In vitro stability study of 10 beta-lactam antibiotics in human plasma samples
© 2023 Société Française de Pharmacologie et de Thérapeutique. Published by John Wiley & Sons Ltd..
BACKGROUND AND OBJECTIVES: Beta-lactam antibiotics are reported for some of them to be subject to a rapid degradation in infusion solutions and in human blood samples. However, the current data of stability available in blood samples are limited to a few number of beta-lactam antibiotics, and the methodology of the corresponding studies may be discussed. The objective of the present study is to evaluate the stability of 10 beta-lactam antibiotics in human plasma samples.
METHODS: Stability of amoxicillin, cefazolin, cefepime, cefotaxime, cefoxitin, ceftazidime, ceftriaxone, imipenem, meropenem, and piperacillin was evaluated at low and high concentrations at 20°C, 4°C, -20°C, and -80°C for 1, 7, 60, and 90 days, respectively.
RESULTS: Amoxicillin, cefepime, meropenem, and piperacillin were the least stable antibiotics. The maximum durations allowing the stability for all the evaluated beta-lactams at both tested concentrations were estimated at 3 h, 23 h, 10 days, and 35 days at 20°C, 4°C, -20°C, and -80°C, respectively.
CONCLUSION: We recommend to transport antibiotic plasma samples in ice at 4°C and even at -20°C if these samples come from external hospitals. Ideally, plasma samples should be stored at -80°C if possible; if not, the analysis of the samples should be performed as soon as possible in the limit of 10 days after a storage at -20°C.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
|---|---|
| Enthalten in: |
Fundamental & clinical pharmacology - (2023) vom: 20. Nov. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Brenkman, Matthieu [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Beta-lactams |
|---|
| Anmerkungen: |
Date Revised 21.11.2023 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1111/fcp.12969 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM364772042 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM364772042 | ||
| 003 | DE-627 | ||
| 005 | 20231226100130.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/fcp.12969 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1215.xml |
| 035 | |a (DE-627)NLM364772042 | ||
| 035 | |a (NLM)37985813 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Brenkman, Matthieu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a In vitro stability study of 10 beta-lactam antibiotics in human plasma samples |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 21.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a © 2023 Société Française de Pharmacologie et de Thérapeutique. Published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND AND OBJECTIVES: Beta-lactam antibiotics are reported for some of them to be subject to a rapid degradation in infusion solutions and in human blood samples. However, the current data of stability available in blood samples are limited to a few number of beta-lactam antibiotics, and the methodology of the corresponding studies may be discussed. The objective of the present study is to evaluate the stability of 10 beta-lactam antibiotics in human plasma samples | ||
| 520 | |a METHODS: Stability of amoxicillin, cefazolin, cefepime, cefotaxime, cefoxitin, ceftazidime, ceftriaxone, imipenem, meropenem, and piperacillin was evaluated at low and high concentrations at 20°C, 4°C, -20°C, and -80°C for 1, 7, 60, and 90 days, respectively | ||
| 520 | |a RESULTS: Amoxicillin, cefepime, meropenem, and piperacillin were the least stable antibiotics. The maximum durations allowing the stability for all the evaluated beta-lactams at both tested concentrations were estimated at 3 h, 23 h, 10 days, and 35 days at 20°C, 4°C, -20°C, and -80°C, respectively | ||
| 520 | |a CONCLUSION: We recommend to transport antibiotic plasma samples in ice at 4°C and even at -20°C if these samples come from external hospitals. Ideally, plasma samples should be stored at -80°C if possible; if not, the analysis of the samples should be performed as soon as possible in the limit of 10 days after a storage at -20°C | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a beta-lactams | |
| 650 | 4 | |a drug monitoring | |
| 650 | 4 | |a human plasma | |
| 650 | 4 | |a quality assurance | |
| 650 | 4 | |a stability | |
| 700 | 1 | |a Cartau, Tom |e verfasserin |4 aut | |
| 700 | 1 | |a Pape, Elise |e verfasserin |4 aut | |
| 700 | 1 | |a Kolodziej, Allan |e verfasserin |4 aut | |
| 700 | 1 | |a Charmillon, Alexandre |e verfasserin |4 aut | |
| 700 | 1 | |a Novy, Emmanuel |e verfasserin |4 aut | |
| 700 | 1 | |a Jouzeau, Jean-Yves |e verfasserin |4 aut | |
| 700 | 1 | |a Gambier, Nicolas |e verfasserin |4 aut | |
| 700 | 1 | |a Scala-Bertola, Julien |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Fundamental & clinical pharmacology |d 1990 |g (2023) vom: 20. Nov. |w (DE-627)NLM012737992 |x 1472-8206 |7 nnns |
| 773 | 1 | 8 | |g year:2023 |g day:20 |g month:11 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/fcp.12969 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 20 |c 11 | ||