Material extrusion additive manufacturing of poly(lactic acid)/Ti6Al4Vcalcium phosphate core-shell nanocomposite scaffolds for bone tissue applications
Copyright © 2023 Elsevier B.V. All rights reserved..
The use of porous scaffolds with appropriate mechanical and biological features for the host tissue is one of the challenges in repairing critical-size bone defects. With today's three-dimensional (3D) printing technology, scaffolds can be customized and personalized, thereby eliminating the problems associated with conventional methods. In this work, after preparing Ti6Al4V/Calcium phosphate (Ti64CaP) core-shell nanocomposite via a solution-based process, by taking advantage of fused deposition modeling (FDM), porous poly(lactic acid) (PLA)-Ti64@CaP nanocomposite scaffolds were fabricated. Scanning electron microscope (SEM) showed that nanostructured calcium phosphate was distributed uniformly on the surface of Ti64 particles. Also, X-ray diffraction (XRD) indicated that calcium phosphate forms an octacalcium phosphate (OCP) phase. As a result of incorporating 6 wt% Ti64@CaP into the PLA, the compressive modulus and ultimate compressive strength values increased from 1.4 GPa and 29.5 MPa to 2.0 GPa and 53.5 MPa, respectively. Furthermore, the differential scanning calorimetry results revealed an increase in the glass transition temperature of PLA, rising from 57.0 to 62.4 °C, due to the addition of 6 wt% Ti64@CaP. However, it is worth noting that there was a moderate decrease in the crystallization and melting temperatures of the nanocomposite filament, which dropped from 97.0 to 89.5 °C and 167 to 162.9 °C, respectively. The scaffolds were seeded with human adipose tissue-derived mesenchymal stem cells (hADSCs) to investigate their biocompatibility and cell proliferation. Calcium deposition, ALP activity, and bone-related proteins and genes were also used to evaluate the bone differentiation potential of hADSCs. The obtained results showed that introducing Ti64@CaP considerably improved in vitro biocompatibility, facilitating the attachment, differentiation, and proliferation of hADSCs. Considering the findings of this study, the 3D-printed nanocomposite scaffold could be considered a promising candidate for bone tissue engineering applications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:255 |
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| Enthalten in: |
International journal of biological macromolecules - 255(2024) vom: 18. Jan., Seite 128040 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zarei, Masoud [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 10.01.2024 Date Revised 10.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2023.128040 |
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| Förderinstitution / Projekttitel: |
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NLM364728116 |
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| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a The use of porous scaffolds with appropriate mechanical and biological features for the host tissue is one of the challenges in repairing critical-size bone defects. With today's three-dimensional (3D) printing technology, scaffolds can be customized and personalized, thereby eliminating the problems associated with conventional methods. In this work, after preparing Ti6Al4V/Calcium phosphate (Ti64CaP) core-shell nanocomposite via a solution-based process, by taking advantage of fused deposition modeling (FDM), porous poly(lactic acid) (PLA)-Ti64@CaP nanocomposite scaffolds were fabricated. Scanning electron microscope (SEM) showed that nanostructured calcium phosphate was distributed uniformly on the surface of Ti64 particles. Also, X-ray diffraction (XRD) indicated that calcium phosphate forms an octacalcium phosphate (OCP) phase. As a result of incorporating 6 wt% Ti64@CaP into the PLA, the compressive modulus and ultimate compressive strength values increased from 1.4 GPa and 29.5 MPa to 2.0 GPa and 53.5 MPa, respectively. Furthermore, the differential scanning calorimetry results revealed an increase in the glass transition temperature of PLA, rising from 57.0 to 62.4 °C, due to the addition of 6 wt% Ti64@CaP. However, it is worth noting that there was a moderate decrease in the crystallization and melting temperatures of the nanocomposite filament, which dropped from 97.0 to 89.5 °C and 167 to 162.9 °C, respectively. The scaffolds were seeded with human adipose tissue-derived mesenchymal stem cells (hADSCs) to investigate their biocompatibility and cell proliferation. Calcium deposition, ALP activity, and bone-related proteins and genes were also used to evaluate the bone differentiation potential of hADSCs. The obtained results showed that introducing Ti64@CaP considerably improved in vitro biocompatibility, facilitating the attachment, differentiation, and proliferation of hADSCs. Considering the findings of this study, the 3D-printed nanocomposite scaffold could be considered a promising candidate for bone tissue engineering applications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Additive manufacturing | |
| 650 | 4 | |a Bone tissue engineering | |
| 650 | 4 | |a Calcium phosphate | |
| 650 | 4 | |a Fused deposition modeling (FDM) | |
| 650 | 4 | |a Poly(lactic acid)(PLA) | |
| 650 | 4 | |a Polymer-matrix composites (PMCs) | |
| 650 | 7 | |a poly(lactide) |2 NLM | |
| 650 | 7 | |a 459TN2L5F5 |2 NLM | |
| 650 | 7 | |a titanium alloy (TiAl6V4) |2 NLM | |
| 650 | 7 | |a 12743-70-3 |2 NLM | |
| 650 | 7 | |a Polyesters |2 NLM | |
| 650 | 7 | |a Calcium Phosphates |2 NLM | |
| 700 | 1 | |a Hasanzadeh Azar, Mahdi |e verfasserin |4 aut | |
| 700 | 1 | |a Sayedain, Sayed Shahab |e verfasserin |4 aut | |
| 700 | 1 | |a Shabani Dargah, Motahareh |e verfasserin |4 aut | |
| 700 | 1 | |a Alizadeh, Reza |e verfasserin |4 aut | |
| 700 | 1 | |a Arab, Mehdi |e verfasserin |4 aut | |
| 700 | 1 | |a Askarinya, Amirhossein |e verfasserin |4 aut | |
| 700 | 1 | |a Kaviani, Alireza |e verfasserin |4 aut | |
| 700 | 1 | |a Beheshtizadeh, Nima |e verfasserin |4 aut | |
| 700 | 1 | |a Azami, Mahmoud |e verfasserin |4 aut | |
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