A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria
© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation..
Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of photosystem I (PSI) in cyanobacteria; however, how the BtpA stabilized PSI remains unexplained. To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (ΔbtpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only c. 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generated suppressor mutations that restore the photoautotrophy. Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) - the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the ΔbtpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR. Our results show that the BtpA protein is involved in the biogenesis of photosystems at the level of regulation of tetrapyrrole biosynthesis.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:241 |
|---|---|
| Enthalten in: |
The New phytologist - 241(2024), 3 vom: 20. Jan., Seite 1236-1249 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Skotnicová, Petra [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
1406-65-1 |
|---|
| Anmerkungen: |
Date Completed 12.01.2024 Date Revised 12.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/nph.19397 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM364774894 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM364774894 | ||
| 003 | DE-627 | ||
| 005 | 20240114234302.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/nph.19397 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1257.xml |
| 035 | |a (DE-627)NLM364774894 | ||
| 035 | |a (NLM)37986097 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Skotnicová, Petra |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 12.01.2024 | ||
| 500 | |a Date Revised 12.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation. | ||
| 520 | |a Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of photosystem I (PSI) in cyanobacteria; however, how the BtpA stabilized PSI remains unexplained. To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (ΔbtpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only c. 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generated suppressor mutations that restore the photoautotrophy. Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) - the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the ΔbtpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR. Our results show that the BtpA protein is involved in the biogenesis of photosystems at the level of regulation of tetrapyrrole biosynthesis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a BtpA | |
| 650 | 4 | |a Synechocystis sp. PCC 6803 | |
| 650 | 4 | |a cyanobacteria | |
| 650 | 4 | |a glutamyl-tRNA reductase | |
| 650 | 4 | |a tetrapyrrole biosynthesis | |
| 650 | 7 | |a Chlorophyll |2 NLM | |
| 650 | 7 | |a 1406-65-1 |2 NLM | |
| 650 | 7 | |a Photosystem I Protein Complex |2 NLM | |
| 650 | 7 | |a Tetrapyrroles |2 NLM | |
| 700 | 1 | |a Srivastava, Amit |e verfasserin |4 aut | |
| 700 | 1 | |a Aggarwal, Divya |e verfasserin |4 aut | |
| 700 | 1 | |a Talbot, Jana |e verfasserin |4 aut | |
| 700 | 1 | |a Karlínová, Iva |e verfasserin |4 aut | |
| 700 | 1 | |a Moos, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Mareš, Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Bučinská, Lenka |e verfasserin |4 aut | |
| 700 | 1 | |a Koník, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Šimek, Petr |e verfasserin |4 aut | |
| 700 | 1 | |a Tichý, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Sobotka, Roman |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The New phytologist |d 1979 |g 241(2024), 3 vom: 20. Jan., Seite 1236-1249 |w (DE-627)NLM09818248X |x 1469-8137 |7 nnns |
| 773 | 1 | 8 | |g volume:241 |g year:2024 |g number:3 |g day:20 |g month:01 |g pages:1236-1249 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/nph.19397 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 241 |j 2024 |e 3 |b 20 |c 01 |h 1236-1249 | ||