The Halophilic Bacterium Paracoccus haeundaensis for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources
The study objective was to evaluate the potential production of polyhydroxyalkanoates (PHAs), a biodegradable plastic material, by Paracoccus haeundaensis for which PHA production has never been reported. To identify the most effective nitrogen-limited culture conditions for PHAs production from this bacterium, batch fermentation using glucose concentrations ranging from 4 g l-1 to 20 g l-1 with a fixed ammonium concentration of 0.5 g l-1 was carried out at 30°C and pH 8.0. A glucose supplement of 12 g l-1 produced the highest PHA concentration (1.6 g l-1) and PHA content (0.63 g g-1) thereby identifying the optimal condition for PHA production from this bacterium. Gas chromatography-mass spectrometry analysis suggests that P. haeundaensis mostly produced copolymer types of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from glucose concentrations at 12 g l-1 or higher under the nitrogen-limited conditions. When several other single carbon sources were evaluated for the most efficient PHA production, fructose provided the highest biomass (2.8 g l-1), and PHAs (1.29 g l-1) concentrations. Results indicated that this bacterium mostly produced the copolymers P(3HB-co-3HV) from single carbon sources composing a range of 93-98% of 3-hydroxybutyrate and 2-7% of 3-hydroxyvalerate, whereas mannose-supplemented conditions produced the only homopolymer type of P(3HB). However, when propionic acid as a secondary carbon source were supplemented into the media, P. haeundaensis produced the copolymer P(3HB-co-3HV), composed of a 50% maximum monomeric unit of 3-hydroxyvaleric acid (3HV). However, as the concentration of propionic acid increased, cell biomass and PHAs concentrations substantially decreased due to cell toxicity.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
---|---|
Enthalten in: |
Journal of microbiology and biotechnology - 34(2024), 1 vom: 28. Jan., Seite 74-84 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Kim, Seon Min [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 30.01.2024 Date Revised 07.02.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.4014/jmb.2305.05025 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM36488519X |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM36488519X | ||
003 | DE-627 | ||
005 | 20240207232125.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.4014/jmb.2305.05025 |2 doi | |
028 | 5 | 2 | |a pubmed24n1283.xml |
035 | |a (DE-627)NLM36488519X | ||
035 | |a (NLM)37997264 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Kim, Seon Min |e verfasserin |4 aut | |
245 | 1 | 4 | |a The Halophilic Bacterium Paracoccus haeundaensis for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 30.01.2024 | ||
500 | |a Date Revised 07.02.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a The study objective was to evaluate the potential production of polyhydroxyalkanoates (PHAs), a biodegradable plastic material, by Paracoccus haeundaensis for which PHA production has never been reported. To identify the most effective nitrogen-limited culture conditions for PHAs production from this bacterium, batch fermentation using glucose concentrations ranging from 4 g l-1 to 20 g l-1 with a fixed ammonium concentration of 0.5 g l-1 was carried out at 30°C and pH 8.0. A glucose supplement of 12 g l-1 produced the highest PHA concentration (1.6 g l-1) and PHA content (0.63 g g-1) thereby identifying the optimal condition for PHA production from this bacterium. Gas chromatography-mass spectrometry analysis suggests that P. haeundaensis mostly produced copolymer types of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from glucose concentrations at 12 g l-1 or higher under the nitrogen-limited conditions. When several other single carbon sources were evaluated for the most efficient PHA production, fructose provided the highest biomass (2.8 g l-1), and PHAs (1.29 g l-1) concentrations. Results indicated that this bacterium mostly produced the copolymers P(3HB-co-3HV) from single carbon sources composing a range of 93-98% of 3-hydroxybutyrate and 2-7% of 3-hydroxyvalerate, whereas mannose-supplemented conditions produced the only homopolymer type of P(3HB). However, when propionic acid as a secondary carbon source were supplemented into the media, P. haeundaensis produced the copolymer P(3HB-co-3HV), composed of a 50% maximum monomeric unit of 3-hydroxyvaleric acid (3HV). However, as the concentration of propionic acid increased, cell biomass and PHAs concentrations substantially decreased due to cell toxicity | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Paracoccus haeundaensis | |
650 | 4 | |a batch fermentation | |
650 | 4 | |a nitrogen-limited conditions | |
650 | 4 | |a poly(3-hydroxybutyrate) | |
650 | 4 | |a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | |
650 | 4 | |a polyhydroxyalkanoates | |
650 | 7 | |a Polyesters |2 NLM | |
650 | 7 | |a propionic acid |2 NLM | |
650 | 7 | |a JHU490RVYR |2 NLM | |
650 | 7 | |a beta-hydroxyvaleric acid |2 NLM | |
650 | 7 | |a 10237-77-1 |2 NLM | |
650 | 7 | |a Carbon |2 NLM | |
650 | 7 | |a 7440-44-0 |2 NLM | |
650 | 7 | |a poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) |2 NLM | |
650 | 7 | |a Hydroxybutyrates |2 NLM | |
650 | 7 | |a Polyhydroxyalkanoates |2 NLM | |
650 | 7 | |a Glucose |2 NLM | |
650 | 7 | |a IY9XDZ35W2 |2 NLM | |
650 | 7 | |a Nitrogen |2 NLM | |
650 | 7 | |a N762921K75 |2 NLM | |
650 | 7 | |a Polyhydroxybutyrates |2 NLM | |
650 | 7 | |a Pentanoic Acids |2 NLM | |
650 | 7 | |a Propionates |2 NLM | |
700 | 1 | |a Lee, Hye In |e verfasserin |4 aut | |
700 | 1 | |a Nam, Seung Won |e verfasserin |4 aut | |
700 | 1 | |a Jin, Deok Hyeon |e verfasserin |4 aut | |
700 | 1 | |a Jeong, Gwi-Taek |e verfasserin |4 aut | |
700 | 1 | |a Nam, Soo-Wan |e verfasserin |4 aut | |
700 | 1 | |a Burns, Brendan |e verfasserin |4 aut | |
700 | 1 | |a Jeon, Young Jae |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of microbiology and biotechnology |d 1997 |g 34(2024), 1 vom: 28. Jan., Seite 74-84 |w (DE-627)NLM093752350 |x 1738-8872 |7 nnns |
773 | 1 | 8 | |g volume:34 |g year:2024 |g number:1 |g day:28 |g month:01 |g pages:74-84 |
856 | 4 | 0 | |u http://dx.doi.org/10.4014/jmb.2305.05025 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 34 |j 2024 |e 1 |b 28 |c 01 |h 74-84 |