A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation
Copyright © 2023 Elsevier Inc. All rights reserved..
Impaired gastrointestinal motility is associated with gut dysbiosis. Probiotics, such as Bifidobacteria, can improve this bowel disorder; however, efficacy is strain-dependent. We determine that a genetic factor, the abfA cluster governing arabinan utilization, in Bifidobacterium longum impacts treatment efficacy against functional constipation (FC). In mice with FC, B. longum, but not an abfA mutant, improved gastrointestinal transit time, an affect that was dependent upon dietary arabinan. abfA genes were identified in other commensal bacteria, whose effects in ameliorating murine FC were similarly abfA-dependent. In a double-blind, randomized, placebo-controlled clinical trial, supplementation with abfA-cluster-carrying B. longum, but not an abfA-deficient strain, enriched arabinan-utilization residents, increased beneficial metabolites, and improved FC symptoms. Across human cohorts, abfA-cluster abundance can predict FC, and transplantation of abfA cluster-enriched human microbiota to FC-induced germ-free mice improved gut motility. Collectively, these findings demonstrate a role for microbial abfA cluster in ameliorating FC, establishing principles for genomics-directed probiotic therapies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Cell host & microbe - 31(2023), 12 vom: 13. Dez., Seite 1989-2006.e8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Chengcheng [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.12.2023 Date Revised 16.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.chom.2023.10.011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364839821 |
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| 245 | 1 | 2 | |a A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation |
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| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a Impaired gastrointestinal motility is associated with gut dysbiosis. Probiotics, such as Bifidobacteria, can improve this bowel disorder; however, efficacy is strain-dependent. We determine that a genetic factor, the abfA cluster governing arabinan utilization, in Bifidobacterium longum impacts treatment efficacy against functional constipation (FC). In mice with FC, B. longum, but not an abfA mutant, improved gastrointestinal transit time, an affect that was dependent upon dietary arabinan. abfA genes were identified in other commensal bacteria, whose effects in ameliorating murine FC were similarly abfA-dependent. In a double-blind, randomized, placebo-controlled clinical trial, supplementation with abfA-cluster-carrying B. longum, but not an abfA-deficient strain, enriched arabinan-utilization residents, increased beneficial metabolites, and improved FC symptoms. Across human cohorts, abfA-cluster abundance can predict FC, and transplantation of abfA cluster-enriched human microbiota to FC-induced germ-free mice improved gut motility. Collectively, these findings demonstrate a role for microbial abfA cluster in ameliorating FC, establishing principles for genomics-directed probiotic therapies | ||
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| 700 | 1 | |a Yu, Leilei |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Chenchen |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Shuaiming |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yufeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shunhe |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Fengwei |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Yuzheng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jianxin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Liming |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Shi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jiachao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhai, Qixiao |e verfasserin |4 aut | |
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