Immobilization by 21 days of bed rest results in type II collagen degradation in healthy individuals

Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved..

OBJECTIVE: To investigate the effects of 21 days of bed rest immobilization (with and without exercise and nutrition interventions) on type II collagen biomarker concentrations in healthy individuals.

DESIGN: Twelve healthy male participants (age 34.2 ± 8.3 years; body mass index 22.4 ± 1.7 kg/m²) were exposed to 6 days ambulatory baseline data collection (BDC), 21 days head-down-tilt bed rest (HDT, CON) + interventions (HDT + resistive vibration exercise (2 times/week, 25 minutes): RVE; HDT + RVE + whey protein (0.6 g/kg body weight/day) and bicarbonate supplementation (90 mmol KHCO3/day: NeX), and 6 days of re-ambulation (R) in a cross-over designed study. The starting HDT condition was randomized (CON-RVE-NEX, RVE-NEX-CON, NEX-CON-RVE). Blood and urine samples were collected before, during, and after HDT. Serum concentrations (s) of CPII, C2C, C1,2C, and urinary concentrations (u) of CTX-II and Coll2-1NO2 were measured.

RESULTS: Twenty-one days of HDT resulted in increased sCPII (p < 0.001), sC2C (p < 0.001), and sC1,2C (p = 0.001) (highest increases: sCPII (+24.2% - HDT5), sC2C (+24.4% - HDT7), sC1,2C (+13.5% - HDT2). sC2C remained elevated at R+1 (p = 0.002) and R+6 (p < 0.001) compared to baseline. NeX led to lower sCPII (p < 0.001) and sC1,2C (p = 0.003) compared to CON. uCTX-II (second void and 24-hour urine) increased during HDT (p < 0.001, highest increase on HDT21: second void +82.8% (p < 0.001); 24-hour urine + 77.8% (p < 0.001). NeX resulted in lower uCTX-II concentrations in 24-hour urine (p = 0.012) compared to CON.

CONCLUSIONS: Twenty-one days of bed rest immobilization results in type II collagen degradation that does not recover within 6 days of resuming ambulation. The combination of resistive vibration exercise and protein/bicarbonate supplementation minimally counteracted this effect.

Medienart:

E-Artikel

Erscheinungsjahr:

2024

Erschienen:

2024

Enthalten in:

Zur Gesamtaufnahme - volume:32

Enthalten in:

Osteoarthritis and cartilage - 32(2024), 2 vom: 21. Jan., Seite 177-186

Sprache:

Englisch

Beteiligte Personen:

Liphardt, Anna-Maria [VerfasserIn]
Godonou, Elie-Tino [VerfasserIn]
Dreiner, Maren [VerfasserIn]
Mündermann, Annegret [VerfasserIn]
Tascilar, Koray [VerfasserIn]
Djalal, Nadja [VerfasserIn]
Heer, Martina [VerfasserIn]
Schett, Georg [VerfasserIn]
Zaucke, Frank [VerfasserIn]
Niehoff, Anja [VerfasserIn]

Links:

Volltext

Themen:

Bed rest
Bicarbonates
Cartilage biomarkers
Collagen Type II
Collagen metabolism
Immobilization
Journal Article
MNX-study
Type II collagen

Anmerkungen:

Date Completed 29.01.2024

Date Revised 29.01.2024

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.joca.2023.11.007

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM364807490