Heat inactivation of SARS-CoV 2 enabled the measurement of salivary cortisol during COVID-19 pandemic
© 2023. The Author(s)..
BACKGROUND AND AIM: Salivary cortisol has become an essential tool in the management of cortisol-related disease. In 2020 the sudden outbreak of COVID-19 pandemic caused several concerns about the use of saliva, due to the risk of contamination, and a European consensus further discourage using salivary cortisol. To decrease infectious risk, we handled specimens by applying a heat treatment to inactivate viral particles, further evaluating the impact of the COVID-19 pandemic on the use of salivary cortisol in clinical practice.
MATERIAL AND METHODS: Saliva samples were exposed for 10 min at 70 °C, then cortisol was measured using LC-MS/MS. The number of salivary cortisol examinations from 2013 to 2022 was extracted from the local electronic database: those performed in 2019, 2020, and 2021 were analyzed and compared with the historical data.
RESULTS: During 2020 we observed a decrease of 408 (-20%) examinations (p = 0.05) compared to 2019; especially in salivary cortisol daily rhythm and salivary cortisol/cortisone ratio (respectively reduction of 47% and 88%, p = 0.003 and p = 0.001). Analyzing year 2021 compared with 2020 we reported an increase of 420 examinations (+20%, p = 0.01), with a complete recovery of salivary cortisol measurement (considering 2019: p = 0.71). Major differences were observed between morning salivary cortisol (-20%, p = 0.017), LNSC (-21%, p = 0.012) and salivary cortisol rhythm (-22%, p = 0.056). No Sars-Cov2 infections related to working exposure were reported among laboratory's employers.
CONCLUSIONS: We speculate that the adoption of an appropriate technique to inactivate viral particles in saliva specimens allowed the safety maintenance of salivary collections, also during the Sars-CoV-2 outbreak.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:83 |
---|---|
Enthalten in: |
Endocrine - 83(2024), 3 vom: 28. März, Seite 775-782 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Voltan, Giacomo [VerfasserIn] |
---|
Links: |
---|
Themen: |
COVID-19 |
---|
Anmerkungen: |
Date Completed 01.03.2024 Date Revised 17.03.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1007/s12020-023-03597-z |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM364829796 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM364829796 | ||
003 | DE-627 | ||
005 | 20240317232934.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1007/s12020-023-03597-z |2 doi | |
028 | 5 | 2 | |a pubmed24n1333.xml |
035 | |a (DE-627)NLM364829796 | ||
035 | |a (NLM)37991703 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Voltan, Giacomo |e verfasserin |4 aut | |
245 | 1 | 0 | |a Heat inactivation of SARS-CoV 2 enabled the measurement of salivary cortisol during COVID-19 pandemic |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 01.03.2024 | ||
500 | |a Date Revised 17.03.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2023. The Author(s). | ||
520 | |a BACKGROUND AND AIM: Salivary cortisol has become an essential tool in the management of cortisol-related disease. In 2020 the sudden outbreak of COVID-19 pandemic caused several concerns about the use of saliva, due to the risk of contamination, and a European consensus further discourage using salivary cortisol. To decrease infectious risk, we handled specimens by applying a heat treatment to inactivate viral particles, further evaluating the impact of the COVID-19 pandemic on the use of salivary cortisol in clinical practice | ||
520 | |a MATERIAL AND METHODS: Saliva samples were exposed for 10 min at 70 °C, then cortisol was measured using LC-MS/MS. The number of salivary cortisol examinations from 2013 to 2022 was extracted from the local electronic database: those performed in 2019, 2020, and 2021 were analyzed and compared with the historical data | ||
520 | |a RESULTS: During 2020 we observed a decrease of 408 (-20%) examinations (p = 0.05) compared to 2019; especially in salivary cortisol daily rhythm and salivary cortisol/cortisone ratio (respectively reduction of 47% and 88%, p = 0.003 and p = 0.001). Analyzing year 2021 compared with 2020 we reported an increase of 420 examinations (+20%, p = 0.01), with a complete recovery of salivary cortisol measurement (considering 2019: p = 0.71). Major differences were observed between morning salivary cortisol (-20%, p = 0.017), LNSC (-21%, p = 0.012) and salivary cortisol rhythm (-22%, p = 0.056). No Sars-Cov2 infections related to working exposure were reported among laboratory's employers | ||
520 | |a CONCLUSIONS: We speculate that the adoption of an appropriate technique to inactivate viral particles in saliva specimens allowed the safety maintenance of salivary collections, also during the Sars-CoV-2 outbreak | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a COVID-19 | |
650 | 4 | |a Cortisone | |
650 | 4 | |a Cushing | |
650 | 4 | |a Pandemic | |
650 | 4 | |a Salivary cortisol | |
650 | 4 | |a Sars-Cov2 | |
650 | 7 | |a Hydrocortisone |2 NLM | |
650 | 7 | |a WI4X0X7BPJ |2 NLM | |
650 | 7 | |a Cortisone |2 NLM | |
650 | 7 | |a V27W9254FZ |2 NLM | |
700 | 1 | |a Antonelli, Giorgia |e verfasserin |4 aut | |
700 | 1 | |a Mondin, Alessandro |e verfasserin |4 aut | |
700 | 1 | |a Tizianel, Irene |e verfasserin |4 aut | |
700 | 1 | |a Sabbadin, Chiara |e verfasserin |4 aut | |
700 | 1 | |a Barbot, Mattia |e verfasserin |4 aut | |
700 | 1 | |a Basso, Daniela |e verfasserin |4 aut | |
700 | 1 | |a Scaroni, Carla |e verfasserin |4 aut | |
700 | 1 | |a Ceccato, Filippo |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Endocrine |d 1995 |g 83(2024), 3 vom: 28. März, Seite 775-782 |w (DE-627)NLM091795540 |x 1559-0100 |7 nnns |
773 | 1 | 8 | |g volume:83 |g year:2024 |g number:3 |g day:28 |g month:03 |g pages:775-782 |
856 | 4 | 0 | |u http://dx.doi.org/10.1007/s12020-023-03597-z |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 83 |j 2024 |e 3 |b 28 |c 03 |h 775-782 |