytoplasmic poly(A)-binding proteins PAB2, PAB4, and PAB8. Remarkably, overexpression of PAB4 in leaf primordia partially rescues the delayed leaf formation in ect2 ect3 ect4 mutants, suggesting that the ECT2-PAB2/4/8 interaction on target mRNAs of organogenesis-related genes may overcome limiting PAB concentrations in primordial cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Arabidopsis | |
| 650 | 4 | |a ECT2 | |
| 650 | 4 | |a IDR | |
| 650 | 4 | |a PABP | |
| 650 | 4 | |a m6A | |
| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
| 650 | 7 | |a RNA-Binding Proteins |2 NLM | |
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| 650 | 7 | |a ECT2 protein, Arabidopsis |2 NLM | |
| 650 | 7 | |a Intracellular Signaling Peptides and Proteins |2 NLM | |
| 700 | 1 | |a Reichel, Marlene |e verfasserin |4 aut | |
| 700 | 1 | |a Arribas-Hernández, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Brodersen, Peter |e verfasserin |4 aut | |
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