Use of an on/off tetracycline riboswitch to control protein production in Komagataella phaffii
© 2023. The Author(s)..
The methylotrophic yeast Komagataella phaffii is one of the most important microbial platforms to produce recombinant proteins. Despite its importance in the context of industrial biotechnology, the use of synthetic biology approaches in K. phaffii is hampered by the fact that few genetic tools are available for precise control of gene expression in this system. In this work, we used an RNA aptamer activated by tetracycline to modulate protein production at the translational level. Using lacZ as gene reporter, we have demonstrated significant reduction of the heterologous protein upon addition of tetracycline. Furthermore, this genetic control device was applied for the control of Ku70p. This protein is involved in non-homologous recombination and the control of its production paves the way for the development of strains exhibiting higher rates of homologous recombination.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
AMB Express - 13(2023), 1 vom: 21. Nov., Seite 131 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vieira, Igor Patrick Vasconcelos [VerfasserIn] |
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| Links: |
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| Themen: |
Aptamer |
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| Anmerkungen: |
Date Revised 24.11.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1186/s13568-023-01637-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM364811315 |
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| 520 | |a © 2023. The Author(s). | ||
| 520 | |a The methylotrophic yeast Komagataella phaffii is one of the most important microbial platforms to produce recombinant proteins. Despite its importance in the context of industrial biotechnology, the use of synthetic biology approaches in K. phaffii is hampered by the fact that few genetic tools are available for precise control of gene expression in this system. In this work, we used an RNA aptamer activated by tetracycline to modulate protein production at the translational level. Using lacZ as gene reporter, we have demonstrated significant reduction of the heterologous protein upon addition of tetracycline. Furthermore, this genetic control device was applied for the control of Ku70p. This protein is involved in non-homologous recombination and the control of its production paves the way for the development of strains exhibiting higher rates of homologous recombination | ||
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| 700 | 1 | |a De Marco, Janice Lisboa |e verfasserin |4 aut | |
| 700 | 1 | |a de Moraes, Lidia Maria Pepe |e verfasserin |4 aut | |
| 700 | 1 | |a Torres, Fernando Araripe Gonçalves |e verfasserin |4 aut | |
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