Hsp90 β is critical for the infection of severe fever with thrombocytopenia syndrome virus
Copyright © 2023 The Authors. Publishing services by Elsevier B.V. All rights reserved..
Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV) is an emerging disease in East Asia with a fatality rate of up to 30%. However, the viral-host interaction of SFTSV remains largely unknown. The heat-shock protein 90 (Hsp90) family consists of highly conserved chaperones that fold and remodel proteins and has a broad impact on the infection of many viruses. Here, we showed that Hsp90 is an important host factor involved in SFTSV infection. Hsp90 inhibitors significantly reduced SFTSV replication, viral protein expression, and the formation of inclusion bodies consisting of nonstructural proteins (NSs). Among viral proteins, NSs appeared to be the most reduced when Hsp90 inhibitors were used, and further analysis showed that their translation was affected. Co-immunoprecipitation of NSs with four isomers of Hsp90 showed that Hsp90 β specifically interacted with them. Knockdown of Hsp90 β expression also inhibited replication of SFTSV. These results suggest that Hsp90 β plays a critical role during SFTSV infection and could be a potential target for the development of drugs against SFTS.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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| Enthalten in: |
Virologica Sinica - 39(2024), 1 vom: 20. Feb., Seite 113-122 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Bo [VerfasserIn] |
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| Links: |
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| Themen: |
Heat-shock protein 90 |
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| Anmerkungen: |
Date Completed 19.02.2024 Date Revised 22.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virs.2023.11.008 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364995955 |
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| 520 | |a Copyright © 2023 The Authors. Publishing services by Elsevier B.V. All rights reserved. | ||
| 520 | |a Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV) is an emerging disease in East Asia with a fatality rate of up to 30%. However, the viral-host interaction of SFTSV remains largely unknown. The heat-shock protein 90 (Hsp90) family consists of highly conserved chaperones that fold and remodel proteins and has a broad impact on the infection of many viruses. Here, we showed that Hsp90 is an important host factor involved in SFTSV infection. Hsp90 inhibitors significantly reduced SFTSV replication, viral protein expression, and the formation of inclusion bodies consisting of nonstructural proteins (NSs). Among viral proteins, NSs appeared to be the most reduced when Hsp90 inhibitors were used, and further analysis showed that their translation was affected. Co-immunoprecipitation of NSs with four isomers of Hsp90 showed that Hsp90 β specifically interacted with them. Knockdown of Hsp90 β expression also inhibited replication of SFTSV. These results suggest that Hsp90 β plays a critical role during SFTSV infection and could be a potential target for the development of drugs against SFTS | ||
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| 650 | 4 | |a Host-virus interaction | |
| 650 | 4 | |a Hsp90 β | |
| 650 | 4 | |a Nonstructural protein | |
| 650 | 4 | |a Severe fever with thrombocytopenia syndrome virus (SFTSV) | |
| 700 | 1 | |a Zhang, Leike |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Zhihong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Manli |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jia |e verfasserin |4 aut | |
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